Specialized Tools for Electrophysiology and Cell Biology Research

Classic Bilayer Cups & Chambers

Product Summary

This 2-part system consists of a black Delrin chamber and a cuvette of either polystyrene, polysulfone or Delrin (cups purchased separately). Cups and chambers are designed such that addition of equal volumes to the cup and chamber results in a balanced solution height, minimizing any pressure gradients across the bilayer membrane.

  • Cuvettes in Delrin, Polystyrene, or Polysulfone
    (colored white, translucent or honey, respectively)
  • Precision Machined Apertures 150, 200 or 250 µm
  • Viewing Window
  • cis and trans stirbar wells
  • Stirbars Included with each cup and chamber
  • Shipments from Stock

The Basics

Cups and chambers from Warner Instruments are designed such that addition of equal volumes to the cis and trans sides results in a balanced solution height, thus minimizing mechanical gradients across the bilayer membrane.

Bilayer Cuvettes

Polystyrene has been a favored material for cuvettes for several years. The physical properties of this material make for a high quality membrane support. However, its poor resistance to organic solvents can lead to degradation of the aperture. Cuvettes have also been made from Delrin (acetyl resin) and many users report good membrane formation as well as easier maintenance and longer life. It should be noted, however, that Delrin cuvettes do not work equally well in all applications and with all users.

Warner Instruments now offers bilayer cups made from a new material, polysulfone. This material has many of the mechanical properties of polystyrene coupled with enhanced solvent resistance. The choice of material will depend on your application.

All cuvettes have a 0.5 mm well milled into their base to confine the motion of stirbars and to reduce mechanical noise artifacts. A 2 x 5 mm or 2 x 7 mm PTFE-coated stirbar is supplied with each cuvette.

Standard available aperture diameters are 150, 200 or 250 µm. Contact our offices for custom aperture sizes from 50 µm to 1 mm. The wall thickness at the aperture is 250 µm.

Bilayer Chambers

Chambers are made from black Delrin and also have a 0.5 mm recessed well milled into the floor of the non-cup side to confine the motion of stirbars and to reduce mechanical noise artifacts. A 2 x 5 mm or 2 x 7 mm PTFE-coated stirbar is supplied with each chamber.

Polyethylene centrifuge tubes (supplied) serve as intermediate wells for electrical connections between the headstage electrodes and the cup or chamber. Chambers include a nylon screw and rubber plug to secure the cups during use. The classic model has a polycarbonate window for viewing the aperture during membrane formation.

Classic Models BCH-M13 and BCH-M22

Two classic models are offered: The BCH-M13 is a small volume chamber (1.2 ml) with a 13 mm (1/2") diameter cuvette. The BCH-M22 is a larger volume chamber with (5.0 ml) chamber with a 22 mm (7/8") diameter cuvette.

References

  1. The multimeric structure of polycystin-2 (TRPP2): structural–functional correlates of homo- and hetero-multimers with TRPC1 
    Zhang P, Luo Y, Chasan B, González-Perrett S, Montalbetti N, Timpanaro GA, Cantero Mdel R, Ramos AJ, Goldmann WH, Zhou J, Cantiello HF
    Hum Mol Genet. 2009 Apr 1;18(7):1238-51. Epub 2009 Feb 3
  2. Reactive Oxygen Species Inhibit Polycystin-2 (TRPP2) Cation Channel Activity In Term Human Syncytiotrophoblast 
    Montalbetti N, Cantero MR, Dalghi MG, Cantiello HF 
    Placenta. 2008 Jun;29(6):510-8. Epub 2008 Apr 15
  3. Isolated Ocular Disease Is Associated with Decreased Mucolipin-1 Channel Conductance 
    Ehud Goldin, Rafael C. Caruso, William Benko, Christine R. Kaneski, Stephanie Stahl, and Raphael Schiffmann 
    Invest Ophthalmol Vis Sci. 2008 Jul;49(7):3134-42. Epub 2008 Mar 7
  4. Polycystin-2 cation channel function in the human syncytiotrophoblast is regulated by microtubular structures 
    Montalbetti N, Li Q, Wu Y, Chen XZ, Cantiello HF
    J Physiol. 2007 Mar 15;579(Pt 3):717-28. Epub 2007 Jan 4
  5. Cytoskeletal regulation of calcium-permeable cation channels in the human syncytiotrophoblast: role of gelsolin 
    Nicolás Montalbetti, Qiang Li, Gustavo A Timpanaro, Silvia González-Perrett, Xiao-Qing Dai, Xing-Zhen Chen and Horacio F Cantiello, 
    J. Physiol., March 15, 2007; 579(3): 717 - 728
  6. Polycystin-2 Cation Channel Function Is under the Control of Microtubular Structures in Primary Cilia of Renal Epithelial Cells 
    Qiang Li, Nicola´s Montalbetti, Yuliang Wu, Arnolt Ramos, Malay K. Raychowdhury, Xing-Zhen Chen, and Horacio F. Cantiello, 
    THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 49, pp. 37566–37575, December 8, 2006
  7. Ca2-dependent Control of the Permeability Properties of the Mitochondrial Outer Membrane and Voltage-dependent Anion-selective Channel (VDAC) 
    Báthori G, Csordás G, Garcia-Perez C, Davies E, Hajnóczky G
    THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 25, pp. 17347–17358, June 23, 2006
  8. Ca2+-Induced Ca2+ Release through Localized Ca2+ Uncaging in Smooth Muscle 
    Ji G, Feldman M, Doran R, Zipfel W, Kotlikoff MI
    J Gen Physiol. 2006 Mar;127(3):225-35
  9. Ion Channel Conductance Measurements on a Silicon-Based Platform
    S. J. Wilk, S. Aboud, L. Petrossian, M. Goryll, J. M. Tang, R. S. Eisenberg, M. Saraniti, S. M. Goodnick, T. J. Thornton 
    Journal of Physics: Conference Series 38 (2006) 21–24
  10. Neuronal calcium sensor-1 enhancement of InsP3 receptor activity is inhibited by therapeutic levels of lithium 
    Christina Schlecker, Wolfgang Boehmerle, Andreas Jeromin, Brenda DeGray, Anurag Varshney, Yogendra Sharma, Klara Szigeti-Buck and Barbara E. Ehrlich, 
    J. Clin. Invest. 116:1668-1674 (2006)
  11. Hydroxylated xestospongins block inositol-1,4,5-trisphosphate-induced Ca2+ release and sensitize Ca2+-induced Ca2+ release mediated by ryanodine receptors 
    Ta TA, Feng W, Molinski TF, Pessah IN 
    Mol Pharmacol. 2006 Feb;69(2):532-8. Epub 2005 Oct 25
  12. Calcium and chloride channel activation by angiotensin II-AT1 receptors in preglomerular vascular smooth muscle cells 
    Fuller AJ, Hauschild BC, Gonzalez-Villalobos R, Awayda MS, Imig JD, Inscho EW, Navar LG 
    Am J Physiol Renal Physiol. 2005 Oct;289(4):F760-7. Epub 2005 Jun 7
  13. A Large, Voltage-Dependent Channel, Isolated from Mitochondria by Water-Free Chloroform Extraction 
    Pavlov E, Zakharian E, Bladen C, Diao CT, Grimbly C, Reusch RN, French RJ 
    Biophys J. 2005 Apr;88(4):2614-25. Epub 2005 Feb 4
  14. Mechanisms of Quantum Dot Interactions with Biological Cells
    Jay L. Nadeaua, Samuel J. Clarkea, Rafael A. Khatchadouriana, Daniel M. Bahchelia, Diana Suffernb, Stephen E. Bradforthb, 
    Progress in biomedical optics and imaging, 2005
  15. Ion Channels on Silicon
    S. J. Wilk, L. Petrossian, M. Goryll, T. J. Thornton, and S. M. Goodnick J. M. Tang and R. S. Eisenberg M. Saraniti D. Wong, J. J. Schmidt, and C. D. Montemagno 
    e-J. Surf. Sci. Nanotech. Vol. 3 (2005) 184-189

Order No. Model No. Product Description USA Price Order
64-0451 BCH-M13 Bilayer chamber, 1.0 ml working volume, with stirbar, without cuvette Login Login
64-0404 CP13A-150 Polystyrene cuvette, 1.0 ml working volume, with 150 µm aperture Login Login
64-0403 CP13A-200 Polystyrene cuvette, 1.0 ml working volume, with 200 µm aperture Login Login
64-0402 CP13A-250 Polystyrene cuvette, 1.0 ml working volume, with 250 µm aperture Login Login
64-0410 CD13A-150 Delrin cuvette, 1.0 ml working volume, with 150 µm aperture Login Login
64-0409 CD13A-200 Delrin cuvette, 1.0 ml working volume, with 200 µm aperture Login Login
64-0408 CD13A-250 Delrin cuvette, 1.0 ml working volume, with 250 µm aperture Login Login
64-0416 CF13A-150 Polysulfone cuvette, 1.0 ml working volume, with 150 µm aperture Login Login
64-0415 CF13A-200 Polysulfone cuvette, 1.0 ml working volume, with 200 µm aperture Login Login
64-0414 CF13A-250 Polysulfone cuvette, 1.0 ml working volume, with 250 µm aperture Login Login
64-0453 BCH-M22 Bilayer chamber, 3.0 ml working volume, with stirbar, without cuvette Login Login
64-0407 CP22A-150 Polystyrene cuvette, 3.0 ml working volume, with 150 µm aperture Login Login
64-0406 CP22A-200 Polystyrene cuvette, 3.0 ml working volume, with 200 µm aperture Login Login
64-0413 CD22A-150 Delrin cuvette, 3.0 ml working volume, with 150 µm aperture Login Login
64-0412 CD22A-200 Delrin cuvette, 3.0 ml working volume, with 200 µm aperture Login Login
64-0411 CD22A-250 Delrin cuvette, 3.0 ml working volume, with 250 µm aperture Login Login
64-0419 CF22A-150 Polysulfone cuvette, 3.0 ml working volume, with 150 µm aperture Login Login
64-0417 CF22A-250 Polysulfone cuvette, 3.0 ml working volume, with 250 µm aperture Login Login
64-0420 MAG-13 Stir Bars, 2 x 5mm for 1 ml cuvettes, 5 pack Login Login
64-0421 MAG-22 Stir Bars, 2 x 7mm for 3 ml cuvettes, 5 pack Login Login
64-1729 BCH-13TH Thumb screw and rubber plug for BCH-M13 and BCH-M22 chambers Login Login
Classic 22 mm Cups
64-0405 CP22A-250 Polystyrene cuvette, 3.0 ml working volume, with 250 µm aperture Login Login
64-0418 CF22A-200 Polysulfone cuvette, 3.0 ml working volume, with 200 µm aperture Login Login

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