Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-21B, RC-21BR) Large Closed Bath Imaging Chambers
Product Summary
Polycarbonate chambers providing a large viewing area and good access for microscope objectives.
- Closed bath chamber design
- Diamond fluidics
- Ideal for fluorescent, calcium, and time-lapse imaging studies
- Uses 22 mm square or 25 mm round coverslips
- Large optical imaging area
- Good access for upright or inverted microscopes
The RC-21B and RC-21BR chambers feature a closed bath, short working distances, and linear solution flow. Distance between the top and bottom coverslip is 2.5 mm and total bath volume is 358 µl.
At the bath output, a length of tubing is used as a back pressure control to insure smooth solution flow. An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe. This port is useful for injecting substances or for removing air bubbles from the sealed chamber.
Studies involving physiological bicarbonate buffers can be performed with these chambers with no gas loss at the chamber interface. The closed design allows the use of permeable supports. With laminar flow, minimal distortion of the sample occurs during perfusion.
RC-21B and RC-21BR chambers require a platform (PM-2, PH-2 or P-2) and Series 20 stage adapter to complete the assembly for mounting onto a microscope.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22S or CS-25R, respectively, 100/pkg) and 10 ft of PE-160 polyethylene tubing.
| RC-21B | RC-21BR | |
|---|---|---|
| Material | polycarbonate | polycarbonate |
| Bath dimensions (L x W x H) |
17 x 13 x 2.5 mm | 17 x 13 x 2.5 mm |
| Outer dimensions (L x W x H) |
47.5mm x 28.5mm x 2.5mm | 47.5mm x 28.5mm x 2.5mm |
| Volume | 260 μl | 260 μl |
| Top coverglass | 22 mm square | 25 mm round |
| Bottom coverglass | 22 mm square | 25 mm round |
| Injection port (ID x OD) |
0.91 x 0.56 mm, stainless steel | 0.91 x 0.56 mm, stainless steel |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
| Output tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0704 | CS-22S | Square cover glass, #1 thickness, 22 x 22 mm, 100 pack | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
References
References
- Live Imaging of Dense-core Vesicles in Primary Cultured Hippocampal Neurons
Kwinter D, Michael S
J Vis Exp. 2009 May 29;(27). pii: 1144. doi: 10.3791/1144 - Monitoring the in vivo redox state of plant mitochondria: Effect of respiratory inhibitors, abiotic stress and assessment of recovery from oxidative challenge
Schwarzländer M, Fricker MD, Sweetlove LJ
Biochim Biophys Acta. 2009 May;1787(5):468-75. Epub 2009 Feb 3 - Influence of the Dissolution Rate on the Collapse and Shedding Behavior of Monostearin/Monopalmitin-rich Coated Microbubbles
Shen Y, Powell RL, Longo ML
Langmuir. 2008 Sep 16;24(18):10035-40. Epub 2008 Aug 22 - Confocal imaging of glutathione redox potential in living plant cells
Schwarzländer M, Fricker MD, Müller C, Marty L, Brach T, Novak J, Sweetlove LJ, Hell R, Meyer AJ
J Microsc. 2008 Aug;231(2):299-316 - Intracellular pH Homeostasis Plays a Role in the Tolerance of Debaryomyces hansenii and Candida zeylanoides to Acidified Nitrite
Mortensen HD, Jacobsen T, Koch AG, Arneborg N
Appl Environ Microbiol. 2008 Aug;74(15):4835-40. Epub 2008 Jun 6 - Characterizing molecular probes for diffusion measurements in the brain
Kaur G, Hrabetova S, Guilfoyle DN, Nicholson C, Hrabe J
J Neurosci Methods. 2008 Jun 30;171(2):218-25. Epub 2008 Mar 28 - Styryl Dye-Based Synaptic Vesicle Recycling Assay in Cultured Cerebellar Granule Neurons
Anggono V, Cousin MA, Robinson PJ
Methods Mol Biol. 2008;457:333-45 - A Fluorescent Sensor for Imaging Reversible Redox Cycles in Living Cells
Evan W. Miller, Shelly X. Bian, Christopher J. Chang
J Am Chem Soc, 2007 - pubs.acs.org - NHE1 inhibition by amiloride- and benzoylguanidine-type compounds. Inhibitor binding loci deduced from chimeras of NHE1 homologues with endogenous differences in inhibitor sensitivity
Pedersen SF, King SA, Nygaard EB, Rigor RR, Cala PM
J Biol Chem. 2007 Jul 6;282(27):19716-27. Epub 2007 May 10 - The Polypeptide Composition of Moving and Stationary Neurofilaments in Cultured Sympathetic Neurons
Yan Y, Jensen K, Brown A
Cell Motil Cytoskeleton. 2007 Apr;64(4):299-309 - D-box is required for the degradation of human Shugoshin and chromosome alignment
Fu G, Hua S, Ward T, Ding X, Yang Y, Guo Z, Yao X
Biochem Biophys Res Commun. 2007 Jun 8;357(3):672-8. Epub 2007 Apr 12 - Chemical inhibition of the Pho85 cyclin-dependent kinase reveals a role in the environmental stress response
Carroll AS, Bishop AC, DeRisi JL, Shokat KM, O'Shea EK
Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12578-83
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0224 | RC-21B | Large bath chamber, 260 µl volume, 22 mm square coverslip | Login | Login |
| 64-0225 | RC-21BR | Large bath chamber, 260 µl volume, 25 mm round coverslip | Login | Login |
| Platforms | ||||
| 64-1561 | PM-2 | PM-2 Platform for Series 20 chambers, magnetic clamps, heated | Login | Login |
| 64-0285 | PH-2 | PH-2 Platform for Series 20 chambers, heater | Login | Login |
| 64-0278 | P-2 | P-2 Platform for Series 20 chambers, non-heater | Login | Login |
| Replacement Consumables | ||||
| 64-0704 | CS-22S | Square cover glass, #1 thickness, 22 x 22 mm, 100 pack | Login | Login |
| 64-0705 | CS-25R | Round cover glass, #1 thickness, 25 mm, 100 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |