Designed for measuring drug, nutrient, water, and other transport across intact intestine
The base package is comprised of six tubular Ussing chambers, a chamber holder, a water bath, Tygon tubing for the perfusion lines, and an
assembly tool. Temperature in the water bath (tray) is maintained by a thermostatically controlled circulator such as our Grant TC-120.
An additional accessory needed to complete the system are peristaltic pumps (up to 12 channels) sufficient to drive solution through both the luminal and extraluminal spaces associated with each of the six intestinal sections.
This system has been or is currently in use at Ely Lilly and Yale University.
The Tubular Ussing System is designed to support up to six intestinal sections ranging in length from 2 to 8 cm within small plastic tubes that are then submerged in a thermostatically controlled waterbath. The intestinal sections are cannulated onto movable supports that allow the section to be positioned without stretch within the chamber tube without contacting the tube walls.
Perfusing solutions, usually with dissimilar composition, flow independently through the luminal and extraluminal sides of the intestine. This configuration allows samples to be collected from either side of the intestine over time for longitudinal transport measurements.
Intestine length1 to 4” (10 cm)
Intestine ODNo greater than ½” (1 cm)
Intestine IDNo less than 1/8” (3 mm)
Luminal perfusion rateUp to 8 ml/min (value subject to change)
SpeciesNo species restriction beyond size
-known to support mouse, rat, guinea pig
Ussing tube materialPolycarbonate
Ussing tube end capsDelrin (white)
Perfusion tube fittingsNylon, barbed
PerfusionLuminal and extraluminal flow maintained by peristaltic pump, 2 channels needed per chamber
Perfusion rateUp to 10 ml/min (value subject to change)
DimensionsRack assembly, 18 x 18 x 5 cm
Slots6, one for each chamber
SlotsSeparation 2.5 cm
Chamber water bath
Dimensions32.5 x 26.5 x 15 cm
Inlet and outlet portsNylon, 3/8" (9 mm) barb
Temperature range5-50°C, maintained by thermostatic water circulator
Customers from Europe, Australia, and Latin America can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
|Order No.||Model No.||Product Description||USA Price||Order|
|64-3056||GUT-6||Gastrointestinal Ussing Tube System, 6 channel, complete||Login||Login|
|72-4924||TC-120||Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz||Login||Login|
|72-4921||TC-120||Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz||Login||Login|
|64-3067||TY-38||Tygon tubing, 3/8" x 1/2" x 25 ft (ID x OD x length)||Login||Login|
|64-0167||TY-50||Tygon tubing, 1/16" x 1/8" x 50 ft (ID x OD x length)||Login||Login|
|64-3057||GT-1||Replacement tube with fixed and variable end caps||Login||Login|
|64-1504||HE-10||Handle extension, 7.9 mm dia x 12.6 cm long, 10-32 male-female thread||Login||Login|
|64-3058||GT-2||Replacement tube without end caps||Login||Login|
|64-3063||RVE||Replacement variable end cap||Login||Login|
|64-3062||RFE||Replacement fixed end cap||Login||Login|
|64-3060||GT-3||Replacement tube rack||Login||Login|
|64-3059||RW-1||Replacement water tank||Login||Login|
|64-3061||HT-12||Replacement heat exchanger block||Login||Login|