Specialized Tools for Electrophysiology and Cell Biology Research

(RC-31A) Low Profile Parallel Plate Flow Chamber

Product Summary

The RC-31A is a closed bath, low profile chamber incorporating special features for microscopic confocal imaging.

 

  • Adjustable distance between upper and lower coverslips, 125 µm to 375 µm
  • Optimal control of culture conditions and cellular environment
  • Compatible with both upright and inverted microscopes
  • Amenable for long term studies using live cells
  • Uses Warner's Series 30 stage adapters

Unique features of the RC-31A include user defined bath geometry and a large viewing area. A 30 mm, #1.5 glass coverslip forms the top of the chamber, while a 40 mm, #1.5 coverslip forms the bottom, respectively, creating a closed bath. The closed design permits cells to be cultured on either the top or bottom coverslips allowing the chamber to be used with either inverted or upright microscopes. The final viewing aperture is 25 mm diameter.

Variable Bath Volume/Fast Perfusate Exchange
The side walls of the RC-31A bath is formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 125-1000 µm and is defined by the thickness and number of gaskets used.

 

Gaskets
Pre-cut gaskets having two different geometries are also supplied; one having a wide cut to allow full use of the 17.7 mm diameter aperture window, and a narrow, slotted version (4 x 37 mm) designed for fast solution exchange. Blank gaskets are also supplied with the chamber permitting the development of the customized bath geometry required for individual applications.

The RC-31A is fully compatible with Warner's single channel (TC-324C) or dual channel (TC-344C) temperature controllers.

 

The RC-31A comes complete with 30 mm round top coverglass, 40 mm round bottom coverglass, blank gaskets (10 pk each in 125, 250, and 375 µm thickness), slotted gaskets (10 pk each in 125, 250, and 375 µm thickness), and wide gaskets (10 pk each in 125, 250, and 375 µm thickness), and 10 ft PE-90 Tubing

Physical Dimensions (H X D) 11.5 x 82 mm
Viewing Area 23 mm in diameter
Bath volumes For 250 um thick gaskets:
Slotted bath 26 µl
Wide bath 133 µl
Weight 0.22 kg assembled
Construction Aluminum, black anodized
Gaskets Silicone rubber
Input/Output Tubing PE-90
Coverslips Top: 30 mm round, #1.5 Thickness
Bottom: 40 mm round, #1.5 Thickness
Microscope Stage Adapters Uses Warner Series 30 stage adapters
 
Order No. Model No. Product Description USA Price Order
64-0330 GS-30L/10 Large bath gasket, 250 µm thick, 10 pack Login Login
64-0331 GS-30L/15 Large bath gasket, 375 µm thick, 10 pack Login Login
64-0332 GS-30S/10 Slotted bath gasket, 250 µm thick, 3.2 mm slot, 10 pack Login Login
64-0333 GS-30S/15 Slotted bath gasket, 375 µm thick, 3.2 mm slot, 10 pack Login Login
64-0334 GS-30B/10 Blank gasket, 250 µm thick, 10 pack Login Login
64-0335 GS-30B/15 Blank gasket, 375 µm thick, 10 pack Login Login
64-1499 CS-30R15 Round cover glass, #1.5 thickness, 30 mm, 90 pack Login Login
64-1696 CS-40R15 Round cover glass, #1.5 thickness, 40 mm, 100 pack Login Login
64-0342 GS-30L/4 Large bath gasket, 125 µm thick, 10 pack Login Login
64-0341 GS-30B/4 Blank gasket, 125 µm thick, 10 pack Login Login
64-0343 GS-30S/4 Slotted bath gasket, 125 µm thick, 3.2 mm slot, 10 pack Login Login

Order No. Model No. Product Description USA Price Order
64-1685 RC-31A Confocal imaging chamber (uses Series 30 platforms) Login Login
Replacement Gaskets
64-0342 GS-30L/4 Large bath gasket, 125 µm thick, 10 pack Login Login
64-0330 GS-30L/10 Large bath gasket, 250 µm thick, 10 pack Login Login
64-0331 GS-30L/15 Large bath gasket, 375 µm thick, 10 pack Login Login
64-0343 GS-30S/4 Slotted bath gasket, 125 µm thick, 3.2 mm slot, 10 pack Login Login
64-0332 GS-30S/10 Slotted bath gasket, 250 µm thick, 3.2 mm slot, 10 pack Login Login
64-0333 GS-30S/15 Slotted bath gasket, 375 µm thick, 3.2 mm slot, 10 pack Login Login
64-0341 GS-30B/4 Blank gasket, 125 µm thick, 10 pack Login Login
64-0334 GS-30B/10 Blank gasket, 250 µm thick, 10 pack Login Login
64-0335 GS-30B/15 Blank gasket, 375 µm thick, 10 pack Login Login
Replacement Consumables
64-1696 CS-40R15 Round cover glass, #1.5 thickness, 40 mm, 100 pack Login Login
64-1499 CS-30R15 Round cover glass, #1.5 thickness, 30 mm, 90 pack Login Login
64-0754 PE-90/10 Polyethylene tubing, PE-90, 10 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login

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