Polycarbonate chambers providing a large viewing area and good access for microscope objectives.
Closed bath chamber design
Ideal for fluorescent, calcium, and time-lapse imaging studies
Uses 22 mm square or 25 mm round coverslips
Large optical imaging area
Good access for upright or inverted microscopes
The RC-21B and RC-21BR chambers feature a closed bath, short working distances, and linear solution flow. Distance between the top and bottom coverslip is 2.5 mm and total bath volume is 358 µl.
At the bath output, a length of tubing is used as a back pressure control to insure smooth solution flow. An additional stainless steel port at the bath input fits a standard Hamilton microliter syringe. This port is useful for injecting substances or for removing air bubbles from the sealed chamber.
Studies involving physiological bicarbonate buffers can be performed with these chambers with no gas loss at the chamber interface. The closed design allows the use of permeable supports. With laminar flow, minimal distortion of the sample occurs during perfusion.