Specialized Tools for Electrophysiology and Cell Biology Research

(PDMI-2) Med Systems Open Perfusion Microincubator

Product Summary

This microincubation system operates equally well under both static and continuously perfused situations.

  • Uses Peltier technology
  • Enables intracellular, whole cell or single channel patch clamp recordings from dissociated cells, cultured cells, and brain slice preparations
  • Enables long-term optical examination of living tissue
  • Heats or cools both media and gas
  • Operating temperature ranges from ~5 to 50 °C

When operated with perfusion, the PDMI-2 microincubator has unique design places flexible tubes carrying inflowing perfusate in contact with a temperature regulated Peltier heat pump driven plate, thus heating or cooling the incoming fluid to a command temperature. This eliminates the need for auxiliary preheating or cooling. The Peltier plate also heats or cools the central chamber containing the cells uniformly when no perfusate is flowing.

Flexible Temperature Range
The operating temperature range of the PDMI-2 Open Perfusion Microincubator is nominally 10-14 °C below ambient to 50 °C when used with the TC-202A Temperature Controller but without supplementary water cooling of its fins.

Temperatures as low as 5°C can be achieved by using an optional water carrying Cu coil which attaches to the perimeter fins of the PDMI-2. Temperatures stabilize quickly throughout the operating range and temperature uniformity across the chamber is excellent.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Optimal and Flexible Perfusion
Different modes of operation are possible:

  • Static chamber medium at a constant set temperature
  • Continuous perfusion at a constant set temperature
  • Switching perfusion of extracellular medium at a set temperature
  • Discontinuous perfusion
  • Rapid temperature changes (with perfusion)

Two temperature controlled perfusion lines deliver perfusate solutions through the microincubator into the chamber a nominal rate of 3 ml per minute each. Fluid level is maintained by placing an aspirator (model LU-ASP) in the chamber.

Fluid height is easily adjusted via a simple screw mechanism. A cleverly designed suction port eliminates fluid flutter thus making for quiet aspiration. A magnetic base secures the aspirator to the microincubator at a user selectable convenient location.

Excellent pH Control
The PDMI-2 also provides gas flow over the top of the chamber for pH control and improved temperature uniformity. The open design provides excellent mechanical and optical access to the media.

Low Noise Conditions
A PTFE well is provided with an Ag-AgCl electrode at its base to allow for the formation of a salt bridge when making low noise electrophysiological recordings.

Peltier Device Current Rating Maximum of 6 amperes continuous at ~3 VDC
Temperature Range

When mounted on a microscope stage the operating temperature ranges from 10-14 °C below ambient to 50 °C using the TC-202A bipolar temperature controller but without any supplementary water cooling of heat fins.

Under the same conditions one can achieve as low as 5°C to 50°C using the TC-202A but also supplying water cooling using an optional Cu coil placed in the fins of the PDMI-2.

Temperature Rate of Change For a change of ~10° from 22° (sic. ambient) to 32°C with perfusion flow rates ranging from 1-3 mL/min requires about 3 minutes, using the TC-202A bipolar controller. Slower rates (i.e. longer times) would be obtained using a simple VDC supply.
Media Perfusion Flow Rate Up to 3.0 mL/min under normal conditions. Faster rates are possible if the internal tubing is replaced with either a longer pieces and/or a thinner wall thickness.
Thermal Expansion ± 4 µm between 15° to 30°C
± 20 µm between 30° to 40°C
Plastic Perfusate Tubes PTFE tubing
Chamber Options Corning 35 mm Petri dishes, LU-CSD, MSC-TD or MSC-PTD
Recommended Gas Flow 0.5 to 2.0 L/min
Temperature Stability ± 0.2°C with TC-202A, at 37°C with 1 ml/min perfusion
Temperature Gradient 2°C with 1.5 ml media volume and 1 ml/min perfusion
Temperature Sensor Thermistor: 100 kΩat 25°C - YSI 44011
Dimensions, H x D 17 x 152 mm (0.67 x 6.0 in)
Weight 0.5 kg (17.9 oz)
Microscope Stage Mounting Call for options
Order No. Model No. Product Description USA Price Order
65-0045 TC-202A Med Systems bipolar temperature controller compatible with PDMI-2, PSMI, CSMI, LU-CB-1, LU-CPC-CEH, and BSC-BU Login Login
65-0058 PDMI-TUBE PDMI-2 copper cooling tube coil (shaped to conform to fin channel) with open ends Login Login
64-2342 GHS-250 Gas humidification system Login Login
65-0093 PDMI-ARL Alignment ring for Leica microscope, 8 x 1.8 mm (OD x thickness) Login Login
65-0059 PDMI-ARN PDMI-2/PSMI alignment ring for Nikon Diaphot (Old or 300/200) TMD Stage, 4.242 x .071 in (D x Thickness) Login Login
65-0060 PDMI-ARO PDMI-2/PSMI alignment ring for Olympus IX-50/70 or IMT2 Fixed Stage, 4.320 x .071 in (D x Thickness) Login Login
65-0061 PDMI-ARZ PDMI-2/PSMI alignment ring for Zeiss Axiovert Gliding or Rotary Stages, 4.046 x .071 in (D x Thickness) Login Login

Order No. Model No. Product Description USA Price Order
65-0066 D-ORING-1 Replacement cover slip dish o-rings for LU-CSD or MS-CTD, small (23.80 mm OD) and large (29.80 mm OD) (2) Login Login
65-0093 PDMI-ARL Alignment ring for Leica microscope, 8 x 1.8 mm (OD x thickness) Login Login
65-0070 MI-T Replacement perfusion/aspiration PTFE tubing for PDMI-2 or PSMI, 2 ft L x 26 AWG (8) Login Login
65-0061 PDMI-ARZ PDMI-2/PSMI alignment ring for Zeiss Axiovert Gliding or Rotary Stages, 4.046 x .071 in (D x Thickness) Login Login
98-2165 PDMI-2-K Open perfusion microincubator, comes with LU-PD and LU-ASP (fits Zeiss K Stage) Login Login
65-0058 PDMI-TUBE PDMI-2 copper cooling tube coil (shaped to conform to fin channel) with open ends Login Login
65-0057 BSC-T3 Bath thermistor for use with PDMI-2, PSMI, LU-CB-1, and TC-202A Login Login
65-0016 BSC-T2A Replacement bath thermistor for use with LU-CPC-CEH and TC-102 Login Login
65-0050 MSC-PTD PTFE Glass Cover Slip Dish; 35 mm D, 9.5 x 19 mm (W x L) Rectangular Optical Window Login Login
65-0055 BSC-TH Magnetic thermistor holder for LU-CB-1 thermistors Login Login
65-0047 LU-ASP Leiden aspirator, cover slip dish fluid level setting device, 38 mm H Login Login
65-0069 CS-GLASS-2 Glass cover slips (#2) for LU-CSD or MSC-PTD, 0.180.25 mm thickness, 25 mm OD, 100 pack Login Login
65-0060 PDMI-ARO PDMI-2/PSMI alignment ring for Olympus IX-50/70 or IMT2 Fixed Stage, 4.320 x .071 in (D x Thickness) Login Login
65-0072 TC-CA Replacement output cable to connect TC-102 Temperature Controller to LU-CB-1 or LU-CPC-CEH Login Login
65-0063 PDMI-FPZ PDMI-2/PSMI fixing platform for Zeiss Axiovert with attachable mechanical stage, MType Stage Login Login
65-0043 PDMI-2 Open perfusion microincubator, comes with LU-PD and LU-ASP (microscope stage adapter not included) Login Login
65-0065 LU-FPZ LU-CB-1 fixing platform for Zeiss Axiovert with attachable mechanical stage, mtype stage Login Login
65-0098 BSC-T2 Replacement T2 bath thermistor for obsolete TC-102 Login Login
65-0049 LU-CSD-Special Leiden multi well cover slip dish; 35 mm D PTFE dish with two (2) kidney-shaped inner optical window wells Login Login
65-0052 MI-M Mini magnets for top surface of microincubators (LU-CB-1, PDMI-2 or PSMI), set of 4 Login Login
65-0054 LU-PD Sleeve of 35 mm Petri dishes, 20 pack Login Login
65-0067 D-ORING-2 Replacement cover slip dish o-rings for MSC-PTD, small (20.80 mm OD) and large (29.80 mm OD) (2) Login Login
65-0059 PDMI-ARN PDMI-2/PSMI alignment ring for Nikon Diaphot (Old or 300/200) TMD Stage, 4.242 x .071 in (D x Thickness) Login Login
65-0071 LU-CPC-P Silicone rubber plugs for LU-CPC or LU-CPC-CEH (4) Login Login
65-0062 PDMI-FPL PDMI-2/PSMI fixing platform for Leica DAS Mikroskop DMIL and DMIRB/E with attachable mechanical Stage Login Login
65-0064 LU-FPL LU-CB-1 fixing platform for Leica DAS Mikroskop DMIL and DMIRB/E with attachable mechanical stage Login Login
65-0056 BSC-T3A Bath thermistor for use with LU-CPC-CEH and TC-202A Login Login
65-0047 LU-ASP Leiden aspirator, cover slip dish fluid level setting device, 38 mm H Login Login
65-0051 MSC-TD PTFE Glass Cover Slip Dish; 35 mm D with removable handle, 35 mm D PTFE dish, 19 mm D optical window Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
Accessories
65-0058 PDMI-TUBE PDMI-2 copper cooling tube coil (shaped to conform to fin channel) with open ends Login Login
64-2342 GHS-250 Gas humidification system Login Login
65-0045 TC-202A Med Systems bipolar temperature controller compatible with PDMI-2, PSMI, CSMI, LU-CB-1, LU-CPC-CEH, and BSC-BU Login Login
Valve Control Systems
64-1940 DWV Dedicated Workstation Vacuum Login Login
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login

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