Specialized Tools for Electrophysiology and Cell Biology Research

(RC-21BDW) Open Diamond Bath Imaging Chamber

An open bath, polycarbonate recording chamber that uses 25 mm round coverslips and incorporates Warner's diamond bath fluidics.

  • Designed for physiological measurements on cell cultured coverslips
  • Identical to the RC-21BRW except for a diamond shaped bath
  • Uses A 25 mm round coverslip as the chamber floor
  • Diamond shaped bath provides laminar solution flow
  • Quiet operation
  • Wicked solution removal from bath
  • Can be used with upright or inverted microscopes

The low profile design of the RC-21BDW allows good electrode access to the chamber bath. Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well. With laminar flow, the sample is minimally distorted during perfusion. Bath volume is 143 µl for each mm solution height.

RC-21BDW chambers require a platform (PM-2PH-2P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.

 

Each RC-21BDW chamber is supplied with a package of #1 thickness glass coverslips (CS-25R, 25 mm dia., pkg. of 100) and 10 ft of PE-160 polyethylene tubing.

Material polycarbonate
Bath dimensions
(L x W x H)
17 x 13 x 3.8 mm
Volume, by depth 143 µl/mm
Bottom coverglass 25 mm round
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Output tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)


 

Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
    Shigetomi E, Khakh BS
    J Vis Exp. 2009 Apr 26;(26). pii: 1142. doi: 10.3791/1142
  2. Tissue-specific Regulation of Sodium/Proton Exchanger Isoform 3 Activity in Na+/H+ Exchanger Regulatory Factor 1 (NHERF1) Null Mice
    Murtazina R, Kovbasnjuk O, Zachos NC, Li X, Chen Y, Hubbard A, Hogema BM, Steplock D, Seidler U, Hoque KM, Tse CM, De Jonge HR, Weinman EJ, Donowitz M
    J Biol Chem. 2007 Aug 24;282(34):25141-51. Epub 2007 Jun 19
Order No. Model No. Product Description USA Price Order
64-1561 PM-2 PM-2 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0705 CS-25R Round cover glass, #1 thickness, 25 mm, 100 pack Login Login
Accessories and Replacement Parts
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Chambers
64-0362 RC-21BDW Open chamber for 25 mm round coverslip, 100 µl volume Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Platforms With Heating Elements
64-0285 PH-2 PH-2 Platform for Series 20 chambers, heater Login Login
Platforms Without Heating Elements
64-0278 P-2 P-2 Platform for Series 20 chambers, non-heater Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

Need support?

Want to know more about our quality products or need assistance in technical support?

TALK TO OUR EXPERTS

NEWSLETTER

For the most up-to-date information on Warner Instruments news and technologies, sign-up for our newsletter.