Specialized Tools for Electrophysiology and Cell Biology Research

(RC-3Z) Slotted Bath Oocyte Recording Chamber

A simple low-cost chamber for studies of oocytes and other large cell structures.

  • Specially designed for oocyte studies
  • Features two slot-shaped bath wells
  • Allows fast solution exchange and easy cell access
  • Adjustable solution height

The RC-3Z features two slotted bath wells; a narrow bath for applications requiring rapid solution exchange, and a wider bath allowing good electrode access.

The chamber is constructed using a two-piece design. The top piece forms the chamber body and contains a solution aspiration reservoir. The bottom piece forms the floor of the chamber and houses the oocyte work area. An agar bridge well with connecting channel to the input side of the bath is also provided. An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.

 

The perfusion inputs accept standard 1/16" OD (PE-160) polyethylene tubing. Solutions are removed from the suction well via an adjustable suction tube and solution height is set by raising or lowering this suction tube. The suction tube out-port connects to 1/16" ID tubing. Threaded holes are incorporated in the chamber body for mounting of the OC-725Cbath headstage.

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

 

Chamber material polycarbonate
Footprint (L x W) 13.0 x 7.7 cm
Chamber floor insert 25 x 75 x 1 mm, polycarbonate
Narrow bath dimension (L x W x H) 27 x 3.2 x 3.2 mm
Narrow bath volume, by depth 85 μl/mm
Wide bath dimension (L x W x H) 27 x 6.3 x 3.2 mm
Wide bath volume, by depth 170 μl/mm
Input tubing (ID x OD) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)
Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0206 MP-2 MP Series perfusion minimanifold, 2 to 1 ports Login Login
64-0207 MP-3 MP Series perfusion minimanifold, 3 to 1 ports Login Login
64-0208 MP-4 MP Series perfusion minimanifold, 4 to 1 ports Login Login
64-0209 MP-5 MP Series perfusion minimanifold, 5 to 1 ports Login Login
64-0210 MP-6 MP Series perfusion mini manifold, 6 to 1 ports Login Login
64-0211 MP-8 MP Series perfusion mini-manifold, 8 to 1 ports Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1586 BPM-1 Base plate, magnetic steel, 12x24" x 3/8', thick powder coating Login Login

References

References

  1. A presynaptic endosomal trafficking pathway controls synaptic growth signaling.
    Rodal AA, Blunk AD, Akbergenova Y, Jorquera RA, Buhl LK, Littleton JT.
    J Cell Biol. 2011 Apr 4;193(1):201-17.
  2. DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization.
    Schulte J, Sepp KJ, Jorquera RA, Wu C, Song Y, Hong P, Littleton JT.
    J Neurosci. 2010 Apr 14;30(15):5189-203.
  3. Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains.
    Barber CF, Jorquera RA, Melom JE, Littleton JT.
    J Cell Biol. 2009 Oct 19;187(2):295-310. Epub 2009 Oct 12.
  4. Oviduct contraction in Drosophila is modulated by a neural network that is both, octopaminergic and glutamatergic.
    Rodríguez-Valentín R, López-González I, Jorquera R, Labarca P, Zurita M, Reynaud E.
    J Cell Physiol. 2006 Oct;209(1):183-98.
  5. Drosophila melanogaster Scramblases modulate synaptic transmission.
    Acharya U, Edwards MB, Jorquera RA, Silva H, Nagashima K, Labarca P, Acharya JK.
    J Cell Biol. 2006 Apr 10;173(1):69-82.
  6. Ligand-Gated Chloride Channels Are Receptors for Biogenic Amines in C. elegans
    Ringstad N, Abe N, Horvitz HR
    Science. 2009 Jul 3;325(5936):96-100
  7. Five different profiles of dihydropyridines in blocking T-type Ca2+ channel subtypes (Cav3.1 (?1G), Cav3.2 (?1H), and Cav3.3 (?1I)) expressed in Xenopus oocytes
    Taiji Furukawaa, Toshihide Nukadab, Yoshiko Namikib, Yoriko Miyashitaa, Kento Hatsunoa, Yasunari Uenoa, Takeshi Yamakawaa and TakaakiIsshiki
    European Journal of Pharmacology Volume 613, Issues 1-3, 24 June 2009, Pages 100-107
  8. Roles of ectodomain and transmembrane regions in ethanol and agonist action in purinergic P2X2 and P2X3 receptors
    Asatryan L, Popova M, Woodward JJ, King BF, Alkana RL, Davies DL
    Neuropharmacology. 2008 Oct;55(5):835-43
  9. ?4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors
    Peng C, Han Y, Sanders T, Chew G, Liu J, Hawrot E, Chi C, Wang C
    Peptides. 2008 Oct;29(10):1700-7
  10. The TRPV3 mutation associated with the hairless phenotype in rodents is constitutively active
    Xiao R, Tian J, Tang J, Zhu MX
    Cell Calcium. 2008 Apr;43(4):334-43
  11. Modulation of the heteromeric Kir4.1-Kir5.1 channel by multiple neurotransmitters via Gq-coupled receptors
    Rojas A, Su J, Yang L, Lee M, Cui N, Zhang X, Fountain D, Jiang C
    J Cell Physiol. 2008 Jan;214(1):84-95
  12. 1,4-Diazabicyclo[2.2.2]octane Derivatives: A Novel Class of Voltage-Gated Potassium Channel Blockers
    Gordon E, Cohen JL, Engel R, Abbott GW
    Mol Pharmacol. 2006 Mar;69(3):718-26
  13. Epithelial Sodium Channel Inhibition by AMP-activated Protein Kinase in Oocytes and Polarized Renal Epithelial Cells*
    Carattino MD, Edinger RS, Grieser HJ, Wise R, Neumann D, Schlattner U, Johnson JP, Kleyman TR, Hallows KR
    J Biol Chem. 2005 May 6;280(18):17608-16
Order No. Model No. Product Description USA Price Order
64-0319 RC-3Z RC-3Z oocyte recording chamber Login Login
Accessories and Replacement Parts
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-1586 BPM-1 Base plate, magnetic steel, 12x24" x 3/8', thick powder coating Login Login
64-0206 MP-2 MP Series perfusion minimanifold, 2 to 1 ports Login Login
64-0207 MP-3 MP Series perfusion minimanifold, 3 to 1 ports Login Login
64-0208 MP-4 MP Series perfusion minimanifold, 4 to 1 ports Login Login
64-0209 MP-5 MP Series perfusion minimanifold, 5 to 1 ports Login Login
64-0210 MP-6 MP Series perfusion mini manifold, 6 to 1 ports Login Login
64-0211 MP-8 MP Series perfusion mini-manifold, 8 to 1 ports Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Plugs and Jacks
64-1327 WA10-5 Silver wire, 10 cm long, 0.25 mm dia, with 1 mm pin, 2 pack Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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