Specialized Tools for Electrophysiology and Cell Biology Research

(PLI-90A) Picoliter Injector

Product Summary

The PLI-90A picoinjector reliably delivers injections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time.

  • Femtoliter to microliter injections
  • Digital readouts for injection count, time, and pressure
  • Reliable optically encoded circuit for setting injection time
  • 3 pressures: inject, balance, and clear

Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-90A is up to the task.

Pressure to the pipette is controlled precisely through a multi-turn regulator and is reported digitally for reproducibility. Injection time is set using an optically encoded circuit that permits both fine and coarse settings from a single control knob. System timing can be controlled in three ways: push button, foot switch, or external trigger.

Unique Pressure Capabilities

The PLI-90A features three positive pressures.

These functions allow the user to:

  • Inject - Inject picoliter to nanoliter amounts of fluids
  • Balance - Apply a balance pressure to prevent backflow into the micropipette following an injection
  • Clear - Clear a micropipette of material that is blocking the tip

The PLI-90A's Balance and Clear functions are features not found on other microinjection units.

Balance
In addition to the injection pressure, the PLI-90A offers a secondary balance pressure. This adjustable control applies a positive pressure to the injection pipette both before and after an injection that eliminates dilution caused by capillary action and aids in the prevention of clogging. Wasteful continuous injections that can occur when no separate balance pressure is available is avoided.
Clear
A high-pressure pulse can be used for clearing a pipette should it happen to clog. This is especially useful when working with pipettes sized for smaller volumes.

Electrical Connectors

BNC type connectors are available at the front panel to ease integrating the PLI with other equipment. Synchronization with or control from injections with a data acquisition system is possible.

Selection Guideline

The PLI-90A is available in two packages: Basic and Plus:

The Basic system comes with an input hose, output hose, and input hose adapter.
The Plus system comes with everything in the Basic system with the addition of one foot switch and a stainless steel pipette holder.

All PLI-90A's require a micromanipulator and support stand, which must be purchased separately.
For labs lacking bottled gas or pressurized air, the AIR-1 compressor is available.

Input Gas Pressure 75 PSI Recommended, 105 PSI Maximum
Injection Pressure 0.2 to 60 PSI (413 kPa), regulated
Balance Pressure 0.1 to 9.9 PSI (68.9 kPa) regulated, multiturn control, other ranges available on request
Clearing Pressure Input gas pressure, unregulated
Injection Time 0.01 to 99.99 Seconds
Injection Time Accuracy ±0.01% (Crystal Time Base)
Duration Mode Internally timed or externally gated
Time Trigger Front panel, foot switch, or external TTL pulse (BNC)
Pressure Units PSI /kPa switch selectable
Pressure Monitor Output BNC, front panel, 10 mV/PSI or 1 mV/kPa
Pressure Readout Inject, balance, clear, output port
Pressure Display Resolution 3½ Digits, 0.1 PSI or 1 kPa
Injection Count Display 0 to 9999 Injections
Power Input External 9VDC @ 400mA (min) Power Supply universal input 90 to 264 VAC
Weight 5.44 kg (12 lb)
Dimensions (HxWxD) 89 x 432 x 250 mm (H x W x D)
Warranty One year, parts & labor
641738_PLI90A_back

Rear Panel

Picoliter Injector (PLI-90A)
Pipette Holder

Output Hose

Order No. Model No. Product Description USA Price Order
64-1738 PLI-90A PLI-90A Basic Pico Injector with Injection, Balance, and Clear Pressures; Comes with the following: Input Hose, Output Hose, Input Hose Adapter, and universal power supply Login Login
64-1739 PLI-90A Plus PLI-90A Plus Pico Injector with Injection, Balance, Clear, and Pressures; Comes with the following: input Hose, Output Hose, Foot Switch (65-0029), Pipette Holder (65-0013), Input Hose Adapter, and universal power supply Login Login
65-0013 PLI-PH1 Stainless Steel Pipette Holder, 130 mm L for 1.0 - 1.5 mm OD Glass Pipettes Login Login
65-0014 PLI-PH1A Stainless Steel Pipette Holder, 80 mm L for 1.0 - 1.5 mm OD Glass Pipettes Login Login
65-0017 PLI-SRG Silicone rubber gasket replacement for use with 1.0 mm glass capillaries (fits PLI-PH-1 & PLI-PH-1A holders), 10.2 cm Login Login
65-0015 PLI-SRG1.5 Silicone rubber gasket replacement for use with 1.5 mm glass capillaries (fits PLI-PH-1 & PLI-PH-1A holders), 10.2 cm Login Login
65-0018 PLI-SSB Stainless Steel Bushing Replacement for use with PLI-PH-1 and PLI-PH-1A Pipette Holders Login Login
65-0019 PLI-PC Stainless Steel Pipette Cap Replacement for use with PLI-PH-1 and PLI-PH-1A Pipette Holders Login Login
65-0020 PLI-HN Stainless Steel Hose Nut Replacement for use with PLI-PH-1 and PLI-PH-1A Pipette Holders Login Login
65-0021 PLI-PH-KIT Pipette holder hardware replacement kit for use with PLI-PH-1 and PLI-PH-1A pipette holders Login Login
65-0022 PLI-PHA Stainless Steel Pipette Holder Adapter (PLI-PH series) for Eppendorf ECET FEMTO-TIP Login Login
64-1626 AO161.0 Acrylic pipette holder for 1.0 mm pipettes Login Login
64-1627 AO161.2 Acrylic pipette holder for 1.2 mm pipettes Login Login
64-1628 PLI-PPH Acrylic Pipette Holder for 1.5 mm pipettes Login Login
64-1629 AO162.0 Acrylic pipette holder for 2.0 mm pipettes Login Login
64-0055 MM-33L Micromanipulator with clamp base, left handed Login Login
64-0056 MM-33R Micromanipulator with clamp base, right handed Login Login
64-0057 MM-33LT Micromanipulator with tilt base, left handed Login Login
64-0058 MM-33RT Micromanipulator with tilt base, right handed Login Login
69-0225 MB-B Magnetic base, main pole (12 mm D 176 mm L), sub pole (10 mm D 165 mm L), 800 N hold force Login Login
65-0029 PLI-FS Foot switch (compatible with PLI-100A, PLI-90A and PLI-10) for inject, hold, fill, gate Login Login
64-1701 AIR-1 Ultra low noise air compressor, 120 VAC/60Hz Login Login
65-0006 PLI-IHA Input hose adapter, 1/4 in male NPT pipe thread gas bottle regulator fitting Login Login
65-0008 PLI-IHN Gas input hose (Tygon tubing), 0.250 x 0.125 in (OD x ID), quick connect/disconnect, 9 ft Login Login
65-0010 PLI-OHN Output hose (Tygon tubing) 9 ft L x 0.071 in x 0.039 in (OD x ID), quick connect/disconnect type Login Login
65-0036 PLI-100 Foot switch (legacy design, compatible with PLI-100 and PLI-90, 3.5 mm mono jack) for inject, hold, fill, gate Login Login
65-0007 PLI-IHO Gas input hose (compatible with PLI-100 and PI-90,Tygon tubing), 0.250 x 0.125 in (OD x ID), brass screw fitting, 9 ft Login Login
65-0009 PLI-OHO Output hose (compatible with PLI-100 and PI-90,Tygon tubing) 9 ft L x 0.071 in x 0.039 in (OD x ID), brass screw fitting type Login Login

Need support?

Want to know more about our quality products or need assistance in technical support?

TALK TO OUR EXPERTS

NEWSLETTER

For the most up-to-date information on Warner Instruments news and technologies, sign-up for our newsletter.