For replicate respiration measurements of cell suspensions, microbial suspensions, mitochondria or enzyme preparations.
The RC650 comprises:
The RC650 was designed for use with the 929 Six-Channel Oxygen Meter, for replicate respiration measurements of cell suspensions, microbial suspensions, mitochondria or enzyme preparations. Respiring preparations in metabolic studies are often conducted at 37ºC and have a fast time course. For these studies, it is preferable to use 12.5 µm FEP membranes which have a very fast speed of response (i.e. typically 95% of full response within 6 - 8 secs). These membranes have to be mounted on special electrode jackets (p/n 69-3011) which have a very fine capillary hole opposite the anode. This hole prevents distension of the membrane which would otherwise be caused by pressure building up as the electrode jacket is screwed on.
The respirometer is comprised of a sealed water bath containing both 'active' and 'parking' respirometer cells located above a six-position magnetic stirrer. The six 'active' cells are at the front of the bath and are accurately centered over the coils of the magnetic stirring unit beneath. Each cell is sealed by a neoprene 'O' ring. The volume of the 'active' cells may be set between 1 and 3 mL, by rotating the knurled collar of the electrode holders.
The rear of the bath contains a further six 'parking' cells which are used to park the electrode holders and maintain them at a constant temperature while changing the contents of the respiratory 'active' cells. For easy removal and cleaning the cells are sealed into the water bath by O-rings.
Each electrode holder has an aperture through which the membrane tip of the 1302 Oxygen Sensor protrudes, making a sliding fit into the cells. As the holder is inserted into the preparation, the sloping face at its tip gathers the air into the slot, which runs the complete length of the holder. Respiratory inhibitors or other solutions can be injected directly into the cell via this slot, using a fine bore needle (included).
The electrode holders are made from black acetal. The electrode is positioned in the holder by pressure exerted by the cap on three O rings at the cable-entry end of the electrode. This seals the nose of the electrode against the precision shaped plastic tip of the holder, so that only the membrane-covered cathode protrudes.
|Diameter of Cells||16 mm|
|Volume of Cells||1 to 3 mL (adjustable)|
|Cell||Precision bore glass|
|Electrode Holder||Black acetal, with peek tip|
|Bath||Clear and black acrylic|
|Magnetic Stirrer||6-position; 12 VDC
from external plugtop power supply,
100-230 V, 47-63 Hz.
|Order No.||Model No.||Product Description||USA Price||Order|
|69-3021||RC-650||Six chamber respirometer||Login||Login|
|Accessories and Replacement Parts|
|69-3002||SI-929||Six channel oxygen meter with standard respirometry software||Login||Login|
|69-3006||SI-1302||1302 Oxygen electrode with polyproplyene jackets||Login||Login|
|69-3007||SI-020||Polyproplyene oxygen electrode service kit||Login||Login|
|69-3004||SI-1320F||1302 Oxygen electrode with jacket for FEP membranes||Login||Login|
|69-3005||SI-021||FEP oxygen electrode service kit||Login||Login|
|69-3039||SI-068||Spinbars for MT-200A, MS-200A, RC-650, and RS-650, 6 pack||Login||Login|
|69-3008||SI-030||Electrolyte solution, 125 ml||Login||Login|
|Accessories and Replacement Parts - Thermocirulator|
|72-4924||TC-120||Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz||Login||Login|
|72-4921||TC-120||Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz||Login||Login|
|64-2244||WB-KIT||TC-120 Thermocirculator Tubing Kit||Login||Login|