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Self-Contained Ussing Systems
Product Summary
The U9926 and U2500 Self-Contained Ussing chambers improve on the Classic design by bringing the solution reservoir into the chamber housing reducing the apparatus size and increasing ease-of-use
- Modular design with separate inserts
- Supports membranes from 3.8 to 13.5 mm wide
- No glass circulation reservoirs
- Supports Snapwell, Transwell, and Millicell culture cups
- Chamber heating is provided by integral water jackets
- Compatible with UssingChart hardware and software
The Self-Contained Ussing Chambers systems come in single- and dual-channel models and offer many advantages over the Classic design. The single-channel U9926 and dual-channel U2500 are shown above and to the right. Both systems make use of inserts to secure and position the tissue or culture cup within the chamber body.
Chamber bodies (single- and dual-chamber models) consist of chamber-halves which clamp around the insert(s). Each chamber-half has four ports that enter into its respective bath compartment: a voltage electrode port, a current electrode port, an air/gas port, and a solution drain port. Solution warming is provided by pumping heated water through each chamber-half and there are no delicate glass heat exchangers to be broken. The dual-chamber U2500 has the additional feature of including four needle valves for adjusting the air/gas mixture into each individual chamber-half.
Inserts can be easily exchanged between experiments. Solutions are removed from the chambers through the drain valves and the halves unclamped from each other. The insert is then simply replaced. Inserts are available to support a variety of tissue sizes and culture cups.
The U9926 (single-channel chamber is supplied with 1 electrode set (consisting of 2 voltage and 2 current electrodes), 12 bridge fittings with adapters; and a tubing/ fitting kit. The U2500 (dual channel) chamber is supplied with two each of the above described accessories. Chamber inserts are ordered separately.
Data Acquisition
The Self-Contained Ussing systems are compatible with electrophysiological epithelial transport measurements and use UssingChart data acquisition. This package includes an amplifier, a digitizer, software, and cables. You can view details on the UssingChart Data Acquisition webpage.
Self-Contained Ussing Chamber System and DAQ Expandability
This self-contained Ussing system can be scaled by adding chambers and expanding the UssingChart DAQ.
The Pricing and Ordering tab of this page lists the Chamber Systems and the 2 Channel UssingChart Data Acquisition System.
Information regarding increasing the number of DAQ channels can be found on the UssingChart Data Acquisition webpage.
Working volume is 5-8 ml depending on insert.
For all chambers not using culture cups:
Increase the tissue dimensions by a minimum of 3.0 mm in each direction
to allow the o-ring or pins to capture the tissue without tearing.
For example, a chamber with a 5 mm round aperture will require a tissue with a minimum diameter of 8 mm,
and a chamber with a 2 x 10 mm oblong aperture will require a tissue with minimum dimension of 5 x 13 mm.
Suggested application(s) by aperture
| Aperture | Applications | Aperture area |
|---|---|---|
| 3.8 mm round with o-ring | biopsy soft tissue |
0.11 cm2 |
| 6 mm round with o-ring | skin and soft tissue general use |
0.28 cm2 |
| 9 mm round with o-ring | skin and soft tissue general use |
0.64 cm2 |
| 12 mm round with o-ring | skin and soft tissue cornea general use |
1.13 cm2 |
| 13.5 mm round with o-ring | skin cornea general use |
1.43 cm2 |
| 3.8 mm round with pins | general use | 0.11 cm2 |
| 6 mm round with pins | general use | 0.28 cm2 |
| 9 mm round with pins | general use | 0.64 cm2 |
| 12 mm round with pins | skin general use |
1.13 cm2 |
| 13.5 mm round with pins | skin general use |
1.43 cm2 |
| 1.6 x 7 mm oblong with pins | mouse intestine general use |
0.11 cm2 |
| 2.4 x 7 mm oblong with pins | mouse intestine general use |
0.17 cm2 |
| 3.5 x 7 mm oblong with pins | mouse intestine rat intestine general use |
0.25 cm2 |
| Snapwell, 12.7 mm | tissue culture | 1.12 cm2 |
| Millicell, 12 mm | tissue culture | 1.13 cm2 |
| Transwell, 6.5 mm | tissue culture | 0.33 cm2 |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0565 | U9406 | Tubing kit (hose and Luer fittings) | Login | Login |
| 64-0554 | U9975A | Electrode set, includes 2 Ag/AgCl pellets and 2 Ag wires | Login | Login |
| 64-1521 | U9565SC | Electrode bridge fitting kit (pipette tips and adapters), 12 pack | Login | Login |
| 64-0555 | U9985 | Bridge fitting adapters, 6 pack | Login | Login |
| 64-0604 | U9605 | O-rings for U9926 and U2500 Ussing chambers, 4 pack | Login | Login |
References
- Extracellular calcium- and magnesium-mediated regulation of passive calcium transport across Caco-2 monolayers
Davies SL, Gibbons CE, Steward MC, Ward DT
Biochim Biophys Acta. 2008 Oct;1778(10):2318-24
Related Files
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
Pricing Top Description
How to order:
- Select Base Assembly
Each system includes one or two U9975A Electrode Set(s) and U9965EP Electrode Bridge Fitting Kit(s)
Order 1 pack of filling needles. - Select Insert
Order 1 if using the U9926 and order 2 if using the U2500 - Select Data Acquisition package for Ussing (UssingChart)
If making electrophysiological measurements - Select Thermocirculator and Tubing Kit
If needed - for temperature control
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Base Assembly | ||||
| 64-0575S | U2500 | Dual-channel Ussing chamber base assembly, does not include inserts | Login | Login |
| 64-0568S | U9926 | Single-channel Ussing chamber base assembly, does not include insert | Login | Login |
| 75-0286 | Stainless steel filing needles for Ussing chambers, pack of 6 | Login | Login | |
| Inserts for tissue with pins | ||||
| 64-0587 | U9924B-04 | Tissue insert with pins, 3.8 mm aperture, 0.11 cm2 area | Login | Login |
| 64-0588 | U9924B-06 | Tissue insert with pins, 6.0 mm aperture, 0.28 cm2 area | Login | Login |
| 64-0590 | U9924B-12 | Tissue insert with pins, 12.0 mm aperture, 1.13 cm2 area | Login | Login |
| 64-0591 | U9924B-13 | Tissue insert with pins, 13.5 mm aperture, 1.43 cm2 area | Login | Login |
| 64-0592 | U9924C-03 | Tissue insert with pins, 1.6 x 7.0 mm aperture, 0.11 cm2 area | Login | Login |
| 64-0593 | U9924C-04 | Tissue insert with pins, 2.4 x 7.0 mm aperture, 0.17 cm2 area | Login | Login |
| 64-0594 | U9924C-05 | Tissue insert with pins, 3.5 x 7.0 mm aperture, 0.25 cm2 area | Login | Login |
| Inserts for tissue with o-ring | ||||
| 64-0582 | U9924A-04 | Tissue insert with o-ring, 3.8 mm aperture, 0.11 cm2 area | Login | Login |
| 64-0583 | U9924A-06 | Tissue insert with o-ring, 6.0 mm aperture, 0.28 cm2 area | Login | Login |
| 64-0584 | U9924A-09 | Tissue insert with o-ring, 9.0 mm aperture, 0.64 cm2 area | Login | Login |
| 64-0585 | U9924A-12 | Tissue insert with o-ring, 12.0 mm aperture, 1.13 cm2 area | Login | Login |
| 64-0586 | U9924A-13 | Tissue insert with o-ring, 13.5 mm aperture, 1.43 cm2 area | Login | Login |
| Inserts for culture cups | ||||
| 64-0600 | U9924S | Culture cup insert for Snapwell, 12.7 mm, 1.12 cm2 area | Login | Login |
| 64-0601 | U9924T-06 | Culture cup insert for Transwell adapter 6.5 mm, 0.33 cm2 area | Login | Login |
| 64-0598 | U9924M-12 | Culture cup insert for Millicell adapter, 12.0 mm, 1.13 cm2 area | Login | Login |
| Thermocirculator | ||||
| 72-4924 | TC-120 | Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz | Login | Login |
| 72-4921 | TC-120 | Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz | Login | Login |
| 64-2244 | WB-KIT | TC-120 Thermocirculator Tubing Kit | Login | Login |
|
2 Channel UssingChart Package
- order 1 of each |
||||
| 89-5285 | UssingChart software | Login | Login | |
| 64-1605 | EC-825A | Dual channel epithelial voltage clamp, ±50V compliance, 110-250 VAC | Login | Login |
| 89-5035 | LIH 8+8 | LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE | Login | Login |
| 64-3008 | EC-825A cable, single - fits 1 amplifier (2 acquisition channels) | Login | Login | |
| Electrodes and Components | ||||
| 64-0554 | U9975A | Electrode set, includes 2 Ag/AgCl pellets and 2 Ag wires | Login | Login |
| 64-1521 | U9565SC | Electrode bridge fitting kit (pipette tips and adapters), 12 pack | Login | Login |
| 64-0555 | U9985 | Bridge fitting adapters, 6 pack | Login | Login |
| 64-0554P | Voltage electrodes, Ag/AgCl pellet, 2 mm pin, 4 pack | Login | Login | |
| 64-0554W | Current electrodes, Ag wires, 2 mm pin, 4 pack | Login | Login | |
| Replacement Parts | ||||
| 75-0286 | Stainless steel filing needles for Ussing chambers, pack of 6 | Login | Login | |
| 64-0604 | U9605 | O-rings for U9926 and U2500 Ussing chambers, 4 pack | Login | Login |
| 64-0567 | SL-6/U | Stopcock with luer fitting for U9926 and U2500, 6 pack | Login | Login |
| 64-0565 | U9406 | Tubing kit (hose and Luer fittings) | Login | Login |