(RC-31A) Low Profile Parallel Plate Flow Chamber

Unique features of the RC-31A include user defined bath geometry and a large viewing area. A 30 mm, #1.5 glass coverslip forms the top of the chamber, while a 40 mm, #1.5 coverslip forms the bottom, respectively, creating a closed bath. The closed design permits cells to be cultured on either the top or bottom coverslips allowing the chamber to be used with either inverted or upright microscopes. The final viewing aperture is 25 mm diameter.

Variable Bath Volume/Fast Perfusate Exchange
The side walls of the RC-31A bath is formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 125-1000 µm and is defined by the thickness and number of gaskets used.

Gaskets
Pre-cut gaskets having two different geometries are also supplied; one having a wide cut to allow full use of the 17.7 mm diameter aperture window, and a narrow, slotted version (4 x 37 mm) designed for fast solution exchange. Blank gaskets are also supplied with the chamber permitting the development of the customized bath geometry required for individual applications.

The RC-31A is fully compatible with Warner's single channel (TC-324C) or dual channel (TC-344C) temperature controllers.