The RC-22 and RC-22C are small volume, open bath recording chambers designed for tissue and brain slice studies.
The RC-22 and RC-22C feature an open bath and include a slice anchor. The diamond shape geometry of these chamber baths facilitate laminar solution flow which can provide minimal distortion of the sample during perfusion.
Slices are secured via a supplied stainless steel anchor (or harp). Threads are made of Lycra© and have a spacing of 1.5 mm. The RC-22 is supplied with an SHD-22L/15 anchor, and the RC-22C is supplied with an SHD-22CL/15 anchor. While anchor mass (typically 300 milligrams) may be sufficient to hold a slice in place, Warner anchors rely on a close fit between the anchor body and the chamber sidewall. This feature can reduce stress on the anchored slice.
Solution aspiration is via a stainless steel suction tube which is secured by a clamp. This insures that the adjustable-height suction tube will not move once set by the user. Each chamber has an agar bridge well for reference electrodes. The working volume of the RC-22 and RC-22C is 115 µl and 138 µl, respectively. Chamber sidewalls have a height of 1.2 mm to facilitate electrode access and placement.
The RC-22 and RC-22C chambers require a platform (PM-1, PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting onto a microscope. The bottom-forming coverslip is 22 x 40 mm.
Each chamber is supplied with a slice anchor, a swatch of nylon mesh, a pack of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.
RC-22 | RC-22C | |
---|---|---|
Material | polycarbonate | polycarbonate |
Bath dimension (L x W x H) |
22 x 8 x 3.8 mm | 23 x 10 x 3.8 mm |
Volume, by depth | 115 μl/mm | 138 μl/mm |
Bottom coverglass | 22 x 40 mm | 22 x 40 mm |
Input tubing (ID x OD) |
1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
Aspirator coupling | 1.67 mm (OD) | 1.67 mm (OD) |
Order No. | Model No. | Product Description | USA Price | Order |
---|---|---|---|---|
64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
64-0246 | SHD-22L/15 | Slice anchor for RC-22, 1.5 mm thread spacing, stainless steel | Login | Login |
64-0247 | SHD-22CL/15 | Slice anchor for RC-22C, 1.5 mm thread spacing, stainless steel | Login | Login |
64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
64-0198 | NYL/MESH | Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk | Login | Login |
64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
Videos
References
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
Order No. | Model No. | Product Description | USA Price | Order |
---|---|---|---|---|
Chambers | ||||
64-0228 | RC-22 | Recording chamber for tissue slice, with SHD-22L/15 anchor | Login | Login |
64-0229 | RC-22C | Recording chamber for tissue slice, with SHD-22CL/15 anchor | Login | Login |
Platforms | ||||
64-1526 | PM-1 | PM-1 Platform for Series 20 chambers, heated, magnetic clamps | Login | Login |
64-0284 | PH-1 | PH-1 Platform for Series 20 chambers, heater | Login | Login |
64-0277 | P-1 | P-1 Platform for Series 20 chambers, non-heater | Login | Login |
Slice anchors (Harps) - RC-22 | ||||
64-0263 | SHD-22KIT | Slice anchor kit for RC-22, stainless steel | Login | Login |
64-0248 | SHD-22L/10 | Slice anchor for RC-22, 1.0 mm thread spacing, stainless steel | Login | Login |
64-0246 | SHD-22L/15 | Slice anchor for RC-22, 1.5 mm thread spacing, stainless steel | Login | Login |
64-0198 | NYL/MESH | Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk | Login | Login |
64-1412 | SHD-22F/10 | Slice anchor for RC-22, 1.0 mm thread spacing, flat frame, stainless steel | Login | Login |
64-1411 | SHD-22F/15 | Slice anchor for RC-22, 1.5 mm thread spacing, flat frame, stainless steel | Login | Login |
Slice anchors (Harps) - RC-22C | ||||
64-0264 | SHD-22CKIT | Slice anchor kit for RC-22C, stainless steel | Login | Login |
64-0249 | SHD-22CL/10 | Slice anchor for RC-22C, 1.0 mm thread spacing, stainless steel | Login | Login |
64-0247 | SHD-22CL/15 | Slice anchor for RC-22C, 1.5 mm thread spacing, stainless steel | Login | Login |
64-0198 | NYL/MESH | Nylon mesh kit, very fine mesh, 7x7 cm, 10 pk | Login | Login |
64-1414 | SHD-22CF/10 | Slice anchor for RC-22C, 1.0 mm thread spacing, flat frame, stainless steel | Login | Login |
64-1413 | SHD-22CF/15 | Slice anchor for RC-22C, 1.5 mm thread spacing, flat frame, stainless steel | Login | Login |
Replacement Consumables | ||||
64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
64-0709 | CS-24/50 | Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack | Login | Login |
64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
Temperature Control | ||||
64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
Accessories | ||||
64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
Replacement Parts | ||||
64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
64-3018 | PM-plate | Pressure plates for PM Series platforms, 2 pack | Login | Login |
64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |