Specialized Tools for Electrophysiology and Cell Biology Research

(RC-37W, RC-37F, RC-37WC, RC-37FC, RC-37WS, RC-37FS) Perfusion Inserts for 35 mm Dishes

RC-37 Series inserts enable on-microscope assays using the popular glass bottomed 35 mm cell culture dishes.

  • Designed for glass bottomed 35 mm cell culture dishes
  • Permits imaging, recording, and perfusion in the culture dish
  • Open and closed bath designs
  • Designs for electric field stimulation available
  • Incorporates Warner's diamond bath fluidics
  • Ideal for rapid screening assays

The RC-37 Series Cell Culture Dish Inserts, which include the RC-37WRC-37FRC-37WCRC-37FCRC-37WS, and RC-37FS models, are designed to create a Warner style perfusion chamber within a 35 mm culture dish environment.

The introduction of a thin, glass bottom on 35 mm culture dishes expanded the use of these popular tools dramatically. Applications such as live cell imaging, confocal microscopy, and time lapsed photography became possible. The ability, however, to easily exchange the extracellular environment by perfusion remained difficult at best, until now.

 

Warner's RC-37 Series inserts make perfusion of cell culture dishes a simple matter. A feature in common with these inserts is the use of the glass bottomed, 35 mm culture dish for the floor of the chamber. In most cases, this same culture dish contains the imaging sample. When viewed with inverted microscopes, images are visualized through a single thickness of glass, usually 0.13-0.17 mm. These inserts incorporate Warner's diamond-shaped bath which has been shown to produce a laminar flow across the chamber. Since bath volumes are generally small, exchange times are measured in seconds even when flow rates are less than 1 ml/min.

Perfusion inserts are designed for use with 35 mm cell culture dishes from Willco Wells (D35522P and D3522B), Corning (25000), BD/Falcon (35-4077), MatTek, and NUNC. Note that the W version of RC-37 Series inserts specifically fit the Willco Wells dishes and the F version fits all the others listed above.

Perfusion through RC-37 Series inserts can be managed by a VC-8 or VC-6 perfusion system

 

Open Perfusion Inserts for Cell Culture Dishes

The RC-37W and RC-37F inserts are small volume imaging chambers featuring rapid solution exchange, short working distances and an open bath. These inserts were designed to be securely inserted into a 35 mm culture dish allowing a variety of assays to be performed on cultured cells. The RC-37W is designed for use with the Willco Wells dishes and the RC-37F is designed for use with Corning, Falcon, NUNC, and MatTek dishes.

Fluid is delivered to a perfusion inlet port and removed from the chamber by means of a height adjustable aspirator. These open perfusion dish inserts allow for the easy insertion of electrodes and enable the simultaneous perfusion, imaging, and recording of cells attached to the glass bottom. Included with these inserts is both C-Flex and PE-160 tubing.

Closed Perfusion Inserts for Cell Culture Dishes

The closed bath design of the RC-37WC and RC-37FC promotes an even solution exchange and eliminates changes in focus due to alterations in bath height. In addition, studies involving physiological bicarbonate buffers can be performed with no gas loss at the chamber interface. Fluid is delivered to an 18 gauge perfusion inlet port and removed from the chamber by means of an outlet port of the same gauge.

The top coverslip is held in place with a retainer and the dish becomes the chamber bottom. The RC-37WC uses a 15 mm top coverslip and the RC-37FC uses an 18 mm top coverslip. The distance between top and bottom coverslip on both chambers is 1 mm. The RC-37WC fits dishes from Willco Wells while the RC-37FC fits dishes from Corning, Falcon, NUNC, and MatTek.

The RC-37WC is supplied with one box of CS15R coverslips (#1 thickness, 15 mm round, 100/pkg) and the RC-37FC is supplied with one box of CS18R coverslips (#1 thickness, 18 mm round, 100/pkg). Both chambers are supplied with 10 feet of PE-160 polyethylene tubing, 1 foot of C-Flex tubing, and tools to remove the top coverslip retainer.

 

Perfusion Inserts with Electric Field Stimulation for Cell Culture Dishes

The RC-37WS and RC-37FS perfusion chamber inserts are designed for applications requiring field stimulation and incorporate a pair of platinum electrodes into the periphery of the bath area. Both inserts feature a slotted bath to promote a laminar solution flow and to insure a uniform electric field. These models include a perfusion inlet port and a 16 gauge, height adjustable aspirator. An agar bridge well is provided to accommodate a bath ground electrode.

 

To facilitate field stimulation studies, Warner Instruments offers a virety of field stimulators for applying constant current and/or voltage, or for the application of bipolar stimulation pulses.

The RC-37WS fits Willco Wells dishes while the RC-37FS fits Corning, MatTek, NUNC, and Falcon dishes. Both chambers are supplied with 10 feet of PE-160 polyethylene tubing.

Model Dimensions
ID x OD, mm
Slot w x l, mm
Volume
RC-37W 9.4 x 27.0 mm 110 µl/mm
RC-37F 15.2 x 34.5 mm 220 µl/mm
RC-37WC 9.4 x 27.7 mm 113 µl/mm
RC-37FC 15.2 x 34.0 mm 185 µl/mm
RC-37WS 6.0 x 18.0 mm 105 µl/mm
RC-37FS 6.0 x 24.0 mm 144 µl/mm

 

Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0166 CFL-6 C-Flex tubing, 1/32 x 6 (ID x ft), for pinch valves Login Login
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-0100 TC-324B TC-324B Heater Controller, single channel Login Login
64-0101 TC-344B TC-344B Heater Controller, dual channel Login Login
Order No. Model No. Product Description USA Price Order
64-0363 RC-37WC Closed bath chamber insert for Willco 35 mm cell culture dishes Login Login
64-0364 RC-37FC Chamber insert for Falcon 35 mm cell culture dishes, closed bath Login Login
64-0365 RC-37WS Chamber Insert for Willco 35 mm cell culture dishes, with field stimulation Login Login
64-0366 RC-37FS Chamber Insert for Falcon 35 mm cell culture dishes, with field stimulation Login Login
64-0375 QE-1 Quick change platform, heated base, for RC-40 and QR-40 series chambers Login Login
64-0110 DH-35 35 mm culture dish heater Login Login
64-1424 SIU-102 Bipolar stimulator, high current, non-isolated Login Login
64-0349 DH-35iL Culture dish incubation system Login Login
Accessories
64-0348 RC-37F Chamber Insert for Corning, MatTek, Nunc, and Falcon 35 mm cell culture dishes Login Login
64-0347 RC-37W Chamber Insert for Willco 35 mm cell culture dishes Login Login
Accessories and Replacement Parts
64-0102 SH-27B In-line solution heater Login Login
64-0103 SF-28 Slow flow in-line solution heater Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1425 CC-102 Cable, BNC + male banana to 1 mm jack (for use with field stimulation chambers) Login Login
64-0166 CFL-6 C-Flex tubing, 1/32 x 6 (ID x ft), for pinch valves Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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