Specialized Tools for Electrophysiology and Cell Biology Research

(RC-21B, RC-21BR) Large Closed Bath Imaging Chambers

Polycarbonate chambers providing a large viewing area and good access for microscope objectives.

  • Closed bath chamber design
  • Diamond fluidics
  • Ideal for fluorescent, calcium, and time-lapse imaging studies
  • Uses 22 mm square or 25 mm round coverslips
  • Large optical imaging area
  • Good access for upright or inverted microscopes

The RC-21B and RC-21BR chambers feature a closed bath, short working distances, and linear solution flow. Distance between the top and bottom coverslip is 2.5 mm and total bath volume is 358 µl.

At the bath output, a length of tubing is used as a back pressure control to insure smooth solution flow. An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe. This port is useful for injecting substances or for removing air bubbles from the sealed chamber.

Studies involving physiological bicarbonate buffers can be performed with these chambers with no gas loss at the chamber interface. The closed design allows the use of permeable supports. With laminar flow, minimal distortion of the sample occurs during perfusion.

RC-21B and RC-21BR chambers require a platform (PM-2PH-2 or P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22S or CS-25R, respectively, 100/pkg) and 10 ft of PE-160 polyethylene tubing.

  RC-21B RC-21BR
Material polycarbonate polycarbonate
Bath dimensions
(L x W x H)
17 x 13 x 2.5 mm 17 x 13 x 2.5 mm
Outer dimensions
(L x W x H)
47.5mm x 28.5mm x 2.5mm 47.5mm x 28.5mm x 2.5mm
Volume 260 μl 260 μl
Top coverglass 22 mm square 25 mm round
Bottom coverglass 22 mm square 25 mm round
Injection port
(ID x OD)
0.91 x 0.56 mm, stainless steel 0.91 x 0.56 mm, stainless steel
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)

 

 

 

 

Order No. Model No. Product Description USA Price Order
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0704 CS-22S Square cover glass, #1 thickness, 22 mm, 100 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
    Kwinter D, Michael S
    J Vis Exp. 2009 May 29;(27). pii: 1144. doi: 10.3791/1144
  2. Monitoring the in vivo redox state of plant mitochondria: Effect of respiratory inhibitors, abiotic stress and assessment of recovery from oxidative challenge
    Schwarzländer M, Fricker MD, Sweetlove LJ
    Biochim Biophys Acta. 2009 May;1787(5):468-75. Epub 2009 Feb 3
  3. Influence of the Dissolution Rate on the Collapse and Shedding Behavior of Monostearin/Monopalmitin-rich Coated Microbubbles
    Shen Y, Powell RL, Longo ML
    Langmuir. 2008 Sep 16;24(18):10035-40. Epub 2008 Aug 22
  4. Confocal imaging of glutathione redox potential in living plant cells
    Schwarzländer M, Fricker MD, Müller C, Marty L, Brach T, Novak J, Sweetlove LJ, Hell R, Meyer AJ
    J Microsc. 2008 Aug;231(2):299-316
  5. Intracellular pH Homeostasis Plays a Role in the Tolerance of Debaryomyces hansenii and Candida zeylanoides to Acidified Nitrite
    Mortensen HD, Jacobsen T, Koch AG, Arneborg N
    Appl Environ Microbiol. 2008 Aug;74(15):4835-40. Epub 2008 Jun 6
  6. Characterizing molecular probes for diffusion measurements in the brain
    Kaur G, Hrabetova S, Guilfoyle DN, Nicholson C, Hrabe J
    J Neurosci Methods. 2008 Jun 30;171(2):218-25. Epub 2008 Mar 28
  7. Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
    Anggono V, Cousin MA, Robinson PJ
    Methods Mol Biol. 2008;457:333-45
  8. A Fluorescent Sensor for Imaging Reversible Redox Cycles in Living Cells
    Evan W. Miller, Shelly X. Bian, Christopher J. Chang
    J Am Chem Soc, 2007 - pubs.acs.org
  9. NHE1 inhibition by amiloride- and benzoylguanidine-type compounds. Inhibitor binding loci deduced from chimeras of NHE1 homologues with endogenous differences in inhibitor sensitivity
    Pedersen SF, King SA, Nygaard EB, Rigor RR, Cala PM
    J Biol Chem. 2007 Jul 6;282(27):19716-27. Epub 2007 May 10
  10. The Polypeptide Composition of Moving and Stationary Neurofilaments in Cultured Sympathetic Neurons
    Yan Y, Jensen K, Brown A
    Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309
  11. D-box is required for the degradation of human Shugoshin and chromosome alignment
    Fu G, Hua S, Ward T, Ding X, Yang Y, Guo Z, Yao X
    Biochem Biophys Res Commun. 2007 Jun 8;357(3):672-8. Epub 2007 Apr 12
  12. Chemical inhibition of the Pho85 cyclin-dependent kinase reveals a role in the environmental stress response
    Carroll AS, Bishop AC, DeRisi JL, Shokat KM, O'Shea EK
    Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12578-83

Order No. Model No. Product Description USA Price Order
64-1561 PM-2 PM-2 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories and Replacement Parts
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Chambers
64-0224 RC-21B Large bath chamber, 260 µl volume, 22 mm square coverslip Login Login
64-0225 RC-21BR Large bath chamber, 260 µl volume, 25 mm round coverslip Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Platforms With Heating Elements
64-0285 PH-2 PH-2 Platform for Series 20 chambers, heater Login Login
Platforms Without Heating Elements
64-0278 P-2 P-2 Platform for Series 20 chambers, non-heater Login Login
Standard Dimension Coverslips: #1 Thickness (0.15 ± 0.02 mm)
64-0704 CS-22S Square cover glass, #1 thickness, 22 mm, 100 pack Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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