Specialized Tools for Electrophysiology and Cell Biology Research

(RC-22, RC-22C) Open Diamond Bath Imaging Chambers

Product Summary

The RC-22 and RC-22C are small volume, open bath recording chambers designed for tissue and brain slice studies.

  • Designed for tissue slice samples or cell cultured coverslips
  • Applications include patch clamp, intracellular / extracellular recordings, and imaging
  • Diamond shaped bath provides laminar solution flow
  • Small bath volume facilitates rapid solution exchange
  • Tissue slice anchor included

The RC-22 and RC-22C feature an open bath and include a slice anchor. The diamond shape geometry of these chamber baths facilitate laminar solution flow which can provide minimal distortion of the sample during perfusion.

Slices are secured via a supplied stainless steel anchor (or harp). Threads are made of Lycra© and have a spacing of 1.5 mm. The RC-22 is supplied with an SHD-22L/15 anchor, and the RC-22C is supplied with an SHD-22CL/15 anchor. While anchor mass (typically 300 milligrams) may be sufficient to hold a slice in place, Warner anchors rely on a close fit between the anchor body and the chamber sidewall. This feature can reduce stress on the anchored slice.

 

Solution aspiration is via a stainless steel suction tube which is secured by a clamp. This insures that the adjustable-height suction tube will not move once set by the user. Each chamber has an agar bridge well for reference electrodes. The working volume of the RC-22 and RC-22C is 115 µl and 138 µl, respectively. Chamber sidewalls have a height of 1.2 mm to facilitate electrode access and placement.

The RC-22 and RC-22C chambers require a platform (PM-1PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting onto a microscope. The bottom-forming coverslip is 22 x 40 mm.

 

640277_P1_0

 

Each chamber is supplied with a slice anchor, a swatch of nylon mesh, a pack of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.

  RC-22 RC-22C
Material polycarbonate polycarbonate
Bath dimension
(L x W x H)
22 x 8 x 3.8 mm 23 x 10 x 3.8 mm
Volume, by depth 115 μl/mm 138 μl/mm
Bottom coverglass 22 x 40 mm 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD) 1.67 mm (OD)

 

 

 

 

Order No. Model No. Product Description USA Price Order
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0246 SHD-22L/15 Slice anchor for RC-22, 1.5 mm thread spacing, stainless steel Login Login
64-0247 SHD-22CL/15 Slice anchor for RC-22C, 1.5 mm thread spacing, stainless steel Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0198 NYL/MESH Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

Videos

  1. Perfusion and Aspiration Techniques
    Warner Instruments
    YouTube 2012

References

  1. Tuning the excitability of midbrain dopamine neurons by modulating the Ca2+ sensitivity of SK channels
    Ji H, Hougaard C, Herrik KF, Strøbaek D, Christophersen P, Shepard PD
    Eur J Neurosci. 2009 May;29(9):1883-95. Epub 2009 Apr 20
  2. Excitatory synapses in the rat superficial dorsal horn are strengthened following peripheral inflammation during early postnatal development
    Li J, Baccei ML
    Pain. 2009 May;143(1-2):56-64. Epub 2009 Feb 26
  3. Multiple pathological events in exercised dystrophic mdx mice are targeted by pentoxifylline: outcome of a large array of in vivo and ex vivo tests
    Burdi R, Rolland JF, Fraysse B, Litvinova K, Cozzoli A, Giannuzzi V, Liantonio A, Camerino GM, Sblendorio V, Capogrosso RF, Palmieri B, Andreetta F, Confalonieri P, De Benedictis L, Montagnani M, De Luca A
    J Appl Physiol. 2009 Apr;106(4):1311-24. Epub 2009 Jan 8
  4. Cell Electrophysiology with Carbon Nanopipettes
    Schrlau MG, Dun NJ, Bau HH
    ACS Nano. 2009 Mar 24;3(3):563-8
  5. Peripheral nerve injury sensitizes neonatal dorsal horn neurons to tumor necrosis factor
    Li J, Xie W, Zhang JM, Baccei ML
    Mol Pain. 2009 Mar 2;5:10
  6. The Role of L- and T-Type Calcium Channels in Local and Remote Calcium Responses in Rat Mesenteric Terminal Arterioles
    Braunstein TH, Inoue R, Cribbs L, Oike M, Ito Y, Holstein-Rathlou NH, Jensen LJ
    J Vasc Res. 2009;46(2):138-51. Epub 2008 Sep 2
  7. Decreased number of interneurons and increased seizures in neuropilin 2 deficient mice: Implications for autism and epilepsy
    Gant JC, Thibault O, Blalock EM, Yang J, Bachstetter A, Kotick J, Schauwecker PE, Hauser KF, Smith GM, Mervis R, Li Y, Barnes GN
    Epilepsia. 2009 Apr;50(4):629-45. Epub 2008 Jul 24
  8. Oxidative impairment of hippocampal long-term potentiation involves activation of protein phosphatase 2A and is prevented by ketone bodies
    Maalouf M, Rho JM
    J Neurosci Res. 2008 Nov 15;86(15):3322-30
  9. Gentamicin treatment in exercised mdx mice: Identification of dystrophin-sensitive pathways and evaluation of efficacy in work-loaded dystrophic muscle
    De Luca A, Nico B, Rolland JF, Cozzoli A, Burdi R, Mangieri D, Giannuzzi V, Liantonio A, Cippone V, De Bellis M, Nicchia GP, Camerino GM, Frigeri A, Svelto M, Camerino DC
    Neurobiol Dis. 2008 Nov;32(2):243-53. Epub 2008 Jul 23
  10. Developmental Changes in the Fidelity and Short-Term Plasticity of GABAergic Synapses in the Neonatal Rat Dorsal Horn
    Ingram RA, Fitzgerald M, Baccei ML
    J Neurophysiol. 2008 Jun;99(6):3144-50. Epub 2008 Apr 9
  11. Action potential throughput in aged rat hippocampal neurons: Regulation by selective forms of hyperpolarization
    Gant JC, Thibault O
    Neurobiol Aging. 2008 Mar 24
  12. Excitatory effects of human immunodeficiency virus 1 Tat on cultured rat cerebral cortical neurons
    Brailoiu GC, Brailoiu E, Chang JK, Dun NJ
    Neuroscience. 2008 Feb 6;151(3):701-10. Epub 2007 Dec 5
  13. Odorant receptors directly activate phospholipase C/inositol-1,4,5-trisphosphate coupled to calcium influx in Odora cells
    Guang Liu, Robert M. Badeau, Akihiko Tanimura and Barbara R. Talamo
    Journal of Neurochemistry, 2006, 96, 1591–1605
  14. Perinatal hyperoxia for 14 days increases nerve conduction time and the acute unitary response to hypoxia of rat carotid body chemoreceptors
    David F. Donnelly, Insook Kim, Claire Carle and John L. Carroll
    J Appl Physiol 99: 114-119, 2005

Order No. Model No. Product Description USA Price Order
Chambers
64-0228 RC-22 Recording chamber for tissue slice, with SHD-22L/15 anchor Login Login
64-0229 RC-22C Recording chamber for tissue slice, with SHD-22CL/15 anchor Login Login
Platforms
64-1526 PM-1 PM-1 Platform for Series 20 chambers, heated, magnetic clamps Login Login
64-0284 PH-1 PH-1 Platform for Series 20 chambers, heater Login Login
64-0277 P-1 P-1 Platform for Series 20 chambers, non-heater Login Login
Slice anchors (Harps) - RC-22
64-0263 SHD-22KIT Slice anchor kit for RC-22, stainless steel Login Login
64-0248 SHD-22L/10 Slice anchor for RC-22, 1.0 mm thread spacing, stainless steel Login Login
64-0246 SHD-22L/15 Slice anchor for RC-22, 1.5 mm thread spacing, stainless steel Login Login
64-0198 NYL/MESH Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk Login Login
64-1412 SHD-22F/10 Slice anchor for RC-22, 1.0 mm thread spacing, flat frame, stainless steel Login Login
64-1411 SHD-22F/15 Slice anchor for RC-22, 1.5 mm thread spacing, flat frame, stainless steel Login Login
Slice anchors (Harps) - RC-22C
64-0264 SHD-22CKIT Slice anchor kit for RC-22C, stainless steel Login Login
64-0249 SHD-22CL/10 Slice anchor for RC-22C, 1.0 mm thread spacing, stainless steel Login Login
64-0247 SHD-22CL/15 Slice anchor for RC-22C, 1.5 mm thread spacing, stainless steel Login Login
64-0198 NYL/MESH Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk Login Login
64-1414 SHD-22CF/10 Slice anchor for RC-22C, 1.0 mm thread spacing, flat frame, stainless steel Login Login
64-1413 SHD-22CF/15 Slice anchor for RC-22C, 1.5 mm thread spacing, flat frame, stainless steel Login Login
Replacement Consumables
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
64-1920 SA-20PS1 Adapter Ring for Pecon Heating Insert P Login Login
Replacement Parts
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login

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