The lowest noise, switchable, resistive-feedback patch clamp amplifier currently available.
The PC-505B is the lowest noise, switchable, resistive-feedback patch clamp amplifier currently available. This new model also has features of particular interest to those doing whole cell studies. The slow capacitance compensation circuitry has been combined into a single control and allows direct measurement of membrane capacitance. The companion Series R control displays the access resistance and the new % correction circuit compensates up to 90% of the access resistance. These and other new features make the PC-505B an extremely capable amplifier.
Two selectable feedback resistors in the headstages permit both single channel and whole cell recording in the same cell. A 50 GΩ resistor is used in both switching models for low noise single channel recording with currents to 200 pA.
The PC-505B has three modes of operation: voltage clamp, zero current clamp, and current clamp.
Headstage feedback resistance is dynamically switched at the amplifier front panel. LEDs indicate resistor selection and corresponding multiplying factor applied to the current gain [Im] switch setting.
Voltage and current commands applied to the cell include: voltage and current hold, junction and auto zero, test pulse, speed test, and zap.
Stray capacitance between the input and electrode resistance is compensated with two pair of controls, C-Fast 1 and C-Fast 2. Amplitude and time constant of each pair is independently adjustable. Whole cell capacitance compensation is adjusted with the single control, C-Slow. Membrane capacitance is read from the C-Slow calibrated dial. The companion Series R control is used in conjunction with the C-Slow and its calibrated dial provides a reading of the access resistance. C-Slow may be disabled to view the uncompensated signal. The % Correction control is used to increase the command signal to compensate for the voltage drop across the access (Series R) resistance. Correction is adjustable up to 90%.
Signals at the Im output are filtered at the selected cutoff frequency set with the 4-pole Bessel filter. Selecting Bypass presents the full bandwidth signal to the Im output. Filtered and unfiltered Im output is also available at the instrument rear panel. Additional outputs are the membrane voltage Vm x10 and the sum of the voltage commands Vc x10, both at x10 gain.
Warner patch clamp holders are made from polycarbonate to minimize recording noise. Holders are custom-bored for glass diameters from 1 to 2 mm to minimize lateral movement of the pipette. Electrical coupling to the pipette is made with a silver wire. Holders are not included with the PC-505B. They must be purchased separately based on your glass outer diameter.
The PC-505B functions as an excellent low-noise potentiostat for voltammetric and other electrochemical measurements. In this mode, the V hold (electrode potential) is increased to a maximum of ±1 volt and the maximum external command signal to ±2 volts at electrode or sensor.
|LC-201B Headstage||Single channel currents to 200 pA, whole cell currents to 20 nA. (50 GΩ/500 MΩ)|
|HC-202B Headstage||Single channel currents to 200 pA, whole cell currents to 200 nA.(50 GΩ/50 MΩ)|
|WI-205B Bilayer Headstage||For artificial bilayer capacitances up to 250 pF, currents to 200 pA. (50 GΩ, modified)|
|Noise (referred to input)||Measured with an 8-pole Bessel filter, input open, 50 GΩ resistor:|
|DC to 1 kHz||0.035 pA RMS|
|DC to 5 kHz||0.150 pA RMS|
|Voltage Clamp Commands|
|Command In BNC||± 10 V Max, AC or DC, applied to input|
|Voltage Hold||± 200 mV Max with 10-turn control|
|Junction Zero||± 100 mV Max with 10-turn control|
|Internal Test Pulse||1 V 50/60 Hz (line freq.) square wave attenuated by Command Sensitivity|
|Zap||1.0 V Pulse, adjustable duration from 0.1 to 10 msec|
|Current Clamp Commands|
|Current Hold||± 1 nA with 10-turn control|
|Internal Test Pulse||1 nA 100 Hz square wave through Command Sensitivity|
|Command In BNC||± 10 V max, AC or DC, applied to input|
|Fast Capacitance Compensation (Voltage Mode|
|C Fast 1||0.1 to 1.75 µsec, 0 to 5 pF|
|C Fast 2||0.33 to 8.5 µsec, 0 to 15 pF|
|Whole Cell Compensation|
|C Slow||0-100 pF with 10 turn control|
|Series R||0-100 MΩ with 10 turn control|
|% Correction||0-90% of Series R|
|to 50 GΩ||50 GΩ Headstage Resistor|
|to 500 MΩ||500 MΩ Headstage Resistor|
|to 50 MΩ||50 MΩ Headstage Resistor|
|Front Panel Outputs|
|Im (membrane current)||
|Vc x10||Summation of all commands amplified by 10|
|Vm x10||Membrane voltage amplified by 10|
|Im Low-Pass Filter||0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 kHz and Bypass (4-Pole Bessel)|
|Panel Meter||3-1/2 Digit LED reads (full scale):
|RMS Noise||1.999 pA|
|Rear Panel Outputs|
|Gain Telegraph||From 0.5 to 7.0 V in 0.5 V steps*|
|Filter Telegraphs||From 0.2 to 2.0 V in 0.2 V steps*|
|Im/Vm Telegraph||Logic levels, V-Clamp=1, I-Clamp=0|
|Sync Out||Signal for synchronizing an oscilloscope to internal test|
|Power||110 to 130 or 220 to 250 VAC, 50/60 Hz, 15 VA|
|Main Unit||8.9 cm H x 43.2 cm W x 30.5 cm D|
|Headstage||1.9 cm H x 3.5 cm W x 5.7 cm L with 1.8 m cable|
|Mounting Rod||6.3 mm D x 6.3 cm L|
|Shipping Weight||11.4 kg|
|Warranty||Three years, parts and labor|
|Order No.||Model No.||Product Description||USA Price||Order|
|64-0000||PC-505BLC||PC-505B patch clamp with LC-201B headstage (50 GΩ/500 MΩ)||Login||Login|
|64-0001||PC-505BHC||PC-505B patch clamp with HC-202B headstage (50 GΩ/50 MΩ)||Login||Login|
|64-0002||PC-505BHB||PC-505B patch clamp with HB-205B bilayer headstage (50 GΩ/500 MΩ)||Login||Login|
|Electrode Holders (ordered separately)|
|64-0821||QSW-A10P||Q Series electrode holder, port, straight style, fits 1.0 mm capillary, Ag wire||Login||Login|
|64-0822||QSW-A12P||Q Series electrode holder, port, straight style, fits 1.2 mm capillary, Ag wire||Login||Login|
|64-0823||QSW-A15P||Q Series electrode holder, port, straight style, fits 1.5 mm capillary, Ag wire||Login||Login|
|64-0978||QSW-A17P||Q Series electrode holder, port, straight style, fits 1.7 mm capillary, Ag wire||Login||Login|
|64-0824||QSW-A20P||Q Series electrode holder, port, straight style, fits 2.0 mm capillary, Ag wire||Login||Login|
|64-0455||PV-830||Power Line Conditioner 1000 VA, 120 VAC 60HZ||Login||Login|
|64-0456||PV-830CE||Power Line Conditioner 1000 VA, 220 VAC 50/60HZ||Login||Login|
|64-1505||HE-6||Handle extension, 7.9 mm dia x 6.3 cm long, 6-32 male threaded end||Login||Login|
|64-0004||LC-201B||50 GΩ/500 MΩ switchable||Login||Login|
|64-0005||HC-202B||50 GΩ/50 MΩ, switchable||Login||Login|
|64-0006||HB-205B||50 GΩ (Bilayer)/500 MΩ, switchable||Login||Login|
|89-5035||LIH 8+8||LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE||Login||Login|