Specialized Tools for Electrophysiology and Cell Biology Research

(BLM) Planar Lipid Bilayer Workstation

Product Summary

The Planar Lipid Bilayer (BLM) Workstation from Warner Instruments integrates every significant component required for the assembly of a working BLM rig. This unique device allows the user to quickly get up to speed in performing research using this powerful technology.

 

  • Complete facility for Planar Lipid Bilayer recording
  • Simple and integrated design
  • Integrated instrumentation
  • Popular data acquisition packages
  • Optional power line conditioning
  • Bilayer Thermocycler available!
  • Available on-site setup and training!

The Planar Lipid Bilayer Workstation is comprised of:

  • FC Series: Faraday cage and vibration isolation table (manufactured expressly for Warner Instruments by Kinetic Systems)
  • BC-535: Bilayer Clamp Amplifier
  • LPF-8: 8-pole Bessel filter
  • SUNStir-3: Integrated system comprised of a SUNStir rack-mount controller, SUN-1 halogen lamp, and SPIN-2 bilayer stirplate
  • BPS-2: Bilayer perfusion system
  • HST-1: Headstage holder system
  • RAC-14: Table top equipment rack
  • BLM-ST: Bilayer Starter Kit

Additional items needed to complete the Workstation include bilayer cups and chambers (either the classic or perfusion style), data acquisition hardware/software, and a computer. Due to the extensive variety offered, cups and chambers are ordered separately.

The Bilayer workstation supports the use of all data acquisition systems and we offer pClamp from Molecular Devices (Union City, CA). You may purchase your acquisition system directly from the vendor or from us at no additional charge to you. (Technical support and warranty, however, remain through Molecular Devices.)

When placing an order you will need to select:

  • the style (active or passive) of vibration isolation table desired
  • the style (classic or perfusion) of bilayer chamber desired
  • the material (polystyrene, Delrin, or polysulfone) of bilayer cup desired
  • the diameter (150, 200, 250 µm, or custom) of the cup aperture desired

Additional optional items include:

BLM-TC - Planar Lipid Bilayer Thermocycler. Temperature control for the bilayer!
On-site setup and training - Available worldwide.
ZM-186 -Stereo zoom microscope for viewing the membrane
CM-3 - A variable value single channel simulator.
PV-830 - Power line conditioner available for both 110 and 220 V applications
Starter Kit - Includes red sable artist's dotting brushes, glass capillary tubing, PE tubing, Ag/AgCl wire, BNC's, grounding cables, micro stirbars, and rubber matting for traction control of the bilayer chamber.

A fully assembled Planar Lipid Bilayer Workstation from Warner Instruments includes
everything you need, except the computer, for performing research using this powerful technology.

Order No. Model No. Product Description USA Price Order
64-0450 BLM-TC Bilayer thermocycler (requires bilayer chamber and cup) Login Login
64-0455 PV-830 Power Line Conditioner 1000 VA, 120 VAC 60HZ Login Login
64-0456 PV-830CE Power Line Conditioner 1000 VA, 220 VAC 50/60HZ Login Login
64-0451 BCH-M13 Bilayer chamber, 1.0 ml working volume, with stirbar, without cuvette Login Login
64-0025 CM-1/100 Single channel simulator, fixed characteristics, 100 pF capacitance Login Login
64-0027 CM-3/100 Single channel simulator, variable characteristics, 100 pF capacitance Login Login
64-0071 ZM186 Stereo zoom microscope Login Login

References

 

  1. Vesicle fusion to planar membranes is enhanced by cholesterol and low temperature 
    Lee DE, Lew MG, Woodbury DJ
    Chem Phys Lipids. 2013 Jan;166:45-54. doi: 10.1016
  2. Model-based prediction of the alpha-hemolysin structure in the hexameric state 
    Furini S, Domene C, Rossi M, Tartagni M, Cavalcanti S
    Biophys J. 2008 Sep;95(5):2265-74. Epub 2008 May 23
  3. Synthesis-enabled functional group deletions reveal key underpinnings of amphotericin B ion channel and antifungal activities 
    Palacios DS, Dailey I, Siebert DM, Wilcock BC, Burke MD 
    Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6733-8
  4. Cys-Cys cross-linking shows contact between the N-terminus of lethal factor and Phe427 of the anthrax toxin pore 
    Janowiak BE, Jennings-Antipov LD, Collier RJ 
    Biochemistry. 2011 May 3;50(17):3512-6
  5. Identification and experimental verification of a novel family of bacterial cyclic nucleotide-gated (bCNG) ion channels 
    Caldwell DB, Malcolm HR, Elmore DE, Maurer JA 
    Biochim Biophys Acta. 2010 Sep;1798(9):1750-6
  6. The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel 
    Sheppard DN, Gray MA, Gong X, Sohma Y, Kogan I, Benos DJ, Scott-Ward TS, Chen JH, Li H, Cai Z, Gupta J, Li C, Ramjeesingh M, Berdiev BK, Ismailov II, Bear CE, Hwang TC, Linsdell P, Hug MJ
    J Cyst Fibros. 2004 Aug;3 Suppl 2:101-8
  7. Photopolymerization of mixed monolayers and black lipid membranes containing gramicidin A and diacetylenic phospholipids 
    Daly SM, Heffernan LA, Barger WR, Shenoy DK
    Langmuir. 2006 Jan 31;22(3):1215-22
  8. Differentially distributed IP3 receptors and Ca2+ signaling in rod bipolar cells 
    Koulen P, Wei J, Madry C, Liu J, Nixon E
    Invest Ophthalmol Vis Sci. 2005 Jan;46(1):292-8
  9. Hydroxylated Xestospongins Block Inositol-1,4,5-trisphosphate-Induced Ca2+ Release and Sensitize Ca2+-Induced Ca2+ Release Mediated by Ryanodine Receptors 
    Ta TA, Feng W, Molinski TF, Pessah IN
    Mol Pharmacol. 2006 Feb;69(2):532-8
  10. Structural and functional characterization of the nitrite channel NirC from Salmonella typhimurium 
    Lü W, Schwarzer NJ, Du J, Gerbig-Smentek E, Andrade SL, Einsle O
    Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18395-400
  11. ORF8a of SARS-CoV forms an ion channel: Experiments and molecular dynamics simulations 
    Chen CC, Krüger J, Sramala I, Hsu HJ, Henklein P, Chen YM, Fischer WB
    Biochim Biophys Acta. 2011 Feb;1808(2):572-9. doi: 10.1016
  12. Three reversible and controllable discrete steps of channel gating of a viral DNA packaging motor 
    Geng J, Fang H, Haque F, Zhang L, Guo P
    Biomaterials. 2011 Nov;32(32):8234-42. doi: 10.1016
  13. An involvement of yeast peroxisomal channels in transmembrane transfer of glyoxylate cycle intermediates 
    Antonenkov VD, Mindthoff S, Grunau S, Erdmann R, Hiltunen JK
    Int J Biochem Cell Biol. 2009 Dec;41(12):2546-54. doi: 10.1016
  14. Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2 
    Anyatonwu GI, Estrada M, Tian X, Somlo S, Ehrlich BE
    Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6454-9
  15. Methanethiosulfonate ethylammonium block of amine currents through the ryanodine receptor reveals single pore architecture 
    Anyatonwu GI, Buck ED, Ehrlich BE
    J Biol Chem. 2003 Nov 14;278(46):45528-38
  16. Channelforming activities of peroxisomal membrane proteins from the yeast Saccharomyces cerevisiae 
    Grunau S, Mindthoff S, Rottensteiner H, Sormunen RT, Hiltunen JK, Erdmann R, Antonenkov VD
    FEBS J. 2009 Mar;276(6):1698-708. doi: 10.1111
  17. Self-assembling subnanometer pores with unusual mass-transport properties 
    Zhou X, Liu G, Yamato K, Shen Y, Cheng R, Wei X, Bai W, Gao Y, Li H, Liu Y, Liu F, Czajkowsky DM, Wang J, Dabney MJ, Cai Z, Hu J, Bright FV, He L, Zeng XC, Shao Z, Gong B
    Nat Commun. 2012 Jul 17;3:949. doi: 10.1038
  18. Pxmp2 Is a Channel-Forming Protein in Mammalian Peroxisomal Membrane 
    Rokka A, Antonenkov VD, Soininen R, Immonen HL, Pirilä PL, Bergmann U, Sormunen RT, Weckström M, Benz R, Hiltunen JK
    PLoS One. 2009;4(4):e5090. Epub 2009 Apr 7
  19. Effect of 2-Fluorohistidine Labeling of the Anthrax Protective Antigen on Stability, Pore Formation, and Translocation 
    Wimalasena DS, Cramer JC, Janowiak BE, Juris SJ, Melnyk RA, Anderson DE, Kirk KL, Collier RJ, Bann JG
    Biochemistry. 2007 Dec 25;46(51):14928-36. Epub 2007 Nov 29
  20. Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion 
    de Planque MR, de Planque MR, Mendes GP, Zagnoni M, Sandison ME, Fisher KH, Berry RM, Watts A, Morgan H 
    IEE Proc Nanobiotechnol. 2006 Apr;153(2):21-30

Order No. Model No. Product Description USA Price Order
64-0452A BLM-WS-A Planar Lipid Bilayer Workstation with Active Table Login Login
64-0452P BLM-WS-P Planar Lipid Bilayer Workstation with Passive Table Login Login
98-6131 pClamp pClamp 11 Standard software, required for use with DigiData Login Login
98-5692 1550B1 DigiData 1550B digitizer, HumSilencer capability on 1 channel Login Login
64-0450 BLM-TC Bilayer thermocycler (requires bilayer chamber and cup) Login Login
64-0455 PV-830 Power Line Conditioner 1000 VA, 120 VAC 60HZ Login Login
64-0456 PV-830CE Power Line Conditioner 1000 VA, 220 VAC 50/60HZ Login Login
64-1835 Binocular stereo microscope, 8-50x zoom, ball bearing boom stand Login Login
64-0405 CP22A-250 Polystyrene cuvette, 3.0 ml working volume, with 250 µm aperture Login Login
64-0418 CF22A-200 Polysulfone cuvette, 3.0 ml working volume, with 200 µm aperture Login Login
Optional Items and Accessories
64-0083 OST-1 Onsite setup and training, per day (2 days minimum, add travel) Login Login
Cups and Chambers - Classic 13 mm
64-0451 BCH-M13 Bilayer chamber, 1.0 ml working volume, with stirbar, without cuvette Login Login
64-0404 CP13A-150 Polystyrene cuvette, 1.0 ml working volume, with 150 µm aperture Login Login
64-0403 CP13A-200 Polystyrene cuvette, 1.0 ml working volume, with 200 µm aperture Login Login
64-0402 CP13A-250 Polystyrene cuvette, 1.0 ml working volume, with 250 µm aperture Login Login
64-0410 CD13A-150 Delrin cuvette, 1.0 ml working volume, with 150 µm aperture Login Login
64-0409 CD13A-200 Delrin cuvette, 1.0 ml working volume, with 200 µm aperture Login Login
64-0408 CD13A-250 Delrin cuvette, 1.0 ml working volume, with 250 µm aperture Login Login
64-0416 CF13A-150 Polysulfone cuvette, 1.0 ml working volume, with 150 µm aperture Login Login
64-0415 CF13A-200 Polysulfone cuvette, 1.0 ml working volume, with 200 µm aperture Login Login
64-0414 CF13A-250 Polysulfone cuvette, 1.0 ml working volume, with 250 µm aperture Login Login
Cups and Chambers - Classic 22 mm
64-0453 BCH-M22 Bilayer chamber, 3.0 ml working volume, with stirbar, without cuvette Login Login
64-0407 CP22A-150 Polystyrene cuvette, 3.0 ml working volume, with 150 µm aperture Login Login
64-0406 CP22A-200 Polystyrene cuvette, 3.0 ml working volume, with 200 µm aperture Login Login
64-0413 CD22A-150 Delrin cuvette, 3.0 ml working volume, with 150 µm aperture Login Login
64-0412 CD22A-200 Delrin cuvette, 3.0 ml working volume, with 200 µm aperture Login Login
64-0411 CD22A-250 Delrin cuvette, 3.0 ml working volume, with 250 µm aperture Login Login
64-0419 CF22A-150 Polysulfone cuvette, 3.0 ml working volume, with 150 µm aperture Login Login
64-0417 CF22A-250 Polysulfone cuvette, 3.0 ml working volume, with 250 µm aperture Login Login
Cup and Chamber Accessories
64-0420 MAG-13 Stir Bars, 2 x 5mm for 1 ml cuvettes, 5 pack Login Login
64-0421 MAG-22 Stir Bars, 2 x 7mm for 3 ml cuvettes, 5 pack Login Login
64-1729 BCH-13TH Thumb screw and rubber plug for BCH-M13 and BCH-M22 chambers Login Login
Workstation Components
64-0064 FC-2 Bilayer Faraday cage with Dynamic isolation table Login Login
64-0432 BC-535 Bilayer clamp amplifier with resistive headstage Login Login
64-0050 LPF-8 Low pass bessel filter, 8-pole, with differential amplifier, 110-240 VAC, 50/60 Hz Login Login
64-0076 SUNSTIR-3 SUNStir-3 Bundle (Spin-2, SUN-1, SUNStir Controller) Login Login
64-0431 BPS-2 Bilayer perfusion system Login Login
64-0435 HST-1 Headstage holder system for bilayer Login Login
64-0070 RAC-14 14" Tabletop Instrument Rack Login Login
64-0067 BLM-ST Bilayer starter kit Login Login

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