In-line solution heating is the simplest and most effective method of warming perfusion solutions. The heater is connected to the chamber with a short length of tubing such that the warmed perfusate flows directly into the chamber bath. Depending on bath volume and other factors, in-line solution heating alone by it self may be sufficient for many applications.
In-line solution heaters from Warner Instruments are used to warm perfusate solutions as they flow into a chamber (such as our Imaging and Recording Chambers). Their small size allows for placement close to the chamber input allowing the perfusate to be warmed immediately prior to entry. The SH-27B is our rapid flow in-line solution heater. It is ideal for perfusion flows up to 10 mL/min while the SF-28 is recommended for applications requiring solution flow rates of less than 2 mL/min.
Temperature control is maintained by an internal thermistor (i.e. T1 on the heater controller) which senses the temperature at the outflow end of the heater. This sensing thermistor is located outside of the solution path and is therefore not subjected to corrosive salts or careless cleaning.
Each heater is also supplied with a Model TA-29 thermistor cable assembly (as shown on the right) which allows for monitoring the actual bath temperature (T2 output on the heater controller). Solutions are gradually warmed as they flow from input to output resulting in minimal outgassing. Heater input and output lines accept standard 1/16" OD (PE-160) tubing.
In-line solution heaters are the most direct way to warm solutions flowing into a Series 20 Imaging and Recording Chamber.
Small size allows placement close to imaging and recording recording so as to minimize convective heat losses
Single line in-line solution heaters include T1 thermister embedded within aluminum housing for feedback control