Specialized Tools for Electrophysiology and Cell Biology Research

(OC-725D) Oocyte Voltage Clamp

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Product Summary

Designed for Two-electrode whole-cell voltage clamping of Xenopus oocytes 

 

  • High compliance voltage (±180 V)
  • Unique bath clamp circuitry
  • Ideal for clamping large cells and cell structures
    e.g. squid axons
  • Extended current measuring range
  • Decreased noise level and 4-pole Bessel filter
  • Voltage and current electrode holders must be purchased separately

The OC-725D Oocyte Clamp is designed for two-electrode, whole-cell voltage clamping of Xenopus oocytes. The dedicated design of the OC-725D includes such features as high compliance voltage (±180 V) and unique bath clamp circuitry. These same features also make the OC-725D ideal for clamping other large cells and cell structures such as squid axons. Improvements to this version include a 4-pole Bessel filter on the I Monitor output with a range of 50 Hz to 10 kHz, or full bandwidth. The electronics have been redesigned to improve efficiency and reliability.

Fast Stable Voltage Clamping

The OC-725D combines high AC and DC gains, and a voltage compliance of ±180 volts to insure fast, non-saturating clamp performance under nearly any condition. The AC clamp gain is variable up to 2000. An additional DC gain of 1 x 106 may be employed for high conductance cells (leaky oocytes).

Two clamp speeds are available: The Slow mode is used for screening oocytes or for applications not requiring fast response times. The Fast mode is used for accurate voltage clamp of fast whole cell currents. Clamp response time in the Fast mode is 550 µsec (10-90% rise time) when applying a 100 mV step to the included model cell*.

Improved Bath Clamp Headstage

The current measuring range of the OC-725D bath clamp headstage has been extended at both ends by the addition of a 3 position range multiplier. Smaller currents are amplified to usable levels and larger currents up to 1 mA can be recorded without output saturation. The unique design of the bath clamp eliminates the need for series resistance compensation. It provides an accurate measurement of bath current by creating a virtual ground in the bath while simultaneously clamping the bath potential at zero.

 OC-725 bath clamp headstage

Voltage Headstage Probe

The voltage measuring headstage is a single-ended, high-impedance probe. Its small size, convenient mounting rod, and 2 meter cable make for easy attachment to a micropositioner. The headstage input is a 2 mm diameter pin. An electrode holder with a 2 mm jack (supplied) mounts directly on the headstage.

 OC-725D voltage probe

Voltage & Current Meters

Independent meters provide simultaneous displays of membrane voltage Vm and membrane current Im. To assure proper impalement of the current electrode, the current meter displays membrane potential Ve from the current electrode before the clamp circuit is turned on.

Clamp Commands

The internal Hold control is a ten turn potentiometer with two ranges; ±0 to 100 mV (x10) and ±0 to 200 mV (x20). External command signals applied to Command IN ÷10 are attenuated to reduce noise from the command source. Hold and external commands are summed.

Additional Features

  • Buzz controls (1 kHz square wave) for each electrode aid in penetration of cell membranes with a minimum of leakage.
  • Overload alarm (audible and visual) indicate when the compliance voltage is exceeded safeguarding the oocyte and indicating that current records are subject to saturation.
  • DC Offsets for both voltage and current electrodes.
  • Electrode Test for both electrodes.

*Model cell: 1 MΩ in parallel with 500nF, 1 MΩ current and voltage electrodes.

 OC-725D model cell

Electrode Holders

Two vented electrode holders with silver wires are typically required with the clamp; a straight type for use with the voltage headstage and a 45° type with mounting handle for use with current electrodes. Vents have been added to the electrodes to prevent pressure build-up inside the electrode which can damage oocytes. A two meter length cable assembly is provided to connect the current electrode holder to the clamp.

Supplied Hardware

The OC-725D is supplied with:

  • 7250V voltage headstage
  • 7251I bath clamp headstage
  • 7259C current cable
  • Model cell, rack mount hardware

Purchased separately: One straight and one 45º electrode holder is also needed and must be purchased separately

Test Condition #1 Model cell used to obtain specifications.
Model membrane: 1 MΩ in parallel with 550 nF.
Current and voltage electrodes: both 1 MΩ impedance.
Test Condition #2 Noise measurements made with an 8-pole Bessel filter.
Voltage Recording Channel (Vm) V Probe Input Impedance 0.5 x 1012Ω, 1 pF
Output Resistance 100 Ω
DC Offset ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn).
Noise (0-10 kHz) 3 µV RMS with input grounded
20 µV RMS with model cell
Electrode Test 10 mV/MΩ read on meter
100 mV/MΩ at Vm x10 output
Meter Range ± 199.9 mV full scale
Current Sensing Channel (Bath Clamp) (Im) Noise (0-1 kHz) 4.5 nA RMS with bath clamp
10 nA RMS in output leg
I Monitor Output 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10.
Gain Telegraph Output 0.2 to 2.8 V in 8 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10.
Compatible with data acquisition software
Meter Range, full scale Clamp Current: ± 199.9 µA
Electrode Voltage (Ve) ± 199.9 mV; current meter reads Ve when clamp mode switch is off.
Current Electrode Channel Compliance Voltage ± 180 V
Clamp Speed 550 µsec; (10-90%) with 100 mV square wave command applied to model cell
Gain Variable AC/DC: 0 to 2000
Fixed DC Gain Switch selected: 1 x 106
Ve DC Offset ± 200 mV at input (20 mV/turn)
Electrode Test 10 mV/MΩ read on current meter
100 mV/MΩ at Ve x10 output rear panel
Commands Hold Manually set with digital potentiometer, 2 digit resolution and 2 ranges:
x10 range: 0 to ± 100 mV
x20 range: 0 to ± 200 mV
External Signals applied to COMMAND IN÷10 are attenuated by a factor of 10,
1 V applied = 100 mV command
Physical Dimensions Case 8.9 cm x 43.2 cm x 30.5 cm (H x W x D)
Voltage Headstage 12.5 mm D x 5 cm L with 1.8 m cable
Mounting Handle 8.0 mm D x 6.3 cm L
Bath Headstage 2.3 cm x 3.5 cm x 4.2 cm (H x W x D), with 1.8 m cable
Shipping Weight 6.0 kg
Warranty One year, parts & labor
Power Requirements Universal: 100-130 or 220-240 VAC, 50/60 Hz, 14 VA

References

Videos

  1. On-Demand Webinar: Data Acquisition and Recording with TEV-700 Oocyte Workstation
    Warner Instruments
    Vimeo 2013

References

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Pricing Top Description

Current and voltage electrode holders purchased separately

 

Order No. Model No. Product Description USA Price Order
64-3068 OC-725D Oocyte Voltage Clamp Amplifier Login Login
OC-725D Voltage Electrode Holder (Purchased Separately)
64-1009 ESW-F15V E Series Electrode Holder, straight style, fits 1.5 mm capillary, Ag wire Login Login
64-1007 ESW-F10V E Series Electrode Holder, straight style, fits 1.0 mm capillary, Ag wire Login Login
64-1008 ESW-F12V E Series Electrode Holder, straight style, fits 1.2 mm capillary, Ag wire Login Login
64-1010 ESW-F20V E Series Electrode Holder, straight style, fits 2.0 mm capillary, Ag wire Login Login
OC-725D Current Electrode Holder (Purchased Separately)
64-1053 E45W-F15VH E Series Electrode Holder, 45° with handle, fits 1.5 mm capillary, Ag wire Login Login
64-1051 E45W-F10VH E Series Electrode Holder, 45° with handle, fits 1.0 mm capillary, Ag wire Login Login
64-1054 E45W-F20VH E Series Electrode Holder, 45° with handle, fits 2.0 mm capillary, Ag wire Login Login
64-1052 E45W-F12VH E Series Electrode Holder, 45° with handle, fits 1.2 mm capillary, Ag wire Login Login
Accessories and Replacement Parts
64-0029 725-MC Oocyte model cell Login Login
64-3070 Replacement OC-725D bath clamp (must be calibrated to amplifier, not OC-725C compatible) Login Login
64-0033 7259C Replacement OC-725D current electrode cable (OC-725C compatible) Login Login
64-3069 Replacement OC-725D voltage headstage (OC-725C compatible) Login Login
64-3072 Voltage probe handle for OC-725, IE-210, and IE-251 Login Login

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