Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-22, RC-22C) Open Diamond Bath Imaging Chambers
Product Summary
The RC-22 and RC-22C are small volume, open bath recording chambers designed for tissue and brain slice studies.
- Designed for tissue slice samples or cell cultured coverslips
- Applications include patch clamp, intracellular / extracellular recordings, and imaging
- Diamond shaped bath provides laminar solution flow
- Small bath volume facilitates rapid solution exchange
- Tissue slice anchor included
The RC-22 and RC-22C feature an open bath and include a slice anchor. The diamond shape geometry of these chamber baths facilitate laminar solution flow which can provide minimal distortion of the sample during perfusion.
Slices are secured via a supplied stainless steel anchor (or harp). Threads are made of Lycra© and have a spacing of 1.5 mm. The RC-22 is supplied with an SHD-22L/15 anchor, and the RC-22C is supplied with an SHD-22CL/15 anchor. While anchor mass (typically 300 milligrams) may be sufficient to hold a slice in place, Warner anchors rely on a close fit between the anchor body and the chamber sidewall. This feature can reduce stress on the anchored slice.
Solution aspiration is via a stainless steel suction tube which is secured by a clamp. This insures that the adjustable-height suction tube will not move once set by the user. Each chamber has an agar bridge well for reference electrodes. The working volume of the RC-22 and RC-22C is 115 µl and 138 µl, respectively. Chamber sidewalls have a height of 1.2 mm to facilitate electrode access and placement.
The RC-22 and RC-22C chambers require a platform (PM-1, PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting onto a microscope. The bottom-forming coverslip is 22 x 40 mm.
Each chamber is supplied with a slice anchor, a swatch of nylon mesh, a pack of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.
| RC-22 | RC-22C | |
|---|---|---|
| Material | polycarbonate | polycarbonate |
| Bath dimension (L x W x H) |
22 x 8 x 3.8 mm | 23 x 10 x 3.8 mm |
| Volume, by depth | 115 μl/mm | 138 μl/mm |
| Minimum access angle (from side to center of bath) | 27° | 27° |
| Bottom coverglass | 22 x 40 mm | 22 x 40 mm |
| Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0246 | SHD-22L/15 | Slice anchor for RC-22, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0247 | SHD-22CL/15 | Slice anchor for RC-22C, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0198 | NYL/MESH | Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
References
Videos
- Perfusion and Aspiration Techniques
Warner Instruments
YouTube 2012
References
- Tuning the excitability of midbrain dopamine neurons by modulating the Ca2+ sensitivity of SK channels
Ji H, Hougaard C, Herrik KF, Strøbaek D, Christophersen P, Shepard PD
Eur J Neurosci. 2009 May;29(9):1883-95. Epub 2009 Apr 20 - Excitatory synapses in the rat superficial dorsal horn are strengthened following peripheral inflammation during early postnatal development
Li J, Baccei ML
Pain. 2009 May;143(1-2):56-64. Epub 2009 Feb 26 - Multiple pathological events in exercised dystrophic mdx mice are targeted by pentoxifylline: outcome of a large array of in vivo and ex vivo tests
Burdi R, Rolland JF, Fraysse B, Litvinova K, Cozzoli A, Giannuzzi V, Liantonio A, Camerino GM, Sblendorio V, Capogrosso RF, Palmieri B, Andreetta F, Confalonieri P, De Benedictis L, Montagnani M, De Luca A
J Appl Physiol. 2009 Apr;106(4):1311-24. Epub 2009 Jan 8 - Cell Electrophysiology with Carbon Nanopipettes
Schrlau MG, Dun NJ, Bau HH
ACS Nano. 2009 Mar 24;3(3):563-8 - Peripheral nerve injury sensitizes neonatal dorsal horn neurons to tumor necrosis factor
Li J, Xie W, Zhang JM, Baccei ML
Mol Pain. 2009 Mar 2;5:10 - The Role of L- and T-Type Calcium Channels in Local and Remote Calcium Responses in Rat Mesenteric Terminal Arterioles
Braunstein TH, Inoue R, Cribbs L, Oike M, Ito Y, Holstein-Rathlou NH, Jensen LJ
J Vasc Res. 2009;46(2):138-51. Epub 2008 Sep 2 - Decreased number of interneurons and increased seizures in neuropilin 2 deficient mice: Implications for autism and epilepsy
Gant JC, Thibault O, Blalock EM, Yang J, Bachstetter A, Kotick J, Schauwecker PE, Hauser KF, Smith GM, Mervis R, Li Y, Barnes GN
Epilepsia. 2009 Apr;50(4):629-45. Epub 2008 Jul 24 - Oxidative impairment of hippocampal long-term potentiation involves activation of protein phosphatase 2A and is prevented by ketone bodies
Maalouf M, Rho JM
J Neurosci Res. 2008 Nov 15;86(15):3322-30 - Gentamicin treatment in exercised mdx mice: Identification of dystrophin-sensitive pathways and evaluation of efficacy in work-loaded dystrophic muscle
De Luca A, Nico B, Rolland JF, Cozzoli A, Burdi R, Mangieri D, Giannuzzi V, Liantonio A, Cippone V, De Bellis M, Nicchia GP, Camerino GM, Frigeri A, Svelto M, Camerino DC
Neurobiol Dis. 2008 Nov;32(2):243-53. Epub 2008 Jul 23 - Developmental Changes in the Fidelity and Short-Term Plasticity of GABAergic Synapses in the Neonatal Rat Dorsal Horn
Ingram RA, Fitzgerald M, Baccei ML
J Neurophysiol. 2008 Jun;99(6):3144-50. Epub 2008 Apr 9 - Action potential throughput in aged rat hippocampal neurons: Regulation by selective forms of hyperpolarization
Gant JC, Thibault O
Neurobiol Aging. 2008 Mar 24 - Excitatory effects of human immunodeficiency virus 1 Tat on cultured rat cerebral cortical neurons
Brailoiu GC, Brailoiu E, Chang JK, Dun NJ
Neuroscience. 2008 Feb 6;151(3):701-10. Epub 2007 Dec 5 - Odorant receptors directly activate phospholipase C/inositol-1,4,5-trisphosphate coupled to calcium influx in Odora cells
Guang Liu, Robert M. Badeau, Akihiko Tanimura and Barbara R. Talamo
Journal of Neurochemistry, 2006, 96, 1591–1605 - Perinatal hyperoxia for 14 days increases nerve conduction time and the acute unitary response to hypoxia of rat carotid body chemoreceptors
David F. Donnelly, Insook Kim, Claire Carle and John L. Carroll
J Appl Physiol 99: 114-119, 2005
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0228 | RC-22 | Recording chamber for tissue slice, with SHD-22L/15 anchor | Login | Login |
| 64-0229 | RC-22C | Recording chamber for tissue slice, with SHD-22CL/15 anchor | Login | Login |
| Platforms | ||||
| 64-1526 | PM-1 | PM-1 Platform for Series 20 chambers, heated, magnetic clamps | Login | Login |
| 64-0284 | PH-1 | PH-1 Platform for Series 20 chambers, heater | Login | Login |
| 64-0277 | P-1 | P-1 Platform for Series 20 chambers, non-heater | Login | Login |
| Slice anchors (Harps) - RC-22 | ||||
| 64-0263 | SHD-22KIT | Slice anchor kit for RC-22, stainless steel | Login | Login |
| 64-0248 | SHD-22L/10 | Slice anchor for RC-22, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0246 | SHD-22L/15 | Slice anchor for RC-22, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0198 | NYL/MESH | Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk | Login | Login |
| 64-1412 | SHD-22F/10 | Slice anchor for RC-22, 1.0 mm thread spacing, flat frame, stainless steel | Login | Login |
| 64-1411 | SHD-22F/15 | Slice anchor for RC-22, 1.5 mm thread spacing, flat frame, stainless steel | Login | Login |
| Slice anchors (Harps) - RC-22C | ||||
| 64-0264 | SHD-22CKIT | Slice anchor kit for RC-22C, stainless steel | Login | Login |
| 64-0249 | SHD-22CL/10 | Slice anchor for RC-22C, 1.0 mm thread spacing, stainless steel | Login | Login |
| 64-0247 | SHD-22CL/15 | Slice anchor for RC-22C, 1.5 mm thread spacing, stainless steel | Login | Login |
| 64-0198 | NYL/MESH | Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk | Login | Login |
| 64-1414 | SHD-22CF/10 | Slice anchor for RC-22C, 1.0 mm thread spacing, flat frame, stainless steel | Login | Login |
| 64-1413 | SHD-22CF/15 | Slice anchor for RC-22C, 1.5 mm thread spacing, flat frame, stainless steel | Login | Login |
| Replacement Consumables | ||||
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| Replacement Parts | ||||
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
| 64-3018 | PM-plate | Pressure plates for PM Series platforms, 2 pack | Login | Login |
| 64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |