Specialized Tools for Electrophysiology and Cell Biology Research

(RC-24, RC-24E) Fast Exchange Diamond Bath Chambers

Product Summary

The RC-24 and RC-24E chambers allow for very fast solution exchange for a variety of applications. These recording chambers feature the smallest bath volumes of Warner's open style chambers.

  • Designed for physiological measurements of cell cultured coverslips
  • Applications include patch clamp, imaging, and intracellular/extracellular recordings
  • Diamond shaped bath provides laminar solution flow
  • Both perfusion and aspiration ports on same side of chamber
  • Very small bath volume facilitates very fast solution exchange

The bath volume in these models is among the smallest of the open style chambers. This enables very fast solution exchange for a variety of applications, including patch clamp and electrophysiology studies. The small bath volumes are especially useful when using drugs in small quantities.

The RC-24 and RC-24E have a an unusual characteristic in that both the perfusion inlet and aspiration outlet is on the same side of the chamber. This allows the associated perfusion lines to be conveniently placed on only one side and facilitates electrode placement.

An agar bridge well for reference electrodes is provided, as is an aspiration well. The aspiration well is equipped with a stainless steel aspiration tube together with a clamp. The clamp insures that the adjustable suction tube will not move once the height is set by the user.

The floor of these chambers can be formed from either a 22 mm square coverslip or from a 22 mm x 40 mm rectangular coverslip. Each chamber requires a platform (PM-1PH-1P-1) and a Series 20 stage adapter to complete the assembly for mounting on a microscope.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22x40 mm, 50/pkg) and 10 ft of PE-160 tubing.

  RC-24 RC-24E
Material Polycarbonate Polycarbonate
Bath dimension
(L x W x H)
15 x 5 x 3.8 mm 15 x 6 x 3.8 mm
Volume, by depth 40 μl/mm 50 μl/mm
Bottom coverglass 22 x 40 mm 22 x 40 mm
Input tubing (ID x OD) 1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)

 

 

Order No. Model No. Product Description USA Price Order
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0704 CS-22S Square cover glass, #1 thickness, 22 x 22 mm, 100 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. The Effect of Thermal Acclimation on Action Potentials and Sarcolemmal K+ Channels from Pacific Bluefin Tuna Cardiomyocytes
    Galli GL, Lipnick MS, Block BA
    Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R502-9. Epub 2009 Jun 10
  2. Analogs of MK-499 are differentially affected by a mutation in the S6 domain of the hERG K+ channel
    Karczewski J, Wang J, Kane SA, Kiss L, Koblan KS, Culberson JC, Spencer RH
    Biochem Pharmacol. 2009 May 15;77(10):1602-11. Epub 2009 Feb 27
  3. Pharmacological properties of a pore induced by raising intracellular Ca2+
    Faria RX, Reis RA, Casabulho CM, Alberto AV, de Farias FP, Henriques-Pons A, Alves LA
    Am J Physiol Cell Physiol. 2009 Jul;297(1):C28-42. Epub 2009 Mar 25
  4. D1 dopaminergic control of G protein–dependent inward rectifier K+ (GIRK)–like channel current in pyramidal neurons of the medial prefrontal cortex
    Witkowski G, Szulczyk B, Rola R, Szulczyk P
    Neuroscience. 2008 Jul 31;155(1):53-63. Epub 2008 Jun 19
  5. Modulation of Ca2+ channel current by mu opioid receptors in prefrontal cortex pyramidal neurons in rats
    Rola R, Jarkiewicz M, Szulczyk P
    Acta Neurobiol Exp (Wars). 2008;68(1):10-25
  6. High [Na+]i in cardiomyocytes from rainbow trout
    Birkedal R, Shiels HA
    Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R861-6. Epub 2007 May 16
  7. Decreased Neuronal Death in Na+/H+ Exchanger Isoform 1-Null Mice after In Vitro and In Vivo Ischemia
    Jing Luo, Hai Chen, Douglas B. Kintner, Gary E. Shull, and Dandan Sun
    The Journal of Neuroscience, December 7, 2005, 25(49):11256-11268
  8. Orexin-A-induced Ca2+ Entry EVIDENCE FOR INVOLVEMENT OF TRPC CHANNELS AND PROTEIN KINASE C REGULATION*,
    Kim P. Larsson, Hanna M. Peltonen, Genevieve Bart, Lauri M. Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P. Kukkonen, and Karl E. O. Åkerman,
    J. Biol. Chem., Vol. 280, Issue 3, 1771-1781, January 21, 2005
  9. Stimulation of astrocyte Na+/H+ exchange activity in response to in vitro ischemia depends in part on activation of ERK1/2
    Douglas B. Kintner, Andy Look, Gary E. Shull, and Dandan Sun
    Am J Physiol Cell Physiol 289: C934-C945, 2005
  10. The contribution of ionic currents to changes in refractoriness of human atrial myocytes associated with chronic atrial fibrillation
    Workman AJ, Kane KA, Rankin AC
    Cardiovasc Res. 2001 Nov;52(2):226-35

Order No. Model No. Product Description USA Price Order
Chambers
64-0230 RC-24 Fast exchange recording chamber, 40 µl volume Login Login
64-0231 RC-24E Fast exchange recording chamber, 50 µl volume Login Login
Platforms
64-1526 PM-1 PM-1 Platform for Series 20 chambers, heated, magnetic clamps Login Login
64-0284 PH-1 PH-1 Platform for Series 20 chambers, heater Login Login
64-0277 P-1 P-1 Platform for Series 20 chambers, non-heater Login Login
Replacement Consumables
64-0704 CS-22S Square cover glass, #1 thickness, 22 x 22 mm, 100 pack Login Login
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Replacement Parts
64-1431 ST-1S Suction Tube, Series 20 Classic Design, straight style Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
64-1920 SA-20PS1 Adapter Ring for Pecon Heating Insert P Login Login

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