Specialized Tools for Electrophysiology and Cell Biology Research

RC-26Z Diamond Bath Oocyte Recording Chamber

The RC-26Z, based on the the popular RC-26, is specifically designed to accommodate studies with xenopus oocytes.

  • Designed for TEV oocyte recording
  • Diamond shaped bath provides laminar solution flow
  • Large optical imaging area
  • Can be used with either upright or inverted microscopes
  • Tissue slice anchors available

The RC-26Z is a polycarbonate chamber, similar in design to the RC-26, having two additional features specific for two-electrode oocyte recording: namely, the dam height in the suction well is increased to 2.5 mm to provide an adequate solution level in the bath, and a second agar bridge well has been added to accommodate a bath clamp electrode.

The RC-26Z can also accommodate larger tissue slices and preparations, and permits excellent access for electrodes. The working volume is 170 µl/mm. The chamber floor consists of a 22 x 50 mm coverslip.

The chamber is equipped with a stainless steel suction tube (1.67 mm OD) together with a suction tube clamp. This insures the adjustable suction tube will not move once the height is set by the user.

The RC-26Z chamber requires a platform (PM-1PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

Each RC-26Z is supplied with a package of #1 thickness glass coverslips (CS-22/50, 22x50 mm, 50/pkg) and 10 ft of PE-160 tubing.

Material Polycarbonate
Bath dimensions
(L x W x H)
25 x 12 x 4 mm
Volume, by depth 170 μl/mm
Bottom coverglass 22 x 50 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 

 

Order No. Model No. Product Description USA Price Order
64-0708 CS-22/50 Rectangular cover glass, #1 thickness, 22 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Anxiolytic Effects of Maxipost (BMS-204352) and Retigabine via Activation of Neuronal Kv7 Channels ,
    M. P. G. Korsgaard, B. P. Hartz, W. D. Brown, P. K. Ahring, D. Strøbæk, and N. R. Mirza,
    JPET 314:282-292, 2005
Order No. Model No. Product Description USA Price Order
64-0237 RC-26Z Large bath chamber for oocyte recording, 375 µl volume Login Login
Accessories and Replacement Parts
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-1920 SA-20PS1 Adapter Ring for Pecon Heating Insert P Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Platforms
64-1526 PM-1 PM-1 Platform for Series 20 chambers, heated, magnetic clamps Login Login
64-0277 P-1 P-1 Platform for Series 20 chambers, non-heater Login Login
RC-26 Chamber Slice Anchors
64-0250 SHD-26H/10 Slice anchor for RC-26, 1.0 mm thread spacing, stainless steel Login Login
64-0251 SHD-26H/15 Slice anchor for RC-26, 1.5 mm thread spacing, stainless steel Login Login
64-0252 SHD-26H/2 Slice anchor for RC-26, 2.0 mm thread spacing, stainless steel Login Login
64-0265 SHD-26KIT Slice anchor kit for RC-26, stainless steel Login Login
Standard Dimension Coverslips: #1 Thickness (0.15 ± 0.02 mm)
64-0708 CS-22/50 Rectangular cover glass, #1 thickness, 22 x 50 mm, 50 pack Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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