Specialized Tools for Electrophysiology and Cell Biology Research

(RC-30HV, RC-30WA) Confocal Imaging Chambers

The RC-30 is a low profile closed bath chamber specifically designed for confocal imaging applications. Compatible with either upright or inverted microscopes.

  • Option of confocal imaging with continuous perfusion
  • Accommodates both upright and inverted microscopes
  • 17.7 mm diameter viewing aperture (RC-30, RC-30HV)
  • 25.0 mm diameter viewing aperture (RC-30WA)
  • User defined cross-sectional viewing area
  • User defined bath volume with fast fluid exchange
  • Three available gasket thicknesses
  • Resistive heating on chamber base
The RC-30HV and RC-30WA are low profile, closed bath chambers incorporating special features optimized for confocal imaging. Both chambers allow user defined bath geometry and have a large viewing area.

In both designs, #1 thickness glass coverslips form the chamber top and bottom, creating the closed bath. The closed design allows the chamber to be inverted in the mounting platform enabling its use with either inverted or upright microscopes.

The RC-30HV uses a 22x30 mm coverslip for the chamber top and a 22x40 mm coverslip for the bottom. The final viewing aperture is 17.7 mm.

The RC-30WA is a wide aperture version of the RC-30HV. It uses a 40 mm round coverslip for the bottom surface and a 30 mm round coverslip for the top. The final viewing aperture for this chamber is 25 mm.

Variable Bath Volume/Fast Perfusate Exchange

The side walls of the RC-30HV and RC-30 WA bath are formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 125-1000 µm and is defined by the thickness and number of gaskets used. Pre-cut, as well as blank gaskets are supplied with the chamber allowing the development of customized bath geometries as needed.

The supplied pre-cut gaskets include a wide cut gasket to allow for the full use of the aperture window (17.7 mm diameter) and a narrow, slotted version for fast solution exchange. The bath volumes for pre-cut, 250 µm thick gaskets are 133 µl for the wide cut and 26 µl for the slotted cut.

Large Viewing Area

The viewing aperture in the chamber housing is 17.7 mm in diameter. The chamber bottom is beveled to permit maximum access to the bottom coverslip.

Reversible

The RC-30 Series chambers can be mounted right-side up or up-side down in the stage adapter to accommodate use on either upright and inverted microscopes.

Heating

The RC-30HV and RC-30WA include a pair of resistive heating elements mounted to the chamber base. These elements are compatible with Warner's TC-344C and TC-324C temperature controllers which are ordered separately along with a CC-28 cable.

All versions of the RC-30 chambers require special Series 30 microscope stage adapters which also must be ordered separately.

Confocal imaging chambers are supplied with six packages of pre-cut gaskets: two large area bath gaskets (GS-30L/10, GS-30L/15), two slotted bath gaskets (GS-30S/10), and two blank gaskets (GS-30B/10, GS-30B/15) for user customized chamber areas. Also included is a special tool used for mounting the top plate, polyethylene tubing (PE-90/10, 10 feet.), and 22x30 mm and 22x40 mm #1 glass coverslips (CS-22/30 and CS-22/40, pkg. of 50). The RC-30WA includes one box each of 30 mm round coverslips, (CS-30R, and 40 mm round coverslips, CS-40R). Warner offers 125, 250, and 375 µm thick gaskets which are listed in the order information section.

 

See Related Files to download a data sample
collected using the RC-30 chamber (47 MB)

 

Physical Dimensions (H X D) 9.5 x 82 mm
Weight <90 g, assembled with optional heaters
Base and Pressure Plate Aluminum, black anodized
Top Coverslip Plate Polycarbonate
Gaskets Silicone rubber
Input/Output Tubing PE-90
Coverslips (#1) Top: 22 x 30 mm; Bottom: 22 x 40 mm
Microscope Stage Adapters* All major models:
Leica, Nikon, Zeiss, Olympus, Prior, Ludl, Burleigh

 

*Note: Uses Series 30 stage adapters. 
For exterior dimensions use exterior dimension information for corresponding Series 20 adapters. 

 

Order No. Model No. Product Description USA Price Order
64-0754 PE-90/10 Polyethylene tubing, PE-90, 10 ft Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login

References

References

  1. Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen–antibody interaction
    Cao C, Sim SJ
    Lab Chip. 2009 Jul 7;9(13):1836-9
  2. Elevated hydrostatic pressure activates sodium-hydrogen exchanger-1 in rat optic nerve head astrocytes
    Mandal A, Shahidullah M, Delamere NA, Terán MA
    Am J Physiol Cell Physiol. 2009 Jul;297(1):C111-20
  3. Sodium/bicarbonate cotransporter NBCn1/slc4a7 increases cytotoxicity in magnesium depletion in primary cultures of hippocampal neurons
    Cooper DS, Yang HS, He P, Kim E, Rajbhandari I, Yun CC, Choi I
    Eur J Neurosci. 2009 Feb;29(3):437-46
  4. Silencing of ZnT1 reduces Zn2+ efflux in cultured cortical neurons
    Qin Y, Thomas D, Fontaine CP, Colvin RA
    Neurosci Lett. 2009 Jan 30;450(2):206-10
  5. Mechanisms of Zn2+ efflux in cultured cortical neurons
    Qin Y, Thomas D, Fontaine CP, Colvin RA
    J Neurochem. 2008 Dec;107(5):1304-13
  6. Insights into Zn2+ homeostasis in neurons from experimental and modeling studies
    Colvin RA, Bush AI, Volitakis I, Fontaine CP, Thomas D, Kikuchi K, Holmes WR
    Am J Physiol Cell Physiol. 2008 Mar;294(3):C726-42
  7. Tonic Shock Induces Detachment of Giardia lamblia
    Hansen WR, Fletcher DA.
    PLoS Negl Trop Dis. 2008 Feb 13;2(2):e169
  8. The role of Akt in the signaling pathway of the glycoprotein Ib-IX–induced platelet activation
    Yin H, Stojanovic A, Hay N, Du X
    Blood. 2008 Jan 15;111(2):658-65
  9. Endothelial Targeting of High-Affinity Multivalent Polymer Nanocarriers Directed to Intercellular Adhesion Molecule 1
    Silvia Muro, Thomas Dziubla, Weining Qiu, John Leferovich, Xiumin Cui, Erik Berk, and Vladimir R. Muzykantov
    JPET 317:1161–1169, 2006
  10. Acute Glutathione Depletion Restricts Mitochondrial ATP Export in Cerebellar Granule Neurons
    Sabino Vesce1, Mika B. Jekabsons, Linda I. Johnson-Cadwell, and David G. Nicholls
    J. Biol. Chem., Vol. 280, Issue 46, 38720-38728, November 18, 2005

Order No. Model No. Product Description USA Price Order
64-0754 PE-90/10 Polyethylene tubing, PE-90, 10 ft Login Login
64-0379 SA-30UUZ Series 30 stage adapter for Zeiss LSM510 Login Login
64-0380 SA-30UU Series 30 stage adapter for Use with Nikon E400 / E600 / E800, Olympus BX-40 / BX-50 Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories and Replacement Parts
64-1464 RC-30-TCP RC-30 top coverslip plate replacement Login Login
64-1464WA RC-30WA-TCP RC-30WA top coverslip plate replacement Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-1559 RH-3 Replacement heater elements for Series 30 chambers, 2 pack Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
Chambers
64-0321 RC-30HV RC-30HV confocal imaging chamber, with heaters Login Login
64-0321WA RC-30WA RC-30WA wide aperture confocal imaging chamber Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Replacement Gaskets
64-0331 GS-30L/15 Large bath gasket, 375 µm thick, 10 pack Login Login
64-0330 GS-30L/10 Large bath gasket, 250 µm thick, 10 pack Login Login
64-0342 GS-30L/4 Large bath gasket, 125 µm thick, 10 pack Login Login
64-0333 GS-30S/15 Slotted bath gasket, 375 µm thick, 3.2 mm slot, 10 pack Login Login
64-0332 GS-30S/10 Slotted bath gasket, 250 µm thick, 3.2 mm slot, 10 pack Login Login
64-0343 GS-30S/4 Slotted bath gasket, 125 µm thick, 3.2 mm slot, 10 pack Login Login
64-0335 GS-30B/15 Blank gasket, 375 µm thick, 10 pack Login Login
64-0334 GS-30B/10 Blank gasket, 250 µm thick, 10 pack Login Login
64-0341 GS-30B/4 Blank gasket, 125 µm thick, 10 pack Login Login
Series 30 Stage Adapters
64-0322 SA-30NIK Series 30 stage adapter, fits Nikon Diaphot / TE200 / TE300 Login Login
64-0325 SA-30/OLY2 Series 30 stage adapter, fits Olympus IMT2 / IX-50 / IX-70 / BX-50WI and Warner Hybrid Stage Login Login
64-0326 SA-30LZ Series 30 stage adapter, fits Zeiss Axiovert 211 x 230 specimen stage, Leica Dmirbe/E with Plane Stage Login Login
Specimen Stage Compatible
64-0377 SA-30GALVOZ Series 30 stage adapter for Leica Galvo Z Stage Login Login
Standard Dimension Coverslips: #1 Thickness (0.15 ± 0.02 mm)
64-1500 CS-40R Round cover glass, #1 thickness, 40 mm, 50 pack Login Login
64-0706 CS-22/30 Rectangular cover glass, #1 thickness, 22 x 30 mm, 50 pack Login Login
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
Standard Dimension Coverslips: #1.5 Thickness (0.17 ± 0.02 mm)
64-1499 CS-30R15 Round cover glass, #1.5 thickness, 30 mm, 90 pack Login Login

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