Specialized Tools for Electrophysiology and Cell Biology Research

Self-Contained Ussing Systems

Product Summary

The U9926 and U2500 Self-Contained Ussing chambers improve on the Classic design by bringing the solution reservoir into the chamber housing reducing the apparatus size and increasing ease-of-use

  • Modular design with separate inserts
  • Supports membranes from 3.8 to 13.5 mm wide
  • No glass circulation reservoirs
  • Supports Snapwell, Transwell, and Millicell culture cups
  • Chamber heating is provided by integral water jackets
  • Compatible with UssingChart hardware and software

The Self-Contained Ussing Chambers systems come in single- and dual-channel models and offer many advantages over the Classic design. The single-channel U9926 and dual-channel U2500 are shown above and to the right. Both systems make use of inserts to secure and position the tissue or culture cup within the chamber body.

Chamber bodies (single- and dual-chamber models) consist of chamber-halves which clamp around the insert(s). Each chamber-half has four ports that enter into its respective bath compartment: a voltage electrode port, a current electrode port, an air/gas port, and a solution drain port. Solution warming is provided by pumping heated water through each chamber-half and there are no delicate glass heat exchangers to be broken. The dual-chamber U2500 has the additional feature of including four needle valves for adjusting the air/gas mixture into each individual chamber-half.

 

Inserts can be easily exchanged between experiments. Solutions are removed from the chambers through the drain valves and the halves unclamped from each other. The insert is then simply replaced. Inserts are available to support a variety of tissue sizes and culture cups.

The U9926 (single-channel chamber is supplied with 1 electrode set (consisting of 2 voltage and 2 current electrodes), 12 bridge fittings with adapters; and a tubing/ fitting kit. The U2500 (dual channel) chamber is supplied with two each of the above described accessories. Chamber inserts are ordered separately.

Data Acquisition

The Self-Contained Ussing systems are compatible with electrophysiological epithelial transport measurements and use UssingChart data acquisition. This package includes an amplifier, a digitizer, software, and cables. You can view details on the UssingChart Data Acquisition webpage.

Self-Contained Ussing System Expandability

The self-contained Ussing system can be scaled by adding chambers and expanding the UssingChart DAQ.
Refer to the chart below for needed components:

Expansion Needed items Order No. Quantity to add
2 to 4 channels - order all items
  Dual-channel Ussing chamber base assembly
does not include inserts (order 2 separately)
64-0575S 1
  EC-825A dual channel epithelial voltage clamp 64-1605 1
2 to 6 channels - order all items
  Dual-channel Ussing chamber base assembly
does not include inserts (order 4 separately)
64-0575S 2
  EC-825A dual channel epithelial voltage clamp 64-1605 2
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
2 to 8 channels - order all items
  Dual-channel Ussing chamber base assembly
does not include inserts (order 6 separately)
64-0575S 3
  EC-825A dual channel epithelial voltage clamp 64-1605 3
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
4 to 6 channels - order all items
  Dual-channel Ussing chamber base assembly
does not include inserts (order 2 separately)
64-0575S 1
  EC-825A dual channel epithelial voltage clamp 64-1605 1
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
4 to 8 channels - order all items
  Dual-channel Ussing chamber base assembly
does not include inserts (order 4 separately)
64-0575S 2
  EC-825A dual channel epithelial voltage clamp 64-1605 2
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
6 to 8 channels - order all items
  Dual-channel Ussing chamber base assembly
does not include inserts (order 2 separately)
64-0575S 1
  EC-825A dual channel epithelial voltage clamp 64-1605 1

Working volume is 5-8 ml depending on insert.

640504_U9504,U9506,U9509,in

For all chambers not using culture cups:

Increase the tissue dimensions by a minimum of 3.0 mm in each direction
to allow the o-ring or pins to capture the tissue without tearing.

For example, a chamber with a 5 mm round aperture will require a tissue with a minimum diameter of 8 mm,
and a chamber with a 2 x 10 mm oblong aperture will require a tissue with minimum dimension of 5 x 13 mm.

Suggested application(s) by aperture

Aperture Applications Aperture area
3.8 mm round with o-ring biopsy
soft tissue
0.11 cm2
6 mm round with o-ring skin and soft tissue
general use
0.28 cm2
9 mm round with o-ring skin and soft tissue
general use
0.64 cm2
12 mm round with o-ring skin and soft tissue
cornea
general use
1.13 cm2
13.5 mm round with o-ring skin
cornea
general use
1.43 cm2
3.8 mm round with pins general use 0.11 cm2
6 mm round with pins general use 0.28 cm2
9 mm round with pins general use 0.64 cm2
12 mm round with pins skin
general use
1.13 cm2
13.5 mm round with pins skin
general use
1.43 cm2
1.6 x 7 mm oblong with pins mouse intestine
general use
0.11 cm2
2.4 x 7 mm oblong with pins mouse intestine
general use
0.17 cm2
3.5 x 7 mm oblong with pins mouse intestine
rat intestine
general use
0.25 cm2
Snapwell, 12.7 mm tissue culture 1.12 cm2
Millicell, 12 mm tissue culture 1.13 cm2
Transwell, 6.5 mm tissue culture 0.33 cm2
Order No. Model No. Product Description USA Price Order
64-0565 U9406 Tubing kit (hose and Luer fittings) Login Login
64-0554 U9975A Electrode set, includes 2 Ag/AgCl pellets and 2 Ag wires Login Login
64-1521 U9565SC Electrode bridge fitting kit (pipette tips and adapters), 12 pack Login Login
64-0555 U9985 Bridge fitting adapters, 6 pack Login Login
64-0604 U9605 O-rings for U9926 and U2500 Ussing chambers, 4 pack Login Login

References

  1. Extracellular calcium- and magnesium-mediated regulation of passive calcium transport across Caco-2 monolayers
    Davies SL, Gibbons CE, Steward MC, Ward DT
    Biochim Biophys Acta. 2008 Oct;1778(10):2318-24

Pricing Top Description

How to order:

  • Select Base Assembly
    Each system includes one or two U9975A Electrode Set(s) and U9965EP Electrode Bridge Fitting Kit(s)
  • Select Insert
    Order 1 if using the U9926 and order 2 if using the U2500
  • Select Data Acquisition package for Ussing (UssingChart)
    If making electrophysiological measurements
  • Select Thermocirculator and Tubing Kit
    If needed - for temperature control

Order No. Model No. Product Description USA Price Order
64-0600 U9924S Culture cup insert for Snapwell, 12.7 mm, 1.12 cm2 area Login Login
64-0598 U9924M-12 Culture cup insert for Millicell adapter, 12.0 mm, 1.13 cm2 area Login Login
64-0601 U9924T-06 Culture cup insert for Transwell adapter 6.5 mm, 0.33 cm2 area Login Login
64-1605 EC-825A Dual channel epithelial voltage clamp, ±50V compliance, 110-250 VAC Login Login
64-3009 EC-825A cable, dual - fits 2 amplifiers (4 acquisition channels) Login Login
89-5035 LIH 8+8 LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE Login Login
89-5244 LIH master/slave connecting cable Login Login
89-5285 UssingChart software Login Login
72-4924 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz Login Login
72-4921 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz Login Login
64-2244 WB-KIT TC-120 Thermocirculator Tubing Kit Login Login
64-0554 U9975A Electrode set, includes 2 Ag/AgCl pellets and 2 Ag wires Login Login
64-0554P Voltage electrodes, Ag/AgCl pellet, 2 mm pin, 4 pack Login Login
64-0554W Current electrodes, Ag wires, 2 mm pin, 4 pack Login Login
64-0555 U9985 Bridge fitting adapters, 6 pack Login Login
64-0565 U9406 Tubing kit (hose and Luer fittings) Login Login
Base Assembly
64-0568S U9926 Single-channel Ussing chamber base assembly, does not include insert Login Login
64-0575S U2500 Dual-channel Ussing chamber base assembly, does not include inserts Login Login
Inserts for tissue with o-ring
64-0582 U9924A-04 Tissue insert with o-ring, 3.8 mm aperture, 0.11 cm2 area Login Login
64-0583 U9924A-06 Tissue insert with o-ring, 6.0 mm aperture, 0.28 cm2 area Login Login
64-0584 U9924A-09 Tissue insert with o-ring, 9.0 mm aperture, 0.64 cm2 area Login Login
64-0585 U9924A-12 Tissue insert with o-ring, 12.0 mm aperture, 1.13 cm2 area Login Login
64-0586 U9924A-13 Tissue insert with o-ring, 13.5 mm aperture, 1.43 cm2 area Login Login
Inserts for tissue with pins
64-0587 U9924B-04 Tissue insert with pins, 3.8 mm aperture, 0.11 cm2 area Login Login
64-0588 U9924B-06 Tissue insert with pins, 6.0 mm aperture, 0.28 cm2 area Login Login
64-0590 U9924B-12 Tissue insert with pins, 12.0 mm aperture, 1.13 cm2 area Login Login
64-0591 U9924B-13 Tissue insert with pins, 13.5 mm aperture, 1.43 cm2 area Login Login
64-0592 U9924C-03 Tissue insert with pins, 1.6 x 7.0 mm aperture, 0.11 cm2 area Login Login
64-0593 U9924C-04 Tissue insert with pins, 2.4 x 7.0 mm aperture, 0.17 cm2 area Login Login
64-0594 U9924C-05 Tissue insert with pins, 3.5 x 7.0 mm aperture, 0.25 cm2 area Login Login
Accessories and Replacement Parts
64-1521 U9565SC Electrode bridge fitting kit (pipette tips and adapters), 12 pack Login Login
64-0604 U9605 O-rings for U9926 and U2500 Ussing chambers, 4 pack Login Login
64-0567 SL-6/U Stopcock with luer fitting for U9926 and U2500, 6 pack Login Login

Need support?

Want to know more about our quality products or need assistance in technical support?

TALK TO OUR EXPERTS

NEWSLETTER

For the most up-to-date information on Warner Instruments news and technologies, sign-up for our newsletter.