The BSC-ZT Zbicz Top is a submersion-type chamber in which tissue slices are supported on a stiff nylon mesh. Slices are perfused from below and above.
The Zbicz Top is a submersion-type chamber in which tissue slices are supported on a stiff nylon mesh. Slices are perfused both from below and above.
Slices remain viable for 10 hours or more
The Zbicz Top (BSC-ZT) is a submersion-type chamber in which tissue slices are supported on a nylon mesh. The perfusate flows transversely across both cut surfaces. The steady flow of warmed media across the slightly submerged slice surfaces is sufficient to keep many tissues alive and responsive for 10 or more hours.
The fluid level is set to 1 mm above the mesh by a dam at the end of the chamber. The chamber is 6 mm wide and the trough is 24 mm long; this provides adequate room for slices and instrumentation, yet minimizes the well volume to optimize drug change over times. Fluid overflowing the dam is drained via a vacuum line to waste.
Slices are mechanically stable
Slices are placed on a special ribbed nylon mesh and may be held in place by 3 to 4 stimulating electrodes or a plastic insert. This stability is attributed to the stiff nylon mesh which does not rise or sag with changes in fluid flow, therefore moderate changes in the transverse laminar flow can be well tolerated during intracellular and extracellular recordings. These features allow for switching of solutions with minimal risk of tissue movement and loss of neurons while recording.
Low volume chamber
The low volume of the chamber cavity and the relatively fast flow rate allows rapid exchange of solution (1 to 2 minutes) which facilitates drug application studies.
Slices remain moist
Slices remain moist and viable, because they are wetted on both sides and do not dry out due to surrounding air currents.
Semipermanent mesh
The mesh surface holding the slice is semipermanent and can be cleaned easily. The BSC-ZT comes with extra mesh which can be installed easily when necessary.
Low electrode capacitance
Because the fluid depth is shallow (400 to 600 microns), the electrode capacitance remains low; an important consideration in single microelectrode voltage and patch clamping.
Minimal bubble formation
Bubbles, which can form in the heating unit, escape by the path formed at the entrance of the trough. Movement of the slice will not result if bubbles inadvertently form under the mesh. The flow of solution over the slice top will maintain tissue viability.
Dimensions, (L x W x H) | 98.55 x 101.60 x 28.19 mm (3.880 x 4.000 x 1.110 in) |
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Weight | 124.74 g (4.40 oz) |
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
Order No. | Model No. | Product Description | USA Price | Order |
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Warner Based System | ||||
64-1525 | BSC-BUW | BSC-BU base unit compatible with Warner temperature controllers, includes connecting cable | Login | Login |
65-0074 | BSC-ZT | Zbicz Top including nylon mesh | Login | Login |
65-0075 | BSC-HT | Haas top including 2 slice holders | Login | Login |
65-0076 | BSC-PC | Prechamber for holding slices | Login | Login |
Med Systems Based System | ||||
65-0074 | BSC-ZT | Zbicz Top including nylon mesh | Login | Login |
65-0075 | BSC-HT | Haas top including 2 slice holders | Login | Login |
65-0076 | BSC-PC | Prechamber for holding slices | Login | Login |
Accessories and Replacement Parts (General) | ||||
65-0078 | BSC-AIR | Aeration Element Replacement, Set of 2 | Login | Login |
65-0081 | BSC-TUBE | Base Unit (BSC-BU) tubing set replacement, Tygon 0.031 x 0.093 (ID x OD) | Login | Login |
65-0079 | BSC-ORING | Base Unit (BSC-BU) replacement gasket set | Login | Login |
65-0082 | BSC-G10 | Haas top (BSC-HT) tissue insert replacement, Set of 2 | Login | Login |
65-0083 | BSC-HTC | Haas top (BSC-HT) plastic cover replacement | Login | Login |
65-0084 | BSC-HT-AG | Haas top (BSC-HT) ground wire replacement | Login | Login |
65-0086 | BSC-ZTC | Zbicz Top (BSC-ZT) plastic insert replacement | Login | Login |
65-0087 | BSC-PC-VAL | Prechamber (BSC-PC) valve replacement | Login | Login |
65-0608 | BSC-PC Insert | Insert for BSC-PC Prechamber Tissue Slice Chamber | Login | Login |
65-0090 | BSC-MESH | Replacement mesh for Haas and Zbicz tops, and for prechamber cup bottom, 4 x 4 in | Login | Login |
65-0088 | BSC-ACC-KIT | Base Unit, Haas and Zbicz tops accessory kit (replacement screws, fittings and mesh) | Login | Login |
Accessories and Replacement Parts (Warner) | ||||
64-1697 | BSC-HEW | Replacement heating wire for BSC-BUW | Login | Login |
64-1591 | BSC-CABLE-W | BSC-BUW connecting cable for Warner temperature controllers | Login | Login |
Accessories and Replacement Parts (Med Systems) | ||||
65-0080 | BSC-HE | Replacement heating wire for BSC-BU | Login | Login |
65-0091 | BSC-CABLE | BSC-BU connecting cable for Med Systems temperature controllers | Login | Login |
65-0104 | Output cable, 6 pin to banana for TC-202A or BSC-BU | Login | Login |