Specialized Tools for Electrophysiology and Cell Biology Research

Classic Ussing Systems

The Classic Ussing design presented here varies little from the original equipment created by Dr. Hans Ussing. The system's low initial cost, coupled with the modest cost of add-on components, makes it an ideal option for classroom instruction and in-the-field research.

  • Classic system as designed by DR. H. H. Ussing
  • Supports membranes from 2.5 to 15.5 mm wide
  • Supports Snapwell, Transwell, and Multiwell culture cups
  • Insert version available
  • Compatible with UssingChart hardware and software

The investigation of ionic transport across epithelial cells was greatly facilitated by the development of a chamber system designed by Dr. H. H. Ussing more than 40 years ago. Today this equipment bears his name.

The U9500 ‘Classic’ design presented here varies little from Dr. Ussing’s original equipment which remains in wide use for the study of electrophysiological properties of epithelial tissues. A newer design, the U9520 ‘Classic with Inserts’ allows for insertion of cell culture or tissue supports and greatly increases the functionality of the classic Ussing chamber. The low initial cost of the classic design, coupled with the modest cost of add-ons, makes this system an ideal option for classroom instruction and in-the-field research.

The Classic Ussing Systems

The U9500 and U9520 are complete Ussing systems consisting of an Ussing chamber, chamber support stand, circulation reservoir, utility clamp, air/gas fitting kit, and electrode set. The U9500 chamber uses a non-insert design, while the the U9520 uses an insert design. Both systems are otherwise identical with the difference embodied in the chamber.

These systems are fully compatible with the UssingChart data acquisition package which consists of an EC-825A amplifier, LIH 8+8 digitizer, UssingChart software, and connecting cables.

U9500 Classic Chambers

The U9500 chambers are machined from solid acrylic into two halves, each with vertical and horizontal ports for connection to the circulation system and for making electrical connections.

One chamber half-face is imbedded with sharp stainless-steel pins that mate with corresponding holes in the other chamber half-face. This allows for puncturing and positioning the epithelium membrane within the chamber. Chambers are available in a variety of aperture sizes and pin configurations.

In the U9500 design, a separate chamber must be purchased for each desired aperture size. This is a good choice if you intend to study a single tissue type as assembly is both direct and simple.

U9520 'Classic with Inserts' Chambers

The U9520 chambers are functionally similar to the U9500 series except that it makes use of low-cost adapters (ie, inserts) to position tissues or cultured cells within the chamber body. Inserts are interchangeable and permit the researcher to investigate a variety of tissues without having to purchase a separate chamber for each different tissue. The U9520 chamber is mounted in the standard support stand and procedures for experimentation are the same as with the classic system.

Chamber and Insert Aperture Configurations

U9500 chambers are available with pinned openings in diameters from 3.8 to 13.5 mm and in slotted widths from 1.6 to 3.5 mm to accommodate a variety of tissue dimensions. The choice of pin configuration depends on the epithelia under study.

The specified diameter designates the main opening to which the buffer makes contact with the membrane. Select an aperture opening at least 2 mm smaller (1 mm all the way around) to permit the pins to impale the tissue without tearing.

U9520 chamber inserts are available with openings in diameters from 3.8 to 13.5 mm. Inserts are also available for culture cups from Falcon, Millicell, Snapwell, and Transwell.

Both U9500 and U9520 chambers are available with and without drains.

Circulation Reservoir

Available 10 ml capacity, the circulation reservoir is a two sided, hand-blown apparatus made from borosilicate glass. Each side has two outlets at the base for connection to the perfusion fittings on the associated Ussing chamber half-cell. Each side also has a separate air/gas inlet to drive the circulation system. Gas, commonly a 95% O2/5% CO2 mixture, is forced under low pressure into these inlets and allowed to come into contact with the buffer solution. The rising bubbles both drive circulation and oxygenate the buffer. This method is commonly referred to as a 'gas-lift' perfusion system.

Each horizontal reservoir arm has either an inlet or outlet port for access to the water jacket. Water at the desired temperature is pumped through the jacket and allows thermoregulation of the perfusion solutions. Glass condensers are provided to minimize evaporative solution losses from the top.

Electrode Sets and Agar Bridge Fittings

Each System is supplied with a U9975A Electrode Set comprised of 4 electrodes, 2 Ag-AgCl pellet electrodes (for voltage) and 2 Ag wire electrodes (for current). Electrodes include 1 meter (3 feet) of wire and a connector to plug into the amplifier headstage. The EC-825A or EC-800 amplifier can be added to the Ussing chamber to perform electrophysiology studies.

Also supplied are Luer taper fittings that screw onto the electrodes to provide an agar salt bridge connection into the chamber. Fittings can be filled with agar either before or after assembly onto the electrodes.

Data Acquisition

The U9500 and U9520 Classic Ussing systems are compatible with electrophysiological epithelial transport measurements and use UssingChart data acquisition. This package includes an amplifier, a digitizer, software, and cables. You can view details on the UssingChart Data Acquisition webpage.

Classic Ussing System Expandability

The Classic Ussing system can easily be scaled to two channels by adding another Classic or Classic with Insert chamber system.
Refer to the chart below for the needed components:

Expansion Needed items Order No. Quantity to order
Increase to 2 channels Classic Ussing chamber base assembly
does not include chamber and/or insert (for Classic with Insert) - order these separately
64-0500R10 1

For all chambers not using culture cups:

Increase the tissue dimensions by a minimum of 3.0 mm in each direction
to allow the o-ring or pins to capture the tissue without tearing.

For example, a chamber with a 5 mm round aperture will require a tissue with a minimum diameter of 8 mm,
and a chamber with a 2 x 10 mm oblong aperture will require a tissue with minimum dimension of 5 x 13 mm.

Suggested application(s) by aperture

Aperture Applications Aperture area
3.8 mm round with pins biopsy
general use
0.11 cm2
6 mm round with pins general use 0.28 cm2
9 mm round with pins general use 0.64 cm2
12 mm round with pins general use 1.13 cm2
13.5 mm round with pins general use 1.43 cm2
1.5 x 7 mm oblong with pins mouse trachea
rat trachea
0.11 cm2
2.5 x 7 mm oblong with pins mouse intestine
rabbit trachea
0.18 cm2
3.5 x 7 mm oblong with pins mouse intestine
rat intestine
rabbit intestine
0.25 cm2
9 mm round with o-rings general use 0.64 cm2
12 mm round with o-rings general use
skin
cornea
1.13 cm2
13.5 mm round with o-rings general use
skin
cornea
1.43 cm2
Snapwell, 12.7 mm cell culture 1.12 cm2
Millicell, 12 mm cell culture 1.13 cm2
Transwell, 6.5 mm cell culture 0.33 cm2
Transwell 12 mm cell culture 1.13 cm2
Transwell, 24 mm cell culture 4.52 cm2

Pricing Top Description

How to order:

  • Select Base Assembly 
    Systems include support stand with rods circulation reservoir; utility clamp; U9303 Air/Gas Fitting Kit; U9975A Electrode Set and U9965EP Electrode Bridge Fitting Kit
  • Select chamber 
    Choices are 'Classic' or 'Classic with Insert'. Both available with or without drains
  • Select insert 
    If using 'Classic with Insert'
  • Select Data Acquisition package for Ussing (UssingChart)
    If making electrophysiological measurements
  • Select thermocirculator and Tubing Kit
    If temperature regulation is needed

Order No. Model No. Product Description USA Price Order
'Classic with Insert' Chamber System (Chambers)
64-0532 U9521 Cell culture Ussing chamber Login Login
64-0533 U9521DR Cell culture Ussing chamber, with drains Login Login
'Classic without Insert' Chamber System ('Classic' Without drains)
64-0511 U9504SP-3 Slotted chamber 3.5 x 7.0 mm aperture, 0.25 cm2 area Login Login
64-0508 U9513 Ussing Chamber, 13.5 mm aperture, 1.43 cm2 area Login Login
64-0505 U9506 Ussing Chamber, 6.0 mm aperture, 0.28 cm2 area Login Login
64-0510 U9504SP-2 Slotted chamber, 2.5 x 7.0 mm aperture, 0.18 cm2 area Login Login
64-0507 U9512 Ussing Chamber, 12.0 mm aperture, 1.13 cm2 area Login Login
64-0509 U9504SP-1 Slotted chamber, 1.5 x 7.0 mm aperture, 0.11 cm2 area Login Login
64-0506 U9509 Ussing Chamber, 9.0 mm aperture, 0.64 cm2 area Login Login
'Classic' With drains
64-0517 U9504SP-1DR Slotted chamber, 1.5 x 7.0 mm aperture, 0.11 cm2 area Login Login
64-0514 U9509DR Ussing Chamber, 9.0 mm diameter aperture, 0.64 cm2 area Login Login
64-0519 U9504SP-3DR Slotted chamber, 3.5 x 7.0 mm aperture, 0.25 cm2 area Login Login
64-0516 U9513DR Ussing Chamber, 13.5 mm diameter aperture, 1.43 cm2 area Login Login
64-0513 U9506DR Ussing Chamber, 6.0 mm diameter aperture, 0.28 cm2 area Login Login
64-0518 U9504SP-2DR Slotted chamber, 2.5 x 7.0 mm aperture, 0.18 cm2 area Login Login
64-0515 U9512DR Ussing Chamber, 12.0 mm diameter aperture, 1.13 cm2 area Login Login
64-0512 U9504DR Ussing Chamber, 3.8 mm diameter aperture, 0.11 cm2 area Login Login
Accessories and Replacement Parts
64-0565 U9406 Tubing kit (hose and Luer fittings) Login Login
64-0562 U9403 Support stand Login Login
64-0560-10ml Condenser set for 10 ml circulation reservoir, set of 2 Login Login
64-0553 U9565EP Electrode bridge fitting kit, 12 pack with 6 adapters Login Login
64-0554 U9975A Electrode set, includes 2 Ag/AgCl pellets and 2 Ag wires Login Login
64-0564 U9404 Utility Clamp Login Login
64-0561 U9303 Air/gas connection kit Login Login
64-0560-4ml Condenser set for 4 ml circulation reservoir, set of 2 Login Login
64-0554W Current electrodes, Ag wires, 2 mm pin, 4 pack Login Login
64-0165 SL-6 Luer stopcock, 6 pack Login Login
64-0563 U9403R Support stand rod Login Login
64-0560-20ml Condenser set for 20 ml circulation reservoir, set of 2 Login Login
64-0555 U9985 Bridge fitting adapters, 6 pack Login Login
64-0554P Voltage electrodes, Ag/AgCl pellet, 2 mm pin, 4 pack Login Login
Accessories and Replacement Parts - Thermocirulator
72-4924 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz Login Login
64-2244 WB-KIT TC-120 Thermocirculator Tubing Kit Login Login
72-4921 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz Login Login
Base Assembly
64-0500R10 U950010R Classic Ussing chamber base assembly, includes 10 ml/side circulation reservoir Login Login
Data Acquisition
89-5035 LIH 8+8 LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE Login Login
Data Acquisition - 2 Channel UssingChart package (order 1 of each)
64-3009 EC-825A cable, dual - fits 2 amplifiers (4 acquisition channels) Login Login
Data Acquisition-2 Channel Data Acquisition package (UssingChart) - order 1 of each
89-5285 UssingChart software Login Login
64-3009 EC-825A cable, dual - fits 2 amplifiers (4 acquisition channels) Login Login
64-1605 EC-825A Dual channel epithelial voltage clamp, ±50V compliance, 110-250 VAC Login Login
Inserts for culture cups
64-0552 U9524T-24 Transwell culture cup adapter, 24.0 mm, 4.52 cm2 area Login Login
64-0547 U9524M-12 Millicell culture cup adapter, 12.0 mm, 1.13 cm2 area Login Login
64-0551 U9524T-12 Transwell culture cup adapter, 12.0 mm, 1.13 cm2 area Login Login
64-0549 U9524S Snapwell culture cup adapter, 12.7 mm, 1.12 cm2 area Login Login
64-0550 U9524T-06 Transwell culture cup adapter, 6.50 mm, 0.33 cm2 area Login Login
Inserts with o-rings
64-0538 U9524A-13 Tissue adapter insert with o-ring, 13.5 mm diameter aperture, 1.43 cm2 area Login Login
64-0537 U9524A-12 Tissue adapter insert with o-ring, 12.0 mm diameter aperture, 1.13 cm2 area Login Login
64-0536 U9524A-09 Tissue adapter insert with o-ring, 9.0 mm diameter aperture, 0.64 cm2 area Login Login
Inserts with pins
64-0543 U9524B-13 Tissue adapter insert with pins, 13.5 mm diameter aperture, 1.43 cm2 area Login Login
64-0540 U9524B-06 Tissue adapter insert with pins, 6.0 mm diameter aperture, 0.28 cm2 area Login Login
64-0542 U9524B-12 Tissue adapter insert with pins, 12.0 mm diameter aperture, 1.13 cm2 area Login Login
64-0539 U9524B-04 Tissue adapter insert with pins, 3.8 mm diameter aperture, 0.11 cm2 area Login Login
64-0541 U9524B-09 Tissue adapter insert with pins, 9.0 mm diameter aperture, 0.64 cm2 area Login Login

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