Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-43C) Closed Bath Chamber
Product Summary
Designed for multiple successive experiments requiring the fast exchange of #1 or #1.5 round coverslips.
- Dual O-ring seal system enables quick coverslip exchange that's ideal for rapid screening assays
- Closed bath design promotes both smooth continuous solution exchange and a stable imaging focus
- Anodized aluminum base insures good heat transfer
Warner Instruments is pleased to introduce a new family of chambers designed to meet the need for rapid screening of cells grown on coverslips. The innovative design uses O-rings to seal the coverslip to the polycarbonate chamber. Therefore little or no silicone grease is needed and mechanical fasteners are not required for coverslip mounting. The chamber design consists of two parts; a polycarbonate chamber and a 40 mm diameter aluminum base.
The RC-43C closed bath chamber is supplied with one package each of #1.5 coverslips, 25 and 18 mm round, respectively, and spare O-rings. The distance between top and bottom coverslips is 1 mm. Additionally this chamber is also supplied with 10 feet of PE-160 and PE-90 polyethylene tubing.
The RC-43C closed bath design promotes an even solution exchange and eliminates changes in imaging focus due to alterations in bath height. Studies involving physiological bicarbonate buffers can be performed with no gas loss at the chamber interface.
Fluid is delivered to a 20 gauge stainless steel perfusion inlet port and removed from the chamber by means of a similar outlet port. The top coverslip is held in place with a retainer, a thin coating of silicone grease forms the seal between the chamber and the coverglass.
Series 40 chambers are designed to be compatible with Warner's QE-1 Quick Exchange Platform and DH-35iL and DH-40iL culture dish incubators which are compatible with Warner Series 20 microscope stage adapters.
A temperature controller TC-344C or TC-324C that connects to the heated platform QE-1 or the microincubators DH-35iL and DH-40iLcan be added to set and maintain a certain temperature.
Perfusion control can be achieved by adding a VCS Perfusion System, the DVW vacuum system takes care of the waste.
The RC-43C Chamber is supplied with one package each of #1.5 coverslips (25 and 18 mm round, respectively) and spare O-rings. Additionally, the RC-43C is supplied with 10 feet of PE-160 and PE-90 polyethylene tubing.
| Model | Bottom Coverslip |
Top Coverslip |
Aperture | Total Volume |
|---|---|---|---|---|
| RC-43C | 25 mm | 18 mm | 15.8 mm | 213 uL |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0349 | DH-35iL | Culture dish incubation system | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0102 | SH-27B | In-line solution heater | Login | Login |
| 64-0103 | SF-28 | Slow flow in-line solution heater | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-1492 | OR-2 | Replacement Retaining Ring o-ring for all RC-40 Series, 5 pack, black | Login | Login |
| 64-0375 | QE-1 | Quick change platform, heated base, for RC-40 and QR-40 series chambers | Login | Login |
| 64-1495 | OR-5 | Replacement o-ring for QR/RC-43C, 5 pack, red | Login | Login |
Related Products
-
(RC-40LP, RC-41LP, RC-42LP, RC-48LP) Low Profile Open Bath Chambers
-
(RC-40HP) High Profile Open Bath Chamber
-
(QR/RC-46SLP, QR/RC-47FSLP) Slotted Bath Chambers
-
(DH-35) Culture Dish Heater
-
(DH-35iL) Culture Dish Incubator
-
(DH-40iL) Culture Dish Microincubator
-
(QE-1) Quick Exchange Platform for 35-40 mm dishes
-
D263 Coverslip/Coverglass
Related Files
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0371 | RC-43C | Quick change chamber for 25 mm coverslips, closed bath | Login | Login |
| Glass Coverslips | ||||
| 64-0715 | CS-25R15 | Round cover glass, #1.5 thickness, 25 mm, 100 pack | Login | Login |
| 64-0714 | CS-18R15 | Round cover glass, #1.5 thickness, 18 mm, 100 pack | Login | Login |
| Accessories and Replacement Parts | ||||
| 64-1492 | OR-2 | Replacement Retaining Ring o-ring for all RC-40 Series, 5 pack, black | Login | Login |
| 64-1495 | OR-5 | Replacement o-ring for QR/RC-43C, 5 pack, red | Login | Login |
| Temperature Control | ||||
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0103 | SF-28 | Slow flow in-line solution heater | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0375 | QE-1 | Quick change platform, heated base, for RC-40 and QR-40 series chambers | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0349 | DH-35iL | Culture dish incubation system | Login | Login |
| 64-0102 | SH-27B | In-line solution heater | Login | Login |
| Perfusion and Waste Management | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |