Specialized Tools for Electrophysiology and Cell Biology Research

(RC-10) Round Bath Oocyte Recording Chamber

The RC-10 open bath chamber is suitable for physiological experiments performed with inverted or dissecting microscopes.

  • Patch clamp
  • Recording from Oocytes or isolated perfused tubules
  • Experiments involving cell impalement 

RC-10 Chamber

These RC-10 features an open bath design suitable for physiological experiments performed with inverted or dissecting microscopes, including patch and oocyte recording, isolated perfused tubule, and cell impalement studies. This chamber has also been used with fluorescence microscopy.

Made from polycarbonate, a user attached, #2 thickness coverslip (24 x 60 mm, ) becomes the floor of the bath. The coverslip can be attached with vacuum grease, or more permanently with Sylgard or RTV.

Solution can be introduced via gravity-feed and removed via suction, or alternatively, input and output can be pump-controlled. Flow rates can exceed 10 ml/min with gravity feed. The perfusion input accepts standard 1.57 mm OD polyethylene tubing (PE-160).

Solutions are removed from a separate suction reservoir to minimize noise in the recording chamber and the suction tube height is adjustable to control the bath solution level.

Multiple perfusion lines can be connected using an MP Series manifold in place of the single line connector supplied with the chamber. An isolated reservoir is provided for an agar bridge in the recording ground circuit.

The RC-10 has a 10 mm diameter bath and is recommended where small volumes and fast solution exchange is important. The bath volume is 160 μl at 2 mm solution height. A single agar bridge well is supplied.

Mounting the Chamber

The RC-10 is mounted onto a microscope with one of the RC Series Stage Adapters which are purchased separately.

Chambers are supplied with a single-input perfusion connector, a suction tube, and one package of #2 Coverslips (24 x 60 mm).

*Note: The double thickness of glass (bottom + sample coverslips) may not be compatible with some short working distance microscope objectives

Order No. Model No. Product Description USA Price Order
64-0107 TA-29 Cable with bead thermistor Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login

References

References

  1. Effect of psoralen on the cloned Kv3.1 currents
    Min Ji Sung, Sang June Hahn and Bok Hee Choi
    Arch Pharm Res. 2009 Mar;32(3):407-12. Epub 2009 Apr 23
  2. Open Channel Block of Kv3.1 Currents by Fluoxetine
    Sung MJ, Ahn HS, Hahn SJ, Choi BH
    J Pharmacol Sci. 2008 Jan;106(1):38-45. Epub 2008 Jan 11
  3. Apparent receptor-mediated activation of Ca2+-dependent conductive Cl– transport by shark-derived polyaminosterols
    Chernova MN, Vandorpe DH, Clark JS, Williams JI, Zasloff MA, Jiang L, Alper SL
    Am J Physiol Regul Integr Comp Physiol. 2005 Dec;289(6):R1644-58. Epub 2005 Aug 18
  4. Effects of osmotic shrinkage on voltage-gated Ca2 channel currents in rat anterior pituitary cells
    Ben-Tabou De-Leon S, Ben-Zeev G, Nussinovitch I
    Am J Physiol Cell Physiol. 2006 Jan;290(1):C222-32. Epub 2005 Sep 7
  5. CFTR fails to inhibit the epithelial sodium cannel ENaC expressed in Xenopus laevis oocytes
    Nagel G, Barbry P, Chabot H, Brochiero E, Hartung K, Grygorczyk R
    J Physiol. 2005 May 1;564(Pt 3):671-82. Epub 2005 Mar 3
  6. Acetylcholine Induces Hyperpolarization Mediated by Activation of K(Ca) Channels in Cultured Chick Myoblasts
    Doyun Lee, Jaehee Han, and Jae-Yong Park
    Korean J Physiol Pharmacol Vol 9: 37 43, February, 2005
  7. Redox signaling in colonial hydroids: many pathways for peroxide
    Blackstone NW, Bivins MJ, Cherry KS, Fletcher RE, Geddes GC
    J Exp Biol. 2005 Jan;208(Pt 2):383-90
  8. Functional Comparison of Mouse slc26a6 Anion Exchanger with Human SLC26A6 Polypeptide Variants DIFFERENCES IN ANION SELECTIVITY, REGULATION, AND ELECTROGENICITY
    Chernova MN, Jiang L, Friedman DJ, Darman RB, Lohi H, Kere J, Vandorpe DH, Alper SL
    J Biol Chem. 2005 Mar 4;280(9):8564-80. Epub 2004 Nov 17
Order No. Model No. Product Description USA Price Order
64-0306 RC-10 RC-10 open bath chamber with 10 mm bath Login Login
Accessories and Replacement Parts
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login

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