Warner Product Videos on JoVE

Dopamine Release at Individual Presynaptic Terminals Visualized with FFNs

Hui Zhang^1,2, Niko G. Gubernator^3,4, Minerva Yue1, Roland G. W. Staal1, Eugene V. Mosharov1, Daniela Pereira1, Vojtech Balsanek³, Paul A. Vadola³, Bipasha Mukherjee⁵, Robert H. Edwards⁵, David Sulzer1^2,6, Dalibor Sames³

¹Departments of Neurology, Columbia University, ²Departments of Psychiatry and Pharmacology, Columbia University, ³Department of Chemistry, Columbia University, ⁴eMolecules, Inc., ⁵Departments of Neurology and Physiology, University of California School of Medicine, San Francisco, ⁶Division of Molecular Therapeutics, New York Psychiatric Institute

A new means to measure neurotransmission optically using fluorescent dopamine analogs.

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• RC-26GLP
• PM platforms
• Suction tubes
• Slice anchors (harps)

Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes

Eiji Shigetomi, Baljit S. Khakh

Departments of Physiology and Neurobiology, David Geffen School of Medicine, University of California, Los Angeles

We describe how to measure near membrane and global intracellular calcium dynamics in cultured astrocytes using total internal reflection and epifluorescence microscopy.

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• RC-21BRW
• PM Platforms
• Stage adapters

Calcium Imaging of Cortical Neurons using Fura-2 AM

Odmara L Barreto-Chang, Ricardo E Dolmetsch

Department of Neurobiology, Stanford University

Calcium signals play a key role in many cellular processes including gene expression, survival and differentiation. Here we demonstrate how to perform calcium imaging using Fura-2 AM. Calcium imaging is a valuable tool to study the regulation of intracellular calcium in real time and its regulation of signaling cascades.

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• Series 20 Chambers
• PM Platforms
• Stage adapters

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

Javeed Shaikh Mohammed¹, Hugo Caicedo¹, Christopher P. Fall², David T. Eddington^1
1Dept. of Bioengineering, University of Illinois, Chicago, ²Department of Anatomy and Cell Biology, University of Illinois, Chicago

We demonstrate fabrication of a simple microfluidic device that can be integrated with standard electrophysiology setups to expose microscale surfaces of a brain slice in a well controlled manner to different neurotransmitters.

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• RC-26G
• PM Platforms
• Stage adapters