The Model 782 dual-channel dissolved oxygen monitoring system acts as both a stand alone oxygen meter, displaying and storing oxygen values, and also acts as an interface for logging respirometry data to a PC.
The 782 will display its readings in either pO2 units (e.g. mm Hg, torr, kPa) or any standard oxygen concentration units (e.g. µmol/l, µg/l, mg/l, µl/ml, ml/l, ppm,% saturation). It requires at least one ultraprecise 1302 Oxygen Sensor.
When used in conjunction with the supplied software, the 782 can be used either for closed cell respirometry (e.g., MT-200 mitocell or the RC-300 or RC-350 respiration cells) or for flow cell respirometry (e.g., FC-100 flowcell or MC-100 microcell). Alternatively the software can be used to monitor dissolved oxygen in isolated organ preparations.
The software package includes experiment set-up; data recording; and data analysis. When the software is used for monitoring dissolved oxygen, oxygen values at flagged or set points can also be directly transferred to the Results and Reports page.
The program has been written so that it is user-friendly and quick to learn. It can be used for closed cell respirometry (using the MT-200, RC-300, RC-350 or RC-650), flow respirometry (using the FC-100 or MC-100), or for monitoring dissolved oxygen (using any of the above). When used for respirometry experiments, it follows the familiar logical sequences of operations, from electrode calibration to recording and analysis.
The setup screen is used for entering all of the details which the computer needs in order to record in the correct units, and to express the respiration rate, normalised to biomass units if required, in the desired units. All experimental details entered here are transferred through to the final calculated results report page. During setup, the cell volumes (for closed cell) and flow rate, electrode positioning (for flow respirometry), temperature of the experiment, scrolling speed of the traces during recording, and the instruction to print the recording screen to printer (if hard copy is required) are all entered. Many of these will not change from one experiment to the next, and it is only necessary to enter changes.
Clicking the 'Start' button initiates the recording process, and traces the data scroll across the screen. If substrates, inhibitors or other solutions require to be added during the run, the position where this occurs can be recorded with a flag, and the nature of the addition is recorded on a drop down menu. At the termination of the experiment, the data are saved to a data file, and may be analyzed straight away if required.
The data file and recorded traces, and event marker flags are recalled to the screen. If the recording has exceeded one screen width, it can be contracted to fit the screen, if required. Alternatively it is possible to scroll along long recording traces. Selector lines may be dragged to enclose specific parts of the trace. Thus if the traces show both control respiration and changed respiration after adding a solution, the selector lines would be dragged to the control rate first and then to the changed rates.
In each case respiration rates (normalized if so designated) are calculated automatically by clicking the 'Calculate' button. The calculated rates are tabulated, together with the experimental details entered during Setup, on the report page. The results may be exported to a spreadsheet, word processing or statistics package. The Analysis screen and the Report page may be printed, if required.
When the software is used for dissolved oxygen monitoring, the Analysis screen displays the recorded traces and the oxygen values at flagged points or at set times can be transferred to the Results and report page. The program requires a computer with a minimum specification of: Pentium processor, 1 free serial port, 128 Mb RAM, Windows 95 or later and 4 MB hard disk space. A comprehensive Instruction Manual is also supplied.
The 782 is a dual-channel meter which can also measure dissolved oxygen. Includes an interface to PC respirometry software.
Uses up to two ultraprecise 1302 Oxygen Sensors
Displays any standard unit of pO2 or oxygen concentration
Closed-cell and flow-through respirometry and oxygen monitoring applications available
System includes Model 782 meter, USB interface cable, and respirometry software
Resolution with 1302 electrode
0.1% of full scale
Off-line data storage
4 hours at 1/sec reading
Windows PC, USB port, with Windows 7 64 bit or earlier; 4 MB of hard disk space and at least 64 MB of RAM
5 VDC; 1 A, w/external plug
100-230 VAC, 47-63 Hz
185 x 135 x 105 mm (7.25 x 5.25 x 4.125 in)
Microcathode oxygen electrode
Mitocell miniature respirometer, 50-100 ul
Respiration cell, 0.31.0 ml
Respiration cell, 1.03.0 ml
Order No. (Model No.)
Dual channel oxygen meter with automatic respirometry software