(RC-37W, RC-37F, RC-37WC, RC-37FC, RC-37WS, RC-37FS) Perfusion Inserts for 35 mm Dishes

The RC-37 Series Cell Culture Dish Inserts, which include the RC-37W, RC-37F, RC-37WC, RC-37FC, RC-37WS, and RC-37FS models, are designed to create a Warner style perfusion chamber within a 35 mm culture dish environment.

The introduction of a thin, glass bottom on 35 mm culture dishes expanded the use of these popular tools dramatically. Applications such as live cell imaging, confocal microscopy, and time lapsed photography became possible. The ability, however, to easily exchange the extracellular environment by perfusion remained difficult at best, until now.

The RC-37W and RC-37F inserts are small volume imaging chambers featuring rapid solution exchange, short working distances and an open bath. These inserts were designed to be securely inserted into a 35 mm culture dish allowing a variety of assays to be performed on cultured cells. The RC-37W is designed for use with the Willco Wells dishes and the RC-37F is designed for use with Corning, Falcon, NUNC, and MatTek dishes.

The closed bath design of the RC-37WC and RC-37FC promotes an even solution exchange and eliminates changes in focus due to alterations in bath height. In addition, studies involving physiological bicarbonate buffers can be performed with no gas loss at the chamber interface. Fluid is delivered to an 18 gauge perfusion inlet port and removed from the chamber by means of an outlet port of the same gauge.

The RC-37WS and RC-37FS perfusion chamber inserts are designed for applications requiring field stimulation and incorporate a pair of platinum electrodes into the periphery of the bath area. Both inserts feature a slotted bath to promote a laminar solution flow and to insure a uniform electric field. These models include a perfusion inlet port and a 16 gauge, height adjustable aspirator. An agar bridge well is provided to accommodate a bath ground electrode.