Specialized Tools for Electrophysiology and Cell Biology Research

pco.edge 4.2 USB

Product Summary

The pco.edge 4.2 USB is equipped with an innovative scientific CMOS sensor that provides crisp images and precise measurements

  • 0.8 e-med readout noise
  • 2048 x 2048 pixel resolution
  • up to 37500:1 dynamic range
  • 100 fps maximum frame rate
  • up to 82% quantum efficiency
  • CameraLink or USB 3.0 interface
  • optional water cooling system

 

Groundbreaking sCMOS technology for outstanding image quality

pco.edge 4.2 USB
Patch Clamp Amplifier (PC-505B)
pco.edge 4.2 USB

superior image quality

The pco.edge sCMOS camera features outstanding low read out noise.

Even at maximum speed of 40 frames/s at full resolution of 2048 x 2048 pixel the noise is 0.8 e- (med). Moreover the pco.edge provides an excellent homogeneous pixel response to light (PRNU, photo response non-uniformity) and an excellent homogeneous dark signal pixel behaviour (DSNU,dark signal non-uniformity) which is achieved by a sophisticated electronic circuit technology and firmware algorithms.

high resolution

The pco.edge sCMOS camera features outstanding low read out noise.

A 4.2 Mpixel resolution in combination with a moderate chip size (18.8 mm diagonal, 6.5 µm pixel pitch) benefts microscopy applications with low magnifcation factor and large feld of view, thereby reducing processing times and increasing throughput. The figure compares the potential of the new feld of view of the pco.edge to the 1.3 Mpixel image resolution which is widely used in microscopy applications for scientifc cameras

pco.edge 4.2 USB

rolling shutter readout modes

pco.edge sCMOS cameras feature a variety of precise synchronization modes which are optimized for advanced microscopy imaging and scanning.

The flexible frame and line triggers with very low latency and, in combination with the free selectable readout modes, can easily be combined to cover every modern microscopy situation

For example, one mode is used in a lightsheet or SPIM application, the lower right rolling shutter operational mode “single top down” operation is convenient to properly synchronize the camera exposure with the scanner. On the other hand, if speed is required and a ?ash like exposure is applied the upper left mode “dual outside in” is used for localization microscopy techniques like GSD, PALM or STORM.

pco.edge 4.2 LT CMOS Camera

free of drift

The pco.edge sCMOS cameras feature temperature stabilized Peltier cooling. This allows for continuous drift free operation in image sequences capture. This is achieved by the proper selection and sophisticated combination of electronics and FPGA algorithms.

As the measurement result shows while running at full speed of 100 frames/s over 4 hours measuring time the camera doesn’t show any signifcant drift. This degree of stability enables long-term measuring series, which should be quantitatively evaluated and processed. For example, in PCR (Polymerase Chain Reaction) applications, when so-called melting curves must be measured, the fuorescence in multi-well plates with different samples is recorded over a longer time at different sample temperatures. Here all the images are used for processing, which is only possible if the offset is stable and the camera is free of drift.

pco.edge 4.2 USB

flexibility and free of latency

User selectable choice of rolling shutter modes for exposure provides fexibility for a wide range of applications.

The advantages of rolling shutter are high frame rates and low read out noise. Due to realtime transmission of the image data to the PC, there is no latency between recording and access or storage of the data.

37500:1 dynamic range

Due to the excellent low noise and the high fullwell capacity of the sCMOS image sensor an intra scene dynamic range of better than 37500:1 is achieved.

A unique architecture of dual column level amplifers and dual 11 bit ADCs is designed to maximize dynamic range and to minimize read out noise simultaneously. Both ADC values are analyzed and merged into one high dynamic 16 bit value.

pco.edge 4.2 LT CMOS Camera
pco.edge 4.2 USB
image sensor
type of sensor scientific CMOS (sCMOS)
image sensor CIS2020A
resolution (H x V) 2048 x 2048 active pixel
pixel size (H x V) 6.5 x 6.5 µm
sensor format /diagonal 13.3 x 13.3 mm /18.8 mm
shutter modes rolling shutter (RS), with free selectable readout modes
MTF 76.9 lp/mm (theoretical)
fullwell capacity (typ) 30000 e-
readout noise 0.8 med / 1.3 rms e- @ slow scan
0.9 med / 1.4 rms e- @ fast scan
dynamic range 37500:1 (91.5 dB) slow scan
quantum efficiency 82% @ peak
spectral range 370-1100 nm
dark current (typ) < 0.6 e- /pixel /s @ 7 °C
DSNU < 0.3 rms e-
PRNU < 0.3%
anti blooming factor > 10000
camera
frame rate 100 fps @ RS, fast scan @ 2024 x 2024 pixel
exposure / shutter time 100 µs - 10 s (RS)
A/D dynamic range 16 bit
A/D conversion factor 0.46 e- /count
pixel scan rate 274 MHz fast scan
100 MHz slow scan
pixel data rate 548 Mpixel/s fast scan
200 Mpixel/s slow scan
binning horizontal x1, x2, x4
binning vertical x1, x2, x4
region of interest (ROI) horizontal: 16 pixel steps
vertical: 1 pixel steps
non-linearity < 0.5%
cooling method 7 °C stabilized
peltier with forced air (fan)
(up to 27 °C ambient)
trigger input signals frame trigger, sequesnce trigger, programmable input (SMA connections)
trigger output signals exposure, busy, line, programmable output (SMA connectors)
data interface Camera Link HS (Single-F2, 1X1, S10)
time stamp in image (1 us resolution)
general
power supply 12-24 VDC (± 10%)
power consumption 32 W (typ 19 W @ 20 °C)
weight 1010 g with F-mount
operating temperature 10-40 °C
operating humidity range 10-80% (non-condensing)
storage temperature range -10 to 60 °C
optical interface C-mount
F-mount
CE / FCC certified yes

 

 

frame rate table
dimension fast scan slow scan
2048 x 2048 100 fps 35 fps
2048 x 1024 200 fps 70 fps
2048 x 512 400 fps 140 fps
2048 x 256 800 fps 281 fps
2048 x 128 1600 fps 562 fps
1920 x 1080 189 fps 66 fps
1600 x 1200 170 fps 60 fps
1280 x 1024 200 fps 70 fps
640 x 480 420 fps 150 fps
320 x 240 853 fps 300 fps

 

 

 

 

OKOLab StageTop Incubator System Overview

Click to enlarge

 

 

Order No. Model No. Product Description USA Price Order
69-3131 edge 4.2 USB pco.edge 4.2 USB, CMΩS camera Login Login

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