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Specialized Tools for Electrophysiology and Cell Biology Research

SmartUSSING Ussing Chamber System

Product Summary

SmartUSSING: An Ussing System That’s Built for Biologists

SmartUSSING is a better way to increase the impact of your Ussing experiments by adding electrophysiological data. With an easy-to-use design, user-friendly software, and a smaller footprint, SmartUSSING makes electrophysiology easy and accessible for anyone.

 

  • Automatically calibrate amplifiers in seconds
  • Modern, easy-to-use software
  • Space-saving design

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The easiest electrophysiology system you’ll ever use.

With SmartUSSING, setup is a breeze, and there’s zero learning curve. Each amplifier is automatically calibrated by the software, meaning that no manual calibrations are necessary. Just plug it in, and you’re ready to go. Training is fast, easy, and painless – even if you’ve never done electrophysiological experiments before.

SmartUSSING is a vertical, multi-chamber Ussing system that supports measurements from native tissue, including from the stomach, large or small intestine, gallbladder or urinary bladder, skin, trachea, nasal epithelia, cornea, and more. It’s also an excellent solution for research on both primary and secondary cell monolayers from primary sources, including renal tubes, the pancreas, salivary or sweat glands, IPSCs, and immortalized cell lines (including CACO-2 and MDCKr).

With an easy setup and user-friendly software, SmartUSSING dramatically reduces the possibility for errors, meaning more accurate data and more reliable results.

 

3 Ways SmartUSSING is different

 
  • Zero preparation of agar bridge electrodes. Your electrodes are ready to go.
  • DC-offset and fluid resistance compensation is adjusted automatically, with just one click. All values are saved in the software.
  • Up to 40% faster setup of new experiments, thanks to an easy setup process with fewer manual handling steps.
 

Maximum flexibility. Minimum stress.

SmartUSSING supports up to 6 chambers per holder, and up to 4 SmartUSSING systems can be connected to the same computer – giving you the flexibility to run up to 24 chambers in parallel. You can conduct up to 24 totally separate experiments at once, or maximize throughput by running up to 24 repetitions of the same experiment simultaneously. We offer flexible packages that allow you to purchase only the chambers you need.

 

USSING Control: Software designed by biologists, for biologists.

SmartUSSING drastically reduces the amount of manual configuration and overall technical knowledge required to set up your amplifier and create recordings within your software program, making it significantly faster and easier to launch your experiments and analyze data. Our software, USSING Control, works so well for biologists because it was designed by biologists. With fast processing and a user-friendly interface, USSING Control makes data collection easier than any other program you’ll ever use.

  • View data streams from up to 6 chambers at once
  • Set recording mode on a per-chamber basis, or apply the same settings to all chambers
  • Flexible, intuitive data visualizations that allow you to group leaders and followers
  • Fast, easy measurement and compensation of electrode offset and fluid resistance
  • Predefined protocols for all modes that allow you to set up your experiment in seconds – including voltage clamp, current clamp, ISC, and TEER (trans-epithelial electrical resistance)
  • Protocol editor allows you to create and save custom protocols for any mode, and specify pulse attributes and timing
  • View raw or analyzed data
 

A compact, low-noise design that fits in even the most crowded labs.

While traditional USSING systems require a separate set of amplifiers and AD converters, SmartUSSING utilizes powerful miniature amplifiers mounted directly to the back of the chamber holder – meaning a 50% reduction in footprint, as well as easier setup, shorter cables, and less noise in your recordings. It’s a win-win.

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Material Acrylic

For all chambers not using culture cups:

Increase the tissue dimensions by a minimum of 3.0 mm in each direction
to allow the o-ring or pins to capture the tissue without tearing.

For example, a chamber with a 5 mm round aperture will require a tissue with a minimum diameter of 8 mm,
and a chamber with a 2 x 10 mm oblong aperture will require a tissue with minimum dimension of 5 x 13 mm.


Suggested application(s) by aperture

Aperture Applications Aperture area Volume range
(per side)
4 mm round with pins general use 0.12 cm2 4-10 ml
9 mm round with pins general use 0.34 cm2 6-14 ml
4 x 8 mm oblong with pins rat or larger intestine
general use
0.29 cm2 2.5-10 ml
6 x 9 mm oblong with pins -  Discontinued rat and larger intestine
general use
0.46 cm2 4-12 ml
5 x 24 mm oblong with pins rat and larger intestine
general use
1.15 cm2  
8 x 24 mm oblong with pins rat or larger intestine
general use
1.78 cm2  
Snapwell cultured cells, cornea 1.12 cm2 8-14 ml
3 mm round, low volume, with pins biopsy
general use
0.07 cm2 1-3 ml
5 mm round, low volume, with pins general use 0.20 cm2 1.5-3 ml
9 mm round, low volume, with pins general use 0.64 cm2 2-4 ml
12 mm round, low volume, with pins general use 1.13 cm2 6-9 ml
2 x 10 mm oblong, low volume, with pins mouse intestine
general use
0.10 cm2  
4 x 8 mm oblong, low volume, with pins rat or larger intestine
general use
0.28 cm2 1.5-3 ml

System Assembly

NaviCyte Vertical Ussing System

Chamber openings

NaviCyte Vertical Ussing System

Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.

Order No. Model No. Product Description USA Price Order
64-3126 SmartUSSING Data Acquisition Interface (Rail) Login Login
64-3125 SmartUSSING Epithelial Clamp (Amplifier) Login Login
Base Assembly
66-0075 6-Chamber heater block assembly (includes heating block, air/gas manifold, clear front panel, retaining ring clamp tool) Login Login
Standard Volume Navicyte Vertical
66-0014 Standard Vertical Ussing/Diffusion Chamber, 9 mm diameter aperture, 0.64 cm2 area Login Login
66-0032 Standard Vertical Ussing/Diffusion Chamber, 4 x 8 mm aperture, 0.29 cm2 area Login Login
66-0046 Standard vertical Ussing/diffusion chamber, 5 x 24 mm aperture, 1.15 cm2 area Login Login
66-0013 Standard Vertical Ussing/Diffusion Chamber, 8 x 24 mm aperture, 1.78 cm2 area Login Login
Low Volume NaviCyte Vertical (includes electrode screws)
66-0040 Low volume vertical ussing/diffusion chamber, 3 mm diameter aperture, 0.07 cm2 area Login Login
66-0026 Low volume vertical ussing/diffusion chamber, 5 mm diameter aperture, 0.20 cm2 area Login Login
66-0015 Low volume vertical ussing/diffusion chamber, 9 mm diameter aperture, 0.64 cm2 area Login Login
66-0027 Low volume vertical ussing/diffusion chamber, 12 mm diameter aperture, 1.13 cm2 area Login Login
66-0034 Low volume vertical ussing/diffusion chamber, 2 x 10 mm aperture, 0.10 cm2 area Login Login
66-0042 Low volume vertical ussing/diffusion chamber, 4 x 8 mm aperture, 0.28 cm2 area Login Login
Specialty
66-0008 Specialty Vertical Ussing/Diffusion chamber, Snapwell, 1.13 cm2 Login Login
66-0020 Electrode Cap for Snapwell chambers, L/R pair Login Login
Chamber Caps
66-0018 Electrode Cap for standard volume chamber , L/R pair Login Login
66-0019 Perfusion Cap for standard volume chamber , L/R pair Login Login
66-0020 Electrode Cap for Snapwell chambers, L/R pair Login Login
Electrodes and Components
66-0023 Ag/AgCl electrodes with KCl, 100 cm leads, 4 pack (sufficient for 1 chamber) Login Login
66-0024 Glass barrel for Ag/AgCl electrode, with ceramic tip, 8 pack Login Login
66-0049 Open screws with o-rings for low volume chambers. Serves as electrode holders, 12 pack Login Login
Thermocirculator
72-4924 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz Login Login
72-4921 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz Login Login
64-2244 WB-KIT TC-120 Thermocirculator Tubing Kit Login Login

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