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(RC-24, RC-24E) Fast Exchange Diamond Bath Chambers
Product Summary
The RC-24 and RC-24E chambers allow for very fast solution exchange for a variety of applications. These recording chambers feature the smallest bath volumes of Warner's open style chambers.
- Designed for physiological measurements of cell cultured coverslips
- Applications include patch clamp, imaging, and intracellular/extracellular recordings
- Diamond shaped bath provides laminar solution flow
- Both perfusion and aspiration ports on same side of chamber
- Very small bath volume facilitates very fast solution exchange
The bath volume in these models is among the smallest of the open style chambers. This enables very fast solution exchange for a variety of applications, including patch clamp and electrophysiology studies. The small bath volumes are especially useful when using drugs in small quantities.
The RC-24 and RC-24E have a an unusual characteristic in that both the perfusion inlet and aspiration outlet is on the same side of the chamber. This allows the associated perfusion lines to be conveniently placed on only one side and facilitates electrode placement.
An agar bridge well for reference electrodes is provided, as is an aspiration well. The aspiration well is equipped with a stainless steel aspiration tube together with a clamp. The clamp insures that the adjustable suction tube will not move once the height is set by the user.
The floor of these chambers can be formed from either a 22 mm square coverslip or from a 22 mm x 40 mm rectangular coverslip. Each chamber requires a platform (PM-1, PH-1, P-1) and a Series 20 stage adapter to complete the assembly for mounting on a microscope.
Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22x40 mm, 50/pkg) and 10 ft of PE-160 tubing.
| RC-24 | RC-24E | |
|---|---|---|
| Material | Polycarbonate | Polycarbonate |
| Bath dimension (L x W x H) |
15 x 5 x 3.8 mm | 15 x 6 x 3.8 mm |
| Volume, by depth | 40 μl/mm | 50 μl/mm |
| Bottom coverglass | 22 x 40 mm | 22 x 40 mm |
| Input tubing (ID x OD) | 1.14 x 1.57 mm (PE-160) | 1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-0704 | CS-22S | Square cover glass, #1 thickness, 22 x 22 mm, 100 pack | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
References
References
- The Effect of Thermal Acclimation on Action Potentials and Sarcolemmal K+ Channels from Pacific Bluefin Tuna Cardiomyocytes
Galli GL, Lipnick MS, Block BA
Am J Physiol Regul Integr Comp Physiol. 2009 Aug;297(2):R502-9. Epub 2009 Jun 10 - Analogs of MK-499 are differentially affected by a mutation in the S6 domain of the hERG K+ channel
Karczewski J, Wang J, Kane SA, Kiss L, Koblan KS, Culberson JC, Spencer RH
Biochem Pharmacol. 2009 May 15;77(10):1602-11. Epub 2009 Feb 27 - Pharmacological properties of a pore induced by raising intracellular Ca2+
Faria RX, Reis RA, Casabulho CM, Alberto AV, de Farias FP, Henriques-Pons A, Alves LA
Am J Physiol Cell Physiol. 2009 Jul;297(1):C28-42. Epub 2009 Mar 25 - D1 dopaminergic control of G protein–dependent inward rectifier K+ (GIRK)–like channel current in pyramidal neurons of the medial prefrontal cortex
Witkowski G, Szulczyk B, Rola R, Szulczyk P
Neuroscience. 2008 Jul 31;155(1):53-63. Epub 2008 Jun 19 - Modulation of Ca2+ channel current by mu opioid receptors in prefrontal cortex pyramidal neurons in rats
Rola R, Jarkiewicz M, Szulczyk P
Acta Neurobiol Exp (Wars). 2008;68(1):10-25 - High [Na+]i in cardiomyocytes from rainbow trout
Birkedal R, Shiels HA
Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R861-6. Epub 2007 May 16 - Decreased Neuronal Death in Na+/H+ Exchanger Isoform 1-Null Mice after In Vitro and In Vivo Ischemia
Jing Luo, Hai Chen, Douglas B. Kintner, Gary E. Shull, and Dandan Sun
The Journal of Neuroscience, December 7, 2005, 25(49):11256-11268 - Orexin-A-induced Ca2+ Entry EVIDENCE FOR INVOLVEMENT OF TRPC CHANNELS AND PROTEIN KINASE C REGULATION*,
Kim P. Larsson, Hanna M. Peltonen, Genevieve Bart, Lauri M. Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P. Kukkonen, and Karl E. O. Åkerman,
J. Biol. Chem., Vol. 280, Issue 3, 1771-1781, January 21, 2005 - Stimulation of astrocyte Na+/H+ exchange activity in response to in vitro ischemia depends in part on activation of ERK1/2
Douglas B. Kintner, Andy Look, Gary E. Shull, and Dandan Sun
Am J Physiol Cell Physiol 289: C934-C945, 2005 - The contribution of ionic currents to changes in refractoriness of human atrial myocytes associated with chronic atrial fibrillation
Workman AJ, Kane KA, Rankin AC
Cardiovasc Res. 2001 Nov;52(2):226-35
Related Files
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| Chambers | ||||
| 64-0230 | RC-24 | Fast exchange recording chamber, 40 µl volume | Login | Login |
| 64-0231 | RC-24E | Fast exchange recording chamber, 50 µl volume | Login | Login |
| Platforms | ||||
| 64-1526 | PM-1 | PM-1 Platform for Series 20 chambers, heated, magnetic clamps | Login | Login |
| 64-0284 | PH-1 | PH-1 Platform for Series 20 chambers, heater | Login | Login |
| 64-0277 | P-1 | P-1 Platform for Series 20 chambers, non-heater | Login | Login |
| Replacement Consumables | ||||
| 64-0704 | CS-22S | Square cover glass, #1 thickness, 22 x 22 mm, 100 pack | Login | Login |
| 64-0707 | CS-22/40 | Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack | Login | Login |
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0756 | PE-160/100 | Polyethylene tubing, PE-160, 100 ft | Login | Login |
| Temperature Control | ||||
| 64-2401 | TC-344C | Heater controller, dual channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-2400 | TC-324C | Heater controller, single channel, 110-230 VAC, 50/60Hz | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Replacement Parts | ||||
| 64-1431 | ST-1S | Suction Tube, Series 20 Classic Design, straight style | Login | Login |
| 64-3018 | PM-plate | Pressure plates for PM Series platforms, 2 pack | Login | Login |
| 64-1506 | RPC-1 | Replacement platform clamps and screws for P and PH Series platforms, 2 pack | Login | Login |
| 64-0274 | RH-2 | Heater elements for Warner chambers and platforms, 2 pack | Login | Login |
| Accessories | ||||
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| 64-1920 | SA-20PS1 | Adapter Ring for Pecon Heating Insert P | Login | Login |