Specialized Tools for Electrophysiology and Cell Biology Research

(DH-35) Culture Dish Heater

This resistive heater system enables temperature control of Corning's 35 mm tissue/cell culture dishes and is designed for mounting on most microscopes.

  • Enables controlled heating of Corning 35 mm cell culture dishes
  • Designed for mounting on most microscopes including Nikon, Olympus, Zeiss and Leica
  • 19 mm diameter base viewing aperture
  • Easy dish replacement in the holder
  • Dishes can be placed with or without dish covers
  • Compatible with Warner temperature controllers

The DH-35 Culture Dish Heater is designed to maintain a Corning 35 mm cell culture dish (p/n 25000) at a constant temperature above ambient, usually 37°C. The base of the unit has a 19 mm diameter aperture for unrestrictive viewing of the culture dish and stage adapters are available for mounting onto microscopes from Nikon, Zeiss, Leica and Olympus. See Ordering Information for a listing of specific supported microscopes.

The two resistive heaters in the DH-35 are normally powered using one of Warner's temperature controllers (e.g. the single channel TC-344C or the dual channel TC-334C). Connection from the temperature controller to the DH-35 requires a CC-28 cable assembly. The cable assembly includes the two heater connectors, the control thermistor and an additional thermistor to measure temperature in the dish's bath.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Corning 35 mm cell culture dishes can be placed into the DH-35 with or without their covers. If not using the Corning dish covers, one of the DH-35 dish covers can be used instead. An advantage of the DH-35 cover is that the integral slot allows access to the dish for probes, perfusion tubes, etc. In general, when dishes are used without covers, the dish is secured into the heater using a large O-Ring. The O-Ring fits snugly inside the DH-35 top section.

The DH-35 is constructed from aluminum and has an anodized finish. Cleaning can be performed using common lab detergents or alcohol.

Weight without dish or holder cover 94.4 grams
Weight with one holder cover 98.8 grams
Order No. Model No. Product Description USA Price Order
64-0107 TA-29 Cable with bead thermistor Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login

References

References

  1. Effects of SDPNFLRF-amide (PF1) on voltage-activated currents in Ascaris suum muscle
    Verma S, Robertson AP, Martin RJ
    Int J Parasitol. 2009 Feb;39(3):315-26. Epub 2008 Aug 12
  2. Effects of (S)-amlodipine and (R)-amlodipine on L-type calcium channel current of rat ventricular myocytes and cytosolic calcium of aortic smooth muscle cells
    Wang RX, Jiang WP, Li XR, Lai LH
    Pharmazie. 2008 Jun;63(6):470-4
  3. Ex vivo imaging of motor axon dynamics in murine triangularis sterni explants
    Kerschensteiner M, Reuter MS, Lichtman JW, Misgeld T
    Nat Protoc. 2008;3(10):1645-53
  4. The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle
    Verma S, Robertson AP, Martin RJ
    Br J Pharmacol. 2007 Jul;151(6):888-99. Epub 2007 May 21
  5. Synaptic Vesicle Mobility in Mouse Motor Nerve Terminals with and without Synapsin
    Gaffield MA, Betz WJ
    J Neurosci. 2007 Dec 12;27(50):13691-700
  6. Overexpression of Cytosolic Group IVA Phospholipase A2 Protects Cells from Ca2 -dependent Death
    Casas J, Gijón MA, Vigo AG, Crespo MS, Balsinde J, Balboa MA
    THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 9, pp. 6106–6116, March 3, 2006
  7. Phosphatidylinositol 4,5-Bisphosphate Anchors Cytosolic Group IVA Phospholipase A2 to Perinuclear Membranes and Decreases Its Calcium Requirement for Translocation in Live Cells
    Casas J, Gijón MA, Vigo AG, Crespo MS, Balsinde J, Balboa MA
    Mol Biol Cell. 2006 Jan;17(1):155-62. Epub 2005 Oct 12
  8. Internet-based robotic laser scissors and tweezers microscopy
    Botvinick EL, Berns MW
    Microsc Res Tech. 2005 Oct;68(2):65-74
  9. Preparation of nanoparticles composed of poly(?-glutamic acid)-poly(lactide) block copolymers and evaluation of their uptake by HepG2 cells
    Liang HF, Yang TF, Huang CT, Chen MC, Sung HW
    J Control Release. 2005 Jul 20;105(3):213-25
  10. The lateral mobility of NHE3 on the apical membrane of renal epithelial OK cells is limited by the PDZ domain proteins NHERF1/2, but is dependent on an intact actin cytoskeleton as determined by FRAP
    Cha B, Kenworthy A, Murtazina R, Donowitz M
    J Cell Sci. 2004 Jul 1;117(Pt 15):3353-65
  11. Binding of Filamin to the C-terminal Tail of the Calcitonin Receptor Controls Recycling
    Seck T, Baron R, Horne WC
    J Biol Chem. 2003 Mar 21;278(12):10408-16. Epub 2003 Jan 16
Order No. Model No. Product Description USA Price Order
64-0296 SA-20LZ Series 20 stage adapter for use with Zeiss Axiovert with 211 x 230 Specimen Stage, Leica Leitz DMIL with Object Guide, Zeiss Leitz DMIRBE/E with Plane Stage Login Login
64-0110 DH-35 35 mm culture dish heater Login Login
65-0054 LU-PD Sleeve of 35 mm Petri dishes, 20 pack Login Login
Accessories and Replacement Parts
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0291 SA-NIK Series 20 stage adapter for Nikon Diaphot/TE200/TE300 Login Login
64-0295 SA-OLY/2 Series 20 stage adapter for Olympus IMT2 / IX-50 / IX-70 / IX-80 / IX-81 Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login

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