Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-30HV, RC-30WA) Confocal Imaging Chambers
Product Summary
The RC-30 Series are low profile, closed bath chambers specifically designed for confocal imaging applications. Compatible with either upright or inverted microscopes.
- Option of confocal imaging with continuous perfusion
- Accommodates both upright and inverted microscopes
- 17.7 mm diameter viewing aperture (RC-30HV, RC-30WA)
- 25.0 mm diameter viewing aperture (RC-30WA)
- User defined cross-sectional viewing area
- User defined bath volume with fast fluid exchange
- Three available gasket thicknesses
- Resisitive heating on chamber base
In both designs, #1 thickness glass coverslips form the chamber top and bottom, creating the closed bath. The closed design allows the chamber to be inverted in the mounting platform enabling its use with either inverted or upright microscopes.
The RC-30HV uses a 22x30 mm coverslip for the chamber top and a 22x40 mm coverslip for the bottom. The final viewing aperture is 17.7 mm.
The RC-30WA is a wide aperture version of the RC-30HV. It uses a 40 mm round coverslip for the bottom surface and a 30 mm round coverslip for the top. The final viewing aperture for this chamber is 25 mm.
Variable Bath Volume/Fast Perfusate Exchange
The side walls of the RC-30HV and RC-30 WA bath are formed by a silicone gasket sandwiched between the top and bottom coverslips. Wall height (i.e. spacing between the two coverslips) can range from 125-1000 µm and is defined by the thickness and number of gaskets used. Pre-cut, as well as blank gaskets are supplied with the chamber allowing the development of customized bath geometries as needed.
The supplied pre-cut gaskets include a wide cut gasket to allow for the full use of the aperture window (17.7 mm diameter) and a narrow, slotted version for fast solution exchange. The bath volumes for pre-cut, 250 µm thick gaskets are 133 µl for the wide cut and 26 µl for the slotted cut.
Large Viewing Area
The viewing aperture in the chamber housing is 17.7 mm in diameter. The chamber bottom is beveled to permit maximum access to the bottom coverslip.
Reversible
The RC-30 Series chambers can be mounted right-side up or up-side down in the stage adapter to accommodate use on either upright and inverted microscopes.
Heating
The RC-30HV and RC-30WA include a pair of resistive heating elements mounted to the chamber base. These elements are compatible with Warner's TC-344C and TC-324C temperature controllers which are ordered separately along with a CC-28 cable.All versions of the RC-30 chambers require special Series 30 microscope stage adapters which also must be ordered separately.
Confocal imaging chambers are supplied with six packages of pre-cut gaskets: two large area bath gaskets (GS-30L/10, GS-30L/15), two slotted bath gaskets (GS-30S/10), and two blank gaskets (GS-30B/10, GS-30B/15) for user customized chamber areas. Also included is a special tool used for mounting the top plate, polyethylene tubing (PE-90/10, 10 feet.), and 22x30 mm and 22x40 mm #1 glass coverslips (CS-22/30 and CS-22/40, pkg. of 50). The RC-30WA includes one box each of 30 mm round coverslips, (CS-30R, and 40 mm round coverslips, CS-40R). Warner offers 125, 250, and 375 µm thick gaskets which are listed in the order information section.
See Related Files to download a data sample collected using the RC-30 chamber (47 MB)
| Physical Dimensions (H X D) | 9.5 x 82 mm |
|---|---|
| Weight | <90 g, assembled with optional heaters |
| Base and Pressure Plate | Aluminum, black anodized |
| Top Coverslip Plate | Polycarbonate |
| Gaskets | Silicone rubber |
| Input/Output Tubing | PE-90 |
| Coverslips (#1) | Top: 22 x 30 mm; Bottom: 22 x 40 mm |
| Microscope Stage Adapters* | All major models: Leica, Nikon, Zeiss, Olympus, Prior, Ludl, Burleigh |
*Note: Uses Series 30 stage adapters.
For exterior dimensions use exterior dimension information for corresponding Series 20 adapters.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0754 | PE-90/10 | Polyethylene tubing, PE-90, 10 ft | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0102 | SH-27B | In-line solution heater | Login | Login |
| 64-0103 | SF-28 | Slow flow in-line solution heater | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Resonant Rayleigh light scattering response of individual Au nanoparticles to antigen–antibody interaction
Cao C, Sim SJ
Lab Chip. 2009 Jul 7;9(13):1836-9 - Elevated hydrostatic pressure activates sodium-hydrogen exchanger-1 in rat optic nerve head astrocytes
Mandal A, Shahidullah M, Delamere NA, Terán MA
Am J Physiol Cell Physiol. 2009 Jul;297(1):C111-20 - Sodium/bicarbonate cotransporter NBCn1/slc4a7 increases cytotoxicity in magnesium depletion in primary cultures of hippocampal neurons
Cooper DS, Yang HS, He P, Kim E, Rajbhandari I, Yun CC, Choi I
Eur J Neurosci. 2009 Feb;29(3):437-46 - Silencing of ZnT1 reduces Zn2+ efflux in cultured cortical neurons
Qin Y, Thomas D, Fontaine CP, Colvin RA
Neurosci Lett. 2009 Jan 30;450(2):206-10 - Mechanisms of Zn2+ efflux in cultured cortical neurons
Qin Y, Thomas D, Fontaine CP, Colvin RA
J Neurochem. 2008 Dec;107(5):1304-13 - Insights into Zn2+ homeostasis in neurons from experimental and modeling studies
Colvin RA, Bush AI, Volitakis I, Fontaine CP, Thomas D, Kikuchi K, Holmes WR
Am J Physiol Cell Physiol. 2008 Mar;294(3):C726-42 - Tonic Shock Induces Detachment of Giardia lamblia
Hansen WR, Fletcher DA.
PLoS Negl Trop Dis. 2008 Feb 13;2(2):e169 - The role of Akt in the signaling pathway of the glycoprotein Ib-IX–induced platelet activation
Yin H, Stojanovic A, Hay N, Du X
Blood. 2008 Jan 15;111(2):658-65 - Endothelial Targeting of High-Affinity Multivalent Polymer Nanocarriers Directed to Intercellular Adhesion Molecule 1
Silvia Muro, Thomas Dziubla, Weining Qiu, John Leferovich, Xiumin Cui, Erik Berk, and Vladimir R. Muzykantov
JPET 317:1161–1169, 2006 - Acute Glutathione Depletion Restricts Mitochondrial ATP Export in Cerebellar Granule Neurons
Sabino Vesce1, Mika B. Jekabsons, Linda I. Johnson-Cadwell, and David G. Nicholls
J. Biol. Chem., Vol. 280, Issue 46, 38720-38728, November 18, 2005
Related Files
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0321 | RC-30HV | RC-30HV confocal imaging chamber, with heaters | Login | Login |
| 64-0321WA | RC-30WA | RC-30WA wide aperture confocal imaging chamber | Login | Login |
| Replacement Gaskets | ||||
| 64-0342 | GS-30L/4 | Large bath gasket, 125 µm thick, 10 pack | Login | Login |
| 64-0330 | GS-30L/10 | Large bath gasket, 250 µm thick, 10 pack | Login | Login |
| 64-0331 | GS-30L/15 | Large bath gasket, 375 µm thick, 10 pack | Login | Login |
| 64-0343 | GS-30S/4 | Slotted bath gasket, 125 µm thick, 3.2 mm slot, 10 pack | Login | Login |
| 64-0332 | GS-30S/10 | Slotted bath gasket, 250 µm thick, 3.2 mm slot, 10 pack | Login | Login |
| 64-0333 | GS-30S/15 | Slotted bath gasket, 375 µm thick, 3.2 mm slot, 10 pack | Login | Login |
| 64-0341 | GS-30B/4 | Blank gasket, 125 µm thick, 10 pack | Login | Login |
| 64-0334 | GS-30B/10 | Blank gasket, 250 µm thick, 10 pack | Login | Login |
| 64-0335 | GS-30B/15 | Blank gasket, 375 µm thick, 10 pack | Login | Login |
| Replacement Consumables | ||||
| 64-0706 | CS-22/30 | Rectangular cover glass, #1 thickness, 22 x 30 mm, 100 pack | Login | Login |
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-1500 | CS-40R | Round cover glass, #1 thickness, 40 mm, 50 pack | Login | Login |
| 64-1499 | CS-30R15 | Round cover glass, #1.5 thickness, 30 mm, 90 pack | Login | Login |
| 64-0754 | PE-90/10 | Polyethylene tubing, PE-90, 10 ft | Login | Login |
| Heating Accessories | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0102 | SH-27B | In-line solution heater | Login | Login |
| 64-0103 | SF-28 | Slow flow in-line solution heater | Login | Login |
| Replacement Parts | ||||
| 64-1464 | RC-30-TCP | RC-30 top coverslip plate replacement | Login | Login |
| 64-1464WA | RC-30WA-TCP | RC-30WA top coverslip plate replacement | Login | Login |
| 64-1559 | RH-3 | Replacement heater elements for Series 30 chambers, 2 pack | Login | Login |