Specialized Tools for Electrophysiology and Cell Biology Research

(DS2A, DS3) Constant Voltage / Constant Current Isolated Stimulators

Product Summary

Many experiments in electrophysiology use a brief pulse of voltage or current as a stimulus to excite nerve or muscle cells.

  • Minimal interference with recordings
  • No ground loops
  • DS2A: 100 V maximum at up to 50 mA
  • DS3: 2 µA-32 mA from 90 Volts
  • Battery powered
  • Energy saver circuitry

Many experiments in electrophysiology use a brief voltage or current pulse to excite nerve or muscle cells. The voltage required to send this current through tissues can vary over a wide range. In addition, to minimize artifacts in the experimental results, it is desirable that the source of this E.M.F. should be isolated both from ground and from the timing generator used to release the stimulus.

The DS2A Constant Voltage and DS3 Constant Current isolated stimulators fulfill these requirements by using self contained batteries as the power source and optical coupling is employed to isolate the stimulator from the trigger source. Precise timing of stimulus application can be controlled by a PC or other generating device such as the DG2A Train Delay Generator.

The time duration of these stimulator's output is adjustable from 20 µs - 2 seconds via panel controls or via TTL pulses.

The DS2A has two output ranges with voltage maximums of 9V or 99V, and the DS3 has four overlapping decade ranges providing current maximums of 32 µA, 320 µA, 3.2 mA, and 32 mA. All ranges are continuously adjustable by a calibrated multi-turn output control and the polarity of the output can be reversed by a toggle switch.

 

Current is drawn from the batteries only when delivering an pulse, minimizing the drain on them. The maximum current drain is 30 mA plus the load current. These instruments have nonconductive enclosures and may be mounted on a metal mounting frame without connecting the internal circuitry to ground. A single shot button is provided which can be operated at all times irrespective of the trigger input status.

How do I decide between the constant voltage DS2A and the constant current DS3?

The visible difference between the two units (apart from the color) is that the output of the DS2A is defined in Volts while the output of the DS3 is defined in Amps. The actual stimulus passing through your preparation is measured in current in both cases and is dependent upon Ohms Law (V=IR).

If your preparation has a variable impedance (R) and you are using a constant voltage (V) source such as the DS2A, then the actual current (I) passing through the tissue may vary considerably between each stimulus, which may not be a good idea if you want to apply reproducible stimuli. With the DS3, the constant current circuitry prevents variations in the tissue or electrode impedance from altering the size of the applied current (within the 90 V compliance limit of the unit), leading to the stimulator equivalent of WYSIWYG – “What You Set Is What You Get”.

Unfortunately there are no well defined rules governing the circumstances under which stimulator should be used. Generally speaking, however, the constant voltage stimulator is more common in the literature.

Maximum Voltage Output (DS2A) 99 V (High), 9 V (Low) selected by front panel switch; square wave pulse shape, typical rise time <1 µsec fall time < 3 µsec into resistive load
Current Output Ranges (DS3) 2 to 32 µA, 20 to 320 µA, 0.2 to 3.2 mA and 2 to 32 mA
Output Compliance (DS3) 90 V
Amplitude Control (DS2A & DS3) Multi-turn dial provides continuous adjustment
Output Impedance (DS2A) Dependent on position of amplitude control, 1.25 K max. at center position, 200 Ω at ends of travel
Overload (DS2A) Automatic overload circuit prevents excessive output current being drawn by reducing output pulse width to less than 10 µsec when current of greater than 50 mA is detected
Pulse Width Controls (DS2A & DS3) Two dials providing continuous adjustments over range 20 µsec to 2 sec, as well as selection of input pulse width
1) 6-Position Switch for pulse widths 10 µsec, 100 µsec, 1 msec, 10 msec, 100 msec with ± 10% accuracy or equal to input pulse width
2) Multiplier continuously variable over range x2 to x20; pulse width is product of two dial settings
Trigger Requirements (DS2A & DS3) Positive pulse of min. 3 V, max. 20 V amplitude required to trigger stimulator; trigger input current varies from 7 mA to 63 mA over above voltage range; trigger pulse width should not normally be less than 10 µsec
Trigger Isolation (DS2A & DS3) Optical coupling is employed between trigger source and stimulator circuitry; capacity coupling less than 3 pF
Single Shot (DS2A & DS3) Single shot push button provided
Normal/Reverse Switch (DS2A & DS3) Normal Red output terminal positive
Reverse Red output terminal negative
Batteries Self contained batteries are used to provide isolated source of power; current is drawn only when delivering output pulse; max. current drain is 30 mA plus load current
Battery Type 11 x PP3 - (IEC-6R61) style 9V batteries
Battery Test Sockets Sockets provided on end of instrument case to allow batteries to be checked without removing
Output Terminals Two 2 mm touch proof sockets fitted to front panel for output connections
Dimensions Panel Size 190 x 110 mm (7.5 x 4.3 in)
Depth 80 mm (3.15 in) over knobs
Mounting One or two model DS2A /DS3 stimulators may be mounted in 19 inch rack using D121-11 Mounting Frame
Weight 800 g (28.2 oz) with batteries installed

Order No. Model No. Product Description USA Price Order
65-0329 DS2A-MKII Monopolar stimulator, constant voltage, isolated Login Login
65-0550 DS3 Monopolar stimulator, constant current, isolated Login Login
65-0337 D121-11 Rack mounting frame, 19 in, holds two DS2A/ DS3/ DG2 Login Login
Accessories
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
Field Stimulation Chambers
64-0226 RC-21BRFS Imaging chamber with field stimulation Login Login
64-0226U RC-21BRFS Imaging chamber with field stimulation - polysulfone body Login Login
64-0240R2 RC-27NE2 Narrow Bath Recording Chamber w/ Field Stimulation Login Login
64-0374 RC-47FSLP Quick change chamber for 25 mm coverslips, with field stimulation Login Login
64-1725 RC-49MFSH Magnetic Imaging / recording chamber with removable electrodes and heating elements Login Login

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