Specialized Tools for Electrophysiology and Cell Biology Research

(PLI-100A) Picoliter Microinjector

The PLI-100A picoinjector reliably delivers injections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time.

  • Femtoliter to microliter injections
  • Digital readouts for injection count, time, and pressure
  • Reliable optically encoded circuit for setting injection time
  • 5 pressures: inject, balance, clear, fill, and hold

Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-100A is up to the task.

Pressure to the pipette is controlled precisely through a multi-turn regulator and is reported digitally for reproducibility. Injection time is set using an optically encoded circuit that permits both fine and coarse settings from a single control knob. System timing can be controlled in three ways: push button, foot switch, or external trigger.

Unique Pressure Capabilities

The PLI-100A features three positive and two negative pressures.

The three positive functions allow the user to:
  • Inject - Inject picoliter to nanoliter amounts of fluids
  • Balance - Apply a balance pressure to prevent backflow into the micropipette following an injection
  • Clear - Clear a micropipette of material that is blocking the tip
The two negative (or vacuum) functions allow the user to:
  • Fill - Fill micropipettes from their tips
  • Hold - Provide a means to secure and manipulate a cell using holding pipette.

The PLI-100A's Balance, Hold, and Clear functions are features not found on other microinjection units.

Balance
In addition to the injection pressure, the PLI-100A offers a secondary balance pressure. This adjustable control applies a positive pressure to the injection pipette both before and after an injection that eliminates dilution caused by capillary action and aids in the prevention of clogging. Wasteful continuous injections that can occur when no separate balance pressure is available is avoided.
Fill
The PLI-100A has two separate vacuum generators used to fill a micropipette and to hold suspended cells while injecting. Filling of the pipette from the tip is less complicated than back-filling. The PLI-100A features a Fill Vacuum adjustment on the front panel.
Hold
Suspended cells can be held in place using a second (holding) pipette. The holding vacuum range accommodates most cell types.
Clear
A high-pressure pulse can be used for clearing a pipette should it happen to clog. This is especially useful when working with pipettes sized for smaller volumes.

Electrical Connectors

BNC type connectors are available at the front panel to ease integrating the PLI with other equipment. Synchronization with or control from injections with a data acquisition system is possible.

Selection Guideline

The PLI-100A is available in three packages: Basic, Plus, and Deluxe:

The Basic system comes with an input hose, output hose, holding hose and input hose adapter.
The Plus system comes with everything in the Basic system with the addition of one foot switch and a stainless steel pipette holder.
The Deluxe system comes with everything in the Plus system with the addition of a second foot switch and a second stainless steel pipette holder with input hose.

All PLI-100A's require a micromanipulator and support stand, which must be purchased separately.
For labs lacking bottled gas or pressurized air, the AIR-1 compressor is available.

Input Gas Pressure 75 PSI Recommended, 105 PSI Maximum
Injection Pressure 0.2 to 60 PSI (413 kPa), regulated
Balance Pressure 0.1 to 9.9 PSI (68.9 kPa) regulated, multiturn control, other ranges available on request
Fill Vacuum Internally produced 0 to -12 PSI (0 to-82 kPa), regulated
Holding Vacuum Internally produced 0 to -0.1 PSI (0 to -0.75 kPa), regulated
Clearing Pressure Input gas pressure, unregulated
Injection Time 0.01 to 99.99 Seconds
Injection Time Accuracy ±0.01% (Crystal Time Base)
Duration Mode Internally timed or externally gated
Time Trigger Front panel, foot switch, or external TTL pulse (BNC)
Pressure Units PSI /kPa switch selectable
Pressure Monitor Output BNC, Rear Panel, 10 mV/PSI or 1 mV/kPa
Pressure Readout Inject, balance, clear, output port
Pressure Display Resolution 3½ Digits, 0.1 PSI or 1 kPa
Injection Count Display 0 to 9999 Injections
Power Input External 9VDC @ 400mA (min) Power Supply universal input 90 to 264 VAC
Weight 5.44 kg (12 lb)
Dimensions (HxWxD) 89 x 432 x 250 mm (H x W x D)
Warranty One year, parts & labor

 

Rear Panel

Rear Panel

Picoliter Microinjector (PLI-100A)
Picoliter Microinjector (PLI-100A)
Holding Hose with Cell Release Bulb
Output Hose

Picoliter Microinjector (PLI-100A)

Pipette Holder (65-0013)

Order No. Model No. Product Description USA Price Order
65-0029 PLI-FS Foot switch (compatible with PLI-100A, PLI-90A and PLI-10) for inject, hold, fill, gate Login Login
65-0030 PLIRM Frame to mount PLI-90 or PLI-100 in 19 in wide instrument rack Login Login

References

References

  1. Shared versus Specialized Glycinergic Spinal Interneurons in Axial Motor Circuits of Larval Zebrafish
    Liao JC, Fetcho JR
    J Neurosci. 2008 Nov 26;28(48):12982-92
  2. Pipette Internal Perfusion: Methods and Applications
    Srinivas M. Tipparaju and Aruni Bhatnagar
    Neuromethods, Volume 38, 2007, 309-324
  3. Information Fusing Control of the DNA Microinjection Volume Based on Stochastic Fuzzy Neural Network,
    Zhang Ling, Yu Yongquan, Zeng Bi
    2006-02-21 09:22:58.0
  4. Fluid Dynamics in Conically Tapered Microneedles,
    Wijaya Martanto, Stephen M. Baisch, Elizabeth A. Costner, Mark R. Prausnitz, Marc K. Smith
    AIChE Journal, Vol.51, No.6, 1599-1607, 2005
  5. Endothelial and smooth muscle cell conduction in arterioles controlling blood flow
    Welsh DG, Segal SS
    Am J Physiol. 1998 Jan;274(1 Pt 2):H178-86
  6. Measurement of volume injected into individual cells by quantitative fluorescence microscopy,
    Lee GM.,
    J Cell Sci. 1989 Nov;94 ( Pt 3):443-7
  7. Easy-to-use equipment for the accurate microinjection of nanoliter volumes into the nuclei of amphibian oocytes,
    Stephens DL, Miller TJ, Silver L, Zipser D, Mertz JE.
    Anal Biochem. 1981 Jul 1;114(2):299-309.
  8. Controlled micro release of pharmacological agents: measurements of volume ejected in vitro through fine tipped glass microelectrodes by pressure,
    M. SAKAI, BARBARA E. SWARTZ and C. D. WOODY
    Neuropharmacology. 1979 Feb;18(2):209-13.
Order No. Model No. Product Description USA Price Order
64-1735 PLI-100A PLI-100A Basic Picoinjector with injection, balance, clear, filling and holding pressures. Comes with input hose, output hose, holding hose, and input hose adapter Login Login
64-1736 PLI-100A Plus PLI-100A Plus Picoinjector with injection, balance, clear, filling and holding pressures. Comes with input hose, output hose, holding hose, foot switch, pipette holder, and input hose adapter. Login Login
64-1737 PLI-100ADeluxe PLI-100A Deluxe Picoinjector with injection, balance, clear, filling and holding pressures, comes with input hose, output hose, holding hose, two foot switches, two pipette holders, and input hose adapter Login Login
Stainless Steel Pipette Holders and Parts
65-0013 PLI-PH1 Stainless Steel Pipette Holder, 130 mm L for 1.0 - 1.5 mm OD Glass Pipettes Login Login
65-0014 PLI-PH1A Stainless Steel Pipette Holder, 80 mm L for 1.0 - 1.5 mm OD Glass Pipettes Login Login
65-0017 PLI-SRG Silicone rubber gasket replacement for use with 1.0 mm glass capillaries (fits PLI-PH-1 & PLI-PH-1A holders), 10.2 cm Login Login
65-0015 PLI-SRG1.5 Silicone rubber gasket replacement for use with 1.5 mm glass capillaries (fits PLI-PH-1 & PLI-PH-1A holders), 10.2 cm Login Login
65-0018 PLI-SSB Stainless Steel Bushing Replacement for use with PLI-PH-1 and PLI-PH-1A Pipette Holders Login Login
65-0019 PLI-PC Stainless Steel Pipette Cap Replacement for use with PLI-PH-1 and PLI-PH-1A Pipette Holders Login Login
65-0020 PLI-HN Stainless Steel Hose Nut Replacement for use with PLI-PH-1 and PLI-PH-1A Pipette Holders Login Login
65-0021 PLI-PH-KIT Pipette holder hardware replacement kit for use with PLI-PH-1 and PLI-PH-1A pipette holders Login Login
65-0022 PLI-PHA Stainless Steel Pipette Holder Adapter (PLI-PH series) for Eppendorf ECET FEMTO-TIP Login Login
Acrylic Pipette Holders
64-1626 AO161.0 Acrylic pipette holder for 1.0 mm pipettes Login Login
64-1627 AO161.2 Acrylic pipette holder for 1.2 mm pipettes Login Login
64-1628 PLI-PPH Acrylic Pipette Holder for 1.5 mm pipettes Login Login
64-1629 AO162.0 Acrylic pipette holder for 2.0 mm pipettes Login Login
Accessories and Replacement Parts
64-0055 MM-33L Micromanipulator with clamp base, left handed Login Login
64-0056 MM-33R Micromanipulator with clamp base, right handed Login Login
64-0057 MM-33LT Micromanipulator with tilt base, left handed Login Login
64-0058 MM-33RT Micromanipulator with tilt base, right handed Login Login
69-0225 MB-B Magnetic base, main pole (12 mm D 176 mm L), sub pole (10 mm D 165 mm L), 800 N hold force Login Login
65-0029 PLI-FS Foot switch (compatible with PLI-100A, PLI-90A and PLI-10) for inject, hold, fill, gate Login Login
64-1701 AIR-1 Ultra low noise air compressor, 120 VAC/60Hz Login Login
65-0006 PLI-IHA Input hose adapter, 1/4 in male NPT pipe thread gas bottle regulator fitting Login Login
65-0008 PLI-IHN Gas input hose (Tygon tubing), 0.250 x 0.125 in (OD x ID), quick connect/disconnect, 9 ft Login Login
65-0010 PLI-OHN Output hose (Tygon tubing) 9 ft L x 0.071 in x 0.039 in (OD x ID), quick connect/disconnect type Login Login
65-0012 PLI-HHN Holding hose with cell release bulb, 9 ft l x 0.071 in x 0.039 in (OD x ID), quick connect/disconnect type Login Login
Legacy PLI-100 Accessories and Replacement Parts
65-0036 PLI-100 Foot switch (legacy design, compatible with PLI-100 and PLI-90, 3.5 mm mono jack) for inject, hold, fill, gate Login Login
65-0007 PLI-IHO Gas input hose (compatible with PLI-100 and PI-90,Tygon tubing), 0.250 x 0.125 in (OD x ID), brass screw fitting, 9 ft Login Login
65-0009 PLI-OHO Output hose (compatible with PLI-100 and PI-90,Tygon tubing) 9 ft L x 0.071 in x 0.039 in (OD x ID), brass screw fitting type Login Login
65-0011 PLI-HHO Holding hose (compatible with PLI-100 and PI-90)with cell release bulb, 9 ft l x 0.071 in x 0.039 in (OD x ID), brass screw fitting type Login Login

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