Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-28) Excised Patch Chamber with Two Slotted Open Baths
Product Summary
The dual slotted open bath construction of the excise patch chamber permits exchange of solution surrounding a membrane patch without contamination of the associated cell population.
- Designed for excised patch studies
- Open chamber design
- Dual slotted baths in two sizes
- Baths separated by a controllable dam
The RC-28 is a special chamber containing two bath compartments separated by an adjustable dam. The dam functions both as a gate and barrier between the baths. The composition and flow rate of each bath can be independently controlled, and each bath has a separate stainless steel suction tube for perfusion.
The dual slotted, open bath design allows for the exchange of solution surrounding an isolated membrane patch without contaminating or modifying the rest of the population. Raising the solution level in the dam area joins the two baths, while lowering the solution level isolates the two baths. After patching onto a cell in the large bath compartment, the dam is flooded, enabling secure transfer of the electrode attached cell to the smaller compartment. The dam is then drained isolating the two baths.
For the larger chamber, the bath area is 21 x 9.5 mm and the working volume is 243 ul per 1.0 mm of solution height. For the smaller chamber, the bath area is 28 x 3.8 mm and the volume is 111 ul.
A platform (PM-6, PH-6 or P-6) and Series 20 stage adapter are required to complete the assembly for mounting onto a microscope. The chamber bottom is formed by a 24 x 50 mm coverslip.
The RC-28 is supplied with a pack of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, 50/pkg) and 10 ft of PE-160 polyethylene tubing.
| Material | Polycarbonate |
|---|---|
| Dimensions, small bath d (L x W x H) |
20.0 x 3.8 x 4.5 mm |
| Dimensions, large bath d (L x W x H) |
28.0 x 9.5 x 4.5 mm |
| Volume, small bath, by depth | 111 µl |
| Volume, large bath, by depth | 243 µl |
| Specimen area, small bath (L x W) | 28 x 3.8 mm |
| Specimen area, large bath (L x W) | 21 x 9.5 mm |
| Minimum access angle (from side to center of large bath) | 40° |
| Bottom coverglass | 24 x 50 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
References
References
- Barium, TEA and sodium sensitive potassium channels are present in the human placental syncytiotrophoblast apical membrane
Díaz P, Vallejos C, Guerrero I, Riquelme G.
Placenta. 2008 Oct;29(10):883-91 - The maxi-chloride channel in human syncytiotrophoblast: a pathway for taurine efflux in placental volume regulation
Vallejos C, Riquelme G
Placenta. 2007 Nov-Dec;28(11-12):1182-91 - Correlative Studies of Gating in Cx46 and Cx50 Hemichannels and Gap Junction Channels
Miduturu Srinivas, Jack Kronengold, Feliksas F. Bukauskas,y Thaddeus A. Bargiello, and Vytas K. Verselisy
Biophysical Journal Volume 88 March 2005 1725–1739
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chamber | ||||
| 64-0243 | RC-28 | Excised patch recording chamber | Login | Login |
| Platforms | ||||
| 64-1527 | PM-6 | PM-6 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0289 | PH-6 | PH-6 Platform for Series 20 chambers, heater | Login | Login |
| 64-0282 | P-6 | P-6 Platform for Series 20 chambers, non-heater | Login | Login |
| Replacement Consumables | ||||
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-3018 | PM-plate | Pressure plates for PM Series platforms, 2 pack | Login | Login |
| 64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |