Designed to provide complete environmental control over 35 mm culture dishes while maintaining the ability to make both short- and long-term imaging studies.
- Designed for glass-bottomed 35 mm cell culture dishes
- Permits imaging, temperature, and gas atmosphere control
- Adapts to Willco Wells, and Corning and Falcon dishes
- Unique dish clamps permit either open or closed use
- Works with RC-37 family of Cell Culture Dish Perfusion Chamber Inserts
The introduction of glass-bottomed 35 mm culture dishes extended their use into areas such as confocal microscopy, fluorescence microscopy, and high resolution image analysis. The DH-35i Culture Dish Incubator is a system designed for imaging live cells in an open culture dish for easy access or in a closed dish for gas atmosphere control. DH-35i Incubators work well with Willco Wells, Corning and Falcon glass bottom 35 mm culture dishes. Adapter rings are supplied to ensure a close fit for the supported dish. Dishes can be placed in the holder with or without dish covers.
The culture dish holder is machined in three pieces. The midsection and top are easily removed for dish replacement and enables the positioning of electrodes at low angles. Resistive heating of the DH-35i is provided via the optional Warner TC-324B and TC-344B Temperature Controllers and a CC-28 cable.
Clamps are provided to ensure good contact between the dish and the heated surface and to prevent unwanted movement. The removable midsection is magnetically secured and has several ports to allow multi-channel perfusion, atmospheric maintenance, and aspiration of the dish. The base of the holder has a 19 mm aperture and the removable cover features a 36 mm glass aperture.
When combined with Warner's RC-37 Cell Culture Perfusion Chamber Insert, a complete micro-environmental system is achieved. This system is compatible with our SH-27B and SF-28 In-line Solution Heaters. Included with the system are an MP-2 perfusion manifold and a set of 4 culture dish adapter rings. Stage adapters are available for all major microscopes and must be purchased separately. Custom or modified stage adapters are also available. |
RC-37 Insert shown but purchased separately
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Designed to provide complete environmental control over 35 mm culture dishes while maintaining the ability to make both short- and long-term imaging studies.
- Designed for glass-bottomed 35 mm cell culture dishes
- Permits imaging, temperature, and gas atmosphere control
- Adapts to Willco Wells, and Corning and Falcon dishes
- Unique dish clamps permit either open or closed use
- Works with RC-37 family of Cell Culture Dish Perfusion Chamber Inserts
AR-4 Ring Dimensions
| Color | ID | OD |
| Gold | 1.534 | 1.599 |
| Green | 1.415 |
| Blue | 1.331 |
| Red | 1.474 |
| Black | 1.396 |
| Silver | 1.394 |
| Order No. |
Model No. |
Description |
Ordering |
| 64-0107 |
TA-29 |
Cable with bead thermistor |
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| 64-0106 |
CC-28 |
Cable assembly for heater controllers |
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| 64-0351 |
AR-6 |
Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 |
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| 64-1633 |
TCM-1 |
Thermal Cooling Module |
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| 64-0347 |
RC-37W |
Chamber Insert for Willco 35 mm cell culture dishes |
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| 64-0348 |
RC-37F |
Chamber Insert for Corning, MatTek, Nunc, WPI, and Falcon 35 mm cell culture dishes |
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| 64-0100 |
TC-324B |
TC-324B Heater Controller, single channel |
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| 64-0101 |
TC-344B |
TC-344B Heater Controller, dual channel |
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| 64-0102 |
SH-27B |
In-line solution heater |
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| 64-0103 |
SF-28 |
Slow-Flow in-line solution heater |
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| 64-1543 |
HCS-1 |
Heated top coverslip for DH-35i and DH-40i chamber |
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| 64-1544 |
HCS-RPL |
Replacement ITO coverslip for HCS-1 heated chamber top |
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| 64-1507 |
RCP-2 |
Replacement Clamps for DH-35i, DH-40i and QE-1 |
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Reference Articles
- Extracellular ATP dissociates nonmuscle myosin from P2X7 complex: this dissociation regulates P2X7 pore formation
Ben J Gu, Catherine Rathsam, Leanne Stokes, Andrew B McGeachie, and James S. Wiley
Am J Physiol Cell Physiol (June 3, 2009)
- Talin phosphorylation by Cdk5 regulates Smurf1-mediated talin head ubiquitylation and cell migration
Huang C, Rajfur Z, Yousefi N, Chen Z, Jacobson K, Ginsberg MH
Nat Cell Biol. 2009 May;11(5):624-30. Epub 2009 Apr 12
- Single-Cell Transfection by Electroporation Using an Electrolyte/Plasmid-Filled Capillary
Wang M, Orwar O, Weber SG
Anal Chem. 2009 May 15;81(10):4060-7
- Retinoic Acid-induced Gene-1 (RIG-I) Associates with the Actin Cytoskeleton via Caspase Activation and Recruitment Domain-dependent Interactions
Mukherjee A, Morosky SA, Shen L, Weber CR, Turner JR, Kim KS, Wang T, Coyne CB
J Biol Chem. 2009 Mar 6;284(10):6486-94. Epub 2009 Jan 3
- Monitoring dynamic protein expression in living E. coli. Bacterial cells by laser tweezers Raman spectroscopy
Chan JW, Winhold H, Corzett MH, Ulloa JM, Cosman M, Balhorn R, Huser T
Cytometry A. 2007 Jul;71(7):468-74
- Effect of Cell Size and Shape on Single-Cell Electroporation
Agarwal A, Zudans I, Weber EA, Olofsson J, Orwar O, Weber SG
Anal Chem. 2007 May 15;79(10):3589-96. Epub 2007 Apr 20
- Experimentally Determining the IR Drop in Solution at Carbon Fiber Microelectrodes with Current Interruption and Application to Single-Cell Electroporation
Lambie BA, Brennan C, Olofsson J, Orwar O, Weber SG
Anal Chem. 2007 May 15;79(10):3771-8. Epub 2007 Apr 6
- Simultaneous maximization of cell permeabilization and viability in single cell electroporation using an electrolyte-filled capillary
Agarwal A, Zudans I, Orwar O, Weber SG
Anal Chem. 2007 Jan 1;79(1):161-7
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Order No. (Model No.) |
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Product Description |
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USA Price |
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Order |
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64-0349 (DH-35i) |
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Culture dish incubation system |
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Log In |
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Log In |
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Accessories and Replacement Parts |
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64-0351 (AR-6) |
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Adapter ring kit for DH-35i or QE-1, fits Willco, Corning, and Falcon dishes, set of 6 |
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Log In |
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Log In |
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64-0347 (RC-37W) |
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Chamber Insert for Willco 35 mm cell culture dishes |
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Log In |
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Log In |
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64-0348 (RC-37F) |
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Chamber Insert for Corning, MatTek, Nunc, WPI, and Falcon 35 mm cell culture dishes |
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Log In |
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Log In |
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64-1633 (TCM-1) |
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Thermal cooling module, heat exchanger |
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Log In |
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Log In |
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64-0100 (TC-324B) |
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TC-324B Heater Controller, single channel |
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Log In |
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Log In |
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64-0101 (TC-344B) |
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TC-344B Heater Controller, dual channel |
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Log In |
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Log In |
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64-0106 (CC-28) |
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Cable assembly for heater controllers |
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Log In |
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Log In |
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64-0107 (TA-29) |
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Cable with bead thermistor |
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Log In |
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Log In |
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64-0102 (SH-27B) |
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In-line Solution Heater |
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Log In |
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Log In |
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64-0103 (SF-28) |
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Slow Flow In-Line Solution Heater |
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Log In |
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Log In |
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64-1543 (HCS-1) |
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Heated top coverslip for DH-35i and DH-40i chamber |
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Log In |
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Log In |
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64-1544 (HCS-RPL) |
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Replacement ITO coverslip for HCS-1 heated chamber top |
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Log In |
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Log In |
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64-1507 (RPC-2) |
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Replacement clamps for DH-35i, DH-40I, QE-1 |
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Log In |
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Log In |
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