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(RC-27NE2) Narrow Bath Chamber with Field Stimulation
Product Summary
This chamber has a narrower bath and smaller volume than the RC-27. It also has embedded electrodes to support studies using field stimulation.
- Designed for field stimulation studies
- Rectangular, open-bath chamber
- Narrow bath
- Accommodates tissue or brain slice specimens
- Applications include patch clamp and physiological measurements on cultured cells
The RC-27NE2 recording chamber is a modified version of the RC-27 with a narrower bath and smaller volume designed for applications requiring electric field stimulation. Laminar solution flow is more stable in this chamber than in the wider RC-27 chamber.
The working specimen area is 28 x 8 mm and there are inclined surfaces on both sides (from 2.0 mm to 3.1 mm height). The low profile design and inclined surfaces permits good electrode access to the specimen area.
The chamber is supplied with a pair of platinum wire electrodes installed along the parallel long sides of the bath. Electrode leads are approximately 16 cm long and terminate with 1 mm diameter pins. The RC-27NE2 is compatible with all available field stimulators.
The RC-27NE2 chamber requires a platform (PM-6 or PH-6) and stage adapter to complete the assembly for mounting onto a microscope. The chamber body is manufactured from polycarbonate and the chamber floor is formed from a 24 x 50 mm coverslip.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-24/50, 24 x 50 mm, pkg. of 50) and 10 ft of PE-160 polyethylene tubing.
| Material | Polycarbonate |
|---|---|
| Bath dimensions (L x W x H) |
37 x 8 x 2 mm |
| Outer dimensions (L x W x H) |
50 x 25 x 4 mm (with 8 mm boss to hold suction tube) |
| Volume, by depth | 305 μl/mm |
| Electrodes | Platinum wire 16 mm from chamber to 1 mm pins 23 mm length in bath |
| Minimum access angle (from side to center of bath) | 17° |
| Bottom coverglass | 24 x 50 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order. |
|---|---|---|---|---|
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
References
References
- Multiple pathological events in exercised dystrophic mdx mice are targeted by pentoxifylline: outcome of a large array of in vivo and ex vivo tests
Burdi R, Rolland JF, Fraysse B, Litvinova K, Cozzoli A, Giannuzzi V, Liantonio A, Camerino GM, Sblendorio V, Capogrosso RF, Palmieri B, Andreetta F, Confalonieri P, De Benedictis L, Montagnani M, De Luca A
J Appl Physiol. 2009 Apr;106(4):1311-24 - Gentamicin treatment in exercised mdx mice: Identification of dystrophin-sensitive pathways and evaluation of efficacy in work-loaded dystrophic muscle
De Luca A, Nico B, Rolland JF, Cozzoli A, Burdi R, Mangieri D, Giannuzzi V, Liantonio A, Cippone V, De Bellis M, Nicchia GP, Camerino GM, Frigeri A, Svelto M, Camerino DC.
Neurobiol Dis. 2008 Nov;32(2):243-53 - Fluvastatin and Atorvastatin Affect Calcium Homeostasis of Rat Skeletal Muscle Fibers in Vivo and in Vitro by Impairing the Sarcoplasmic Reticulum/Mitochondria Ca2+-Release System
Liantonio A, Giannuzzi V, Cippone V, Camerino GM, Pierno S, Camerino DC.
J Pharmacol Exp Ther. 2007 May;321(2):626-34 - Niflumic acid inhibits chloride conductance of rat skeletal muscle by directly inhibiting the CLC-1 channel and by increasing intracellular calcium
Liantonio A, Giannuzzi V, Picollo A, Babini E, Pusch M, Conte Camerino D
Br J Pharmacol. 2007 Jan;150(2):235-47 - Role of tumour necrosis factor [alpha], but not of cyclo-oxygenase-2-derived eicosanoids, on functional and morphological indices of dystrophic progression in mdx mice: a pharmacological approach
Pierno S, Nico B, Burdi R, Liantonio A, Didonna MP, Cippone V, Fraysse B, Rolland JF, Mangieri D, Andreetta F, Ferro P, Camerino C, Zallone A, Confalonieri P, De Luca A
Neuropathol Appl Neurobiol. 2007 Jun;33(3):344-59
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0240R2 | RC-27NE2 | Narrow Bath Recording Chamber w/ Field Stimulation | Login | Login |
| Platforms | ||||
| 64-1527 | PM-6 | PM-6 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0289 | PH-6 | PH-6 Platform for Series 20 chambers, heater | Login | Login |
| Replacement Consumables | ||||
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |