The MT-200 Mitocell Miniature Respirometer can be used in any situation where the sample size is limited. The base section contains an integral, solid state, fixed speed magnetic stirrer. The rear of the unit has attachments for connecting the MT-200 to a circulating source of constant temperature control. A 1302 Oxygen Sensor is inserted from below so that its protruding polypropylene or FEP membrane forms the floor of the respiration chamber. The MT-200 can be used in conjunction with either the 782 Dual-Channel Oxygen Meter or the 928 Six-Channel Oxygen Meter.
The glass chamber unit is comprised of an outer water jacket and a central, precision bore tube. The chamber floor is formed by the insertion of the polycarbonate plunger which can be inserted to various depths depending on the desired respiration chamber volume. Chamber volume calibration is achieved by means of a gauge piece, which is supplied.
Substrate or inhibitor can be directly added during the course of a respirometry run via the capillary bore in the plunger. A 1 µl syringe is supplied for the MT-200 and a 5 µl syringe is supplied with the larger volume MT-200A.
The chamber unit is sealed against the base by a threaded acetal collar. By unscrewing this collar the chamber unit can be removed for cleaning.
This microminiature respirometer is designed for respiration measurements of mitochondria isolated from biopsy samples.
Sample volumes from 50 µl to 1 ml
Glass chamber provides good visibility of contents
Transparent polycarbonate plunger
Allows direct injection of substrates and inhibitors