Specialized Tools for Electrophysiology and Cell Biology Research
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(RC-3Z) Slotted Bath Oocyte Recording Chamber
A simple low-cost chamber for studies of oocytes and other large cell structures.
- Specially designed for oocyte studies
- Features two slot-shaped bath wells
- Allows fast solution exchange and easy cell access
- Adjustable solution height
The RC-3Z features two slotted bath wells; a narrow bath for applications requiring rapid solution exchange, and a wider bath allowing good electrode access.
The chamber is constructed using a two-piece design. The top piece forms the chamber body and contains a solution aspiration reservoir. The bottom piece forms the floor of the chamber and houses the oocyte work area. An agar bridge well with connecting channel to the input side of the bath is also provided. An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.
The perfusion inputs accept standard 1/16" OD (PE-160) polyethylene tubing. Solutions are removed from the suction well via an adjustable suction tube and solution height is set by raising or lowering this suction tube. The suction tube out-port connects to 1/16" ID tubing. Threaded holes are incorporated in the chamber body for mounting of the OC-725Cbath headstage.
Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.
| Chamber material | polycarbonate |
|---|---|
| Footprint (L x W) | 13.0 x 7.7 cm |
| Chamber floor insert | 25 x 75 x 1 mm, polycarbonate |
| Narrow bath dimension (L x W x H) | 27 x 3.2 x 3.2 mm |
| Narrow bath volume, by depth | 85 μl/mm |
| Wide bath dimension (L x W x H) | 27 x 6.3 x 3.2 mm |
| Wide bath volume, by depth | 170 μl/mm |
| Input tubing (ID x OD) | 1.14 x 1.57 mm (PE-160) |
| Aspirator coupling | 1.67 mm (OD) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-0206 | MP-2 | MP Series perfusion minimanifold, 2 to 1 ports | Login | Login |
| 64-0207 | MP-3 | MP Series perfusion minimanifold, 3 to 1 ports | Login | Login |
| 64-0208 | MP-4 | MP Series perfusion minimanifold, 4 to 1 ports | Login | Login |
| 64-0209 | MP-5 | MP Series perfusion minimanifold, 5 to 1 ports | Login | Login |
| 64-0210 | MP-6 | MP Series perfusion mini manifold, 6 to 1 ports | Login | Login |
| 64-0211 | MP-8 | MP Series perfusion mini-manifold, 8 to 1 ports | Login | Login |
| 64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| 64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |
References
References
- A presynaptic endosomal trafficking pathway controls synaptic growth signaling.
Rodal AA, Blunk AD, Akbergenova Y, Jorquera RA, Buhl LK, Littleton JT.
J Cell Biol. 2011 Apr 4;193(1):201-17. - DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization.
Schulte J, Sepp KJ, Jorquera RA, Wu C, Song Y, Hong P, Littleton JT.
J Neurosci. 2010 Apr 14;30(15):5189-203. - Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains.
Barber CF, Jorquera RA, Melom JE, Littleton JT.
J Cell Biol. 2009 Oct 19;187(2):295-310. Epub 2009 Oct 12. - Oviduct contraction in Drosophila is modulated by a neural network that is both, octopaminergic and glutamatergic.
Rodríguez-Valentín R, López-González I, Jorquera R, Labarca P, Zurita M, Reynaud E.
J Cell Physiol. 2006 Oct;209(1):183-98. - Drosophila melanogaster Scramblases modulate synaptic transmission.
Acharya U, Edwards MB, Jorquera RA, Silva H, Nagashima K, Labarca P, Acharya JK.
J Cell Biol. 2006 Apr 10;173(1):69-82. - Ligand-Gated Chloride Channels Are Receptors for Biogenic Amines in C. elegans
Ringstad N, Abe N, Horvitz HR
Science. 2009 Jul 3;325(5936):96-100 - Five different profiles of dihydropyridines in blocking T-type Ca2+ channel subtypes (Cav3.1 (?1G), Cav3.2 (?1H), and Cav3.3 (?1I)) expressed in Xenopus oocytes
Taiji Furukawaa, Toshihide Nukadab, Yoshiko Namikib, Yoriko Miyashitaa, Kento Hatsunoa, Yasunari Uenoa, Takeshi Yamakawaa and TakaakiIsshiki
European Journal of Pharmacology Volume 613, Issues 1-3, 24 June 2009, Pages 100-107 - Roles of ectodomain and transmembrane regions in ethanol and agonist action in purinergic P2X2 and P2X3 receptors
Asatryan L, Popova M, Woodward JJ, King BF, Alkana RL, Davies DL
Neuropharmacology. 2008 Oct;55(5):835-43 - ?4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors
Peng C, Han Y, Sanders T, Chew G, Liu J, Hawrot E, Chi C, Wang C
Peptides. 2008 Oct;29(10):1700-7 - The TRPV3 mutation associated with the hairless phenotype in rodents is constitutively active
Xiao R, Tian J, Tang J, Zhu MX
Cell Calcium. 2008 Apr;43(4):334-43 - Modulation of the heteromeric Kir4.1-Kir5.1 channel by multiple neurotransmitters via Gq-coupled receptors
Rojas A, Su J, Yang L, Lee M, Cui N, Zhang X, Fountain D, Jiang C
J Cell Physiol. 2008 Jan;214(1):84-95 - 1,4-Diazabicyclo[2.2.2]octane Derivatives: A Novel Class of Voltage-Gated Potassium Channel Blockers
Gordon E, Cohen JL, Engel R, Abbott GW
Mol Pharmacol. 2006 Mar;69(3):718-26 - Epithelial Sodium Channel Inhibition by AMP-activated Protein Kinase in Oocytes and Polarized Renal Epithelial Cells*
Carattino MD, Edinger RS, Grieser HJ, Wise R, Neumann D, Schlattner U, Johnson JP, Kleyman TR, Hallows KR
J Biol Chem. 2005 May 6;280(18):17608-16
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-0319 | RC-3Z | RC-3Z oocyte recording chamber | Login | Login |
| Accessories and Replacement Parts | ||||
| 64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
| 64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |
| 64-0206 | MP-2 | MP Series perfusion minimanifold, 2 to 1 ports | Login | Login |
| 64-0207 | MP-3 | MP Series perfusion minimanifold, 3 to 1 ports | Login | Login |
| 64-0208 | MP-4 | MP Series perfusion minimanifold, 4 to 1 ports | Login | Login |
| 64-0209 | MP-5 | MP Series perfusion minimanifold, 5 to 1 ports | Login | Login |
| 64-0210 | MP-6 | MP Series perfusion mini manifold, 6 to 1 ports | Login | Login |
| 64-0211 | MP-8 | MP Series perfusion mini-manifold, 8 to 1 ports | Login | Login |
| 64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
| Plugs and Jacks | ||||
| 64-1327 | WA10-5 | Silver wire, 10 cm long, 0.25 mm dia, with 1 mm pin, 2 pack | Login | Login |
| Valve Control Systems | ||||
| 64-0185 | VC-8P | Perfusion valve control system, complete, with pinch valves, 8 channel | Login | Login |
| 64-0135 | VC-66CS | Perfusion valve control system, complete, with pinch valves, 6 channel | Login | Login |
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |