The PLI-100A picoinjector reliably delivers injections from femtoliters to nanoliters through micropipettes by applying a regulated pressure for a digitally set period of time.
Femtoliter to microliter injections
Digital readouts for injection count, time, and pressure
Reliable optically encoded circuit for setting injection time
5 pressures: inject, balance, clear, fill, and hold
Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-100A is up to the task.
Pressure to the pipette is controlled precisely through a multi-turn regulator and is reported digitally for reproducibility. Injection time is set using an optically encoded circuit that permits both fine and coarse settings from a single control knob. System timing can be controlled in three ways: push button, foot switch, or external trigger.
Unique Pressure Capabilities
The PLI-100A features three positive and two negative pressures.
The three positive functions allow the user to:
Inject - Inject picoliter to nanoliter amounts of fluids
Balance - Apply a balance pressure to prevent backflow into the micropipette following an injection
Clear - Clear a micropipette of material that is blocking the tip
The two negative (or vacuum) functions allow the user to:
Fill - Fill micropipettes from their tips
Hold - Provide a means to secure and manipulate a cell using holding pipette.
The PLI-100A's Balance, Hold, and Clear functions are features not found on other microinjection units.
In addition to the injection pressure, the PLI-100A offers a secondary balance pressure. This adjustable control applies a positive pressure to the injection pipette both before and after an injection that eliminates dilution caused by capillary action and aids in the prevention of clogging. Wasteful continuous injections that can occur when no separate balance pressure is available is avoided.
The PLI-100A has two separate vacuum generators used to fill a micropipette and to hold suspended cells while injecting. Filling of the pipette from the tip is less complicated than back-filling. The PLI-100A features a Fill Vacuum adjustment on the front panel.
Suspended cells can be held in place using a second (holding) pipette. The holding vacuum range accommodates most cell types.
A high-pressure pulse can be used for clearing a pipette should it happen to clog. This is especially useful when working with pipettes sized for smaller volumes.
BNC type connectors are available at the front panel to ease integrating the PLI with other equipment. Synchronization with or control from injections with a data acquisition system is possible.
The PLI-100A is available in three packages: Basic, Plus, and Deluxe:
The Basic system comes with with an input hose, output hose, holding hose and input hose adapter. The Plus system comes with everything in the Basic system with the addition of one foot switch and a stainless steel pipette holder. The Deluxe system comes with everything in the Plus system with the addition of a second foot switch and a second stainless steel pipette holder with input hose.
PLI-100A Deluxe Picoinjector with injection, balance, clear, filling and holding pressures, comes with input hose, output hose, holding hose, two foot switches, two pipette holders, and input hose adapter