Specialized Tools for Electrophysiology and Cell Biology Research

(BSC-HT) Haas Top

 

The BSC-HT Haas Top is an interface-type chamber utilizing semi-submersion principles for the study of brain/tissue slices.

  • Maintains a stable intracellular membrane potential of more than 60 mV
  • Facilitates action potentials with overshoots close to 100 mV in amplitude, duration of less than 2 msec at 32°C, when used with hippocampal pyramidal and granular cells
  • Maintenance of the above potentials is possible for more than 1 hour undisturbed by changes in perfusion fluids
  • Preparation slices remain viable for up to 12 hours
  • Using flow rates of 1 ml/min, will completely exchange the chamber fluid in less than 1 minute

The Haas Top is an interface-type chamber utilizing semi-submersion principles for the study of brain/tissue slices.

  • Maintains a stable intracellular membrane potential of more than 60 mV
  • Facilitates action potentials with overshoots close to 100 mV in amplitude, duration of less than 2 msec at 32°C, when used with hippocampal pyramidal and granular cells
  • Maintenance of the above potentials is possible for more than 1 hour undisturbed by changes in perfusion fluids
  • Preparation slices remain viable for up to 12 hours
  • Using flow rates of 1 ml/min, will completely exchange the chamber fluid in less than 1 minute

The Haas Top (BSC-HT) provides for rapid exchange of superfusing fluids, and excellent stability for intracellular recording. Normally used in conjunction with the BSC-BUW or BSC-BU base units and a temperature controller. Slices rest on nylon mesh below which a thin sheet of the perfusate of interest flows. The bottom side of the slice is wet as capillary action saturates the mesh. Slices are oxygenated by a warmed and humidified mixture of 95/5% O2/CO2 which flows across the top surface of the slice. The BSC-HT has been designed so that the effects of two drugs can be studied simultaneously. One drug flows down the left portion of the chamber and the second down the right.

Dimensions
(L x W x H)
111.13 x 85.41 x 23.80 mm
(4.375 x 3.365 x 0.937 in)
Weight 130.41 g (4.60 oz)

References

  1. White Matter Axon Vulnerability to AMPA/Kainate Receptor-Mediated Ischemic Injury Is Developmentally Regulated
    William J. McCarran, and Mark P. Goldberg
    The Journal of Neuroscience, April 11, 2007, 27(15):4220-4229; doi:10.1523/JNEUROSCI.5542-06.2007
  2. GABA Receptor-Mediated Inhibition of Neuronal Activity in Rat SCN In Vitro: Pharmacology and Influence of Circadian Phase
    Valentin K. Gribkoff, Rick L. Pieschl and F. Edward Dudek
    J Neurophysiol 90: 1438-1448, 2003. First published May 15, 2003; doi:10.1152/jn.01082.2002
  3. AMPA/Kainate Receptor Activation Mediates Hypoxic Oligodendrocyte Death and Axonal Injury in Cerebral White Matter
    Selva Baltan Tekkök and Mark P. Goldberg 
    The Journal of Neuroscience, June 15, 2001, 21(12):4237-4248
Order No. Model No. Product Description USA Price Order
65-0078 BSC-AIR Aeration Element Replacement, Set of 2 Login Login
65-0081 BSC-TUBE Base Unit (BSC-BU) tubing set replacement, Tygon 0.031 x 0.093 (ID x OD) Login Login
65-0079 BSC-ORING Base Unit (BSC-BU) replacement gasket set Login Login
65-0082 BSC-G10 Haas top (BSC-HT) tissue insert replacement, Set of 2 Login Login
65-0084 BSC-HT-AG Haas top (BSC-HT) ground wire replacement Login Login
65-0608 BSC-PC Insert Insert for BSC-PC Prechamber Tissue Slice Chamber Login Login
65-0090 BSC-MESH Replacement mesh for Haas and Zbicz tops, and for prechamber cup bottom, 4 x 4 in Login Login
65-0088 BSC-ACC-KIT Base Unit, Haas and Zbicz tops accessory kit (replacement screws, fittings and mesh) Login Login
Accessories
65-0045 TC-202A Med Systems bipolar temperature controller compatible with PDMI-2, PSMI, CSMI, LU-CB-1, LU-CPC-CEH, and BSC-BU Login Login
Accessories and Replacement Parts (General)
65-0083 BSC-HTC Haas top (BSC-HT) plastic cover replacement Login Login
65-0086 BSC-ZTC Zbicz Top (BSC-ZT) plastic insert replacement Login Login
65-0087 BSC-PC-VAL Prechamber (BSC-PC) valve replacement Login Login
Accessories and Replacement Parts (Med Systems)
65-0080 BSC-HE Replacement heating wire for BSC-BU Login Login
65-0091 BSC-CABLE BSC-BU connecting cable for Med Systems temperature controllers Login Login
65-0104 Output cable, 6 pin to banana for TC-202A or BSC-BU Login Login
Accessories and Replacement Parts (Warner)
64-1697 BSC-HEW Replacement heating wire for BSC-BUW Login Login
64-1591 BSC-CABLE-W BSC-BUW connecting cable for Warner temperature controllers Login Login
Heater Controllers
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
Med Systems Based System
65-0073 BSC-BU BSC-BU base unit compatible with Med Systems temperature controllers, Includes tubing, heating element and electrical terminals Login Login
Warner Based System
64-1525 BSC-BUW BSC-BU base unit compatible with Warner temperature controllers, includes connecting cable Login Login
65-0074 BSC-ZT Zbicz Top including nylon mesh Login Login
65-0075 BSC-HT Haas top including 2 slice holders Login Login
65-0076 BSC-PC Prechamber for holding slices Login Login

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