A simple low-cost chamber for studies of oocytes and other large cell structures.
The RC-3Z features two slotted bath wells; a narrow bath for applications requiring rapid solution exchange, and a wider bath allowing good electrode access.
The chamber is constructed using a two-piece design. The top piece forms the chamber body and contains a solution aspiration reservoir. The bottom piece forms the floor of the chamber and houses the oocyte work area. An agar bridge well with connecting channel to the input side of the bath is also provided. An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.
The perfusion inputs accept standard 1/16" OD (PE-160) polyethylene tubing. Solutions are removed from the suction well via an adjustable suction tube and solution height is set by raising or lowering this suction tube. The suction tube out-port connects to 1/16" ID tubing. Threaded holes are incorporated in the chamber body for mounting of the OC-725D bath headstage.
Perfusion control can be achieved by adding a VCS Perfusion System, the DVW vacuum system takes care of the waste.
Chamber material | polycarbonate |
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Footprint (L x W) | 13.0 x 7.7 cm |
Chamber floor insert | 25 x 75 x 1 mm, polycarbonate |
Narrow bath dimension (L x W x H) | 27 x 3.2 x 3.2 mm |
Narrow bath volume, by depth | 85 μl/mm |
Wide bath dimension (L x W x H) | 27 x 6.3 x 3.2 mm |
Wide bath volume, by depth | 170 μl/mm |
Input tubing (ID x OD) | 1.14 x 1.57 mm (PE-160) |
Aspirator coupling | 1.67 mm (OD) |
Order No. | Model No. | Product Description | USA Price | Order |
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64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
64-0206 | MP-2 | MP Series perfusion mini manifold, 2 to 1 ports | Login | Login |
64-0207 | MP-3 | MP Series perfusion mini manifold, 3 to 1 ports | Login | Login |
64-0208 | MP-4 | MP Series perfusion mini manifold, 4 to 1 ports | Login | Login |
64-0209 | MP-5 | MP Series perfusion minimanifold, 5 to 1 ports | Login | Login |
64-0210 | MP-6 | MP Series perfusion mini manifold, 6 to 1 ports | Login | Login |
64-0211 | MP-8 | MP Series perfusion mini-manifold, 8 to 1 ports | Login | Login |
64-0106 | CC-28 | Cable assembly for heater controllers to platform | Login | Login |
64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |
References
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
Order No. | Model No. | Product Description | USA Price | Order |
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64-0319 | RC-3Z | RC-3Z oocyte recording chamber | Login | Login |
Accessories and Replacement Parts | ||||
64-0755 | PE-160/10 | Polyethylene tubing, PE-160, 10 ft | Login | Login |
64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |
64-0206 | MP-2 | MP Series perfusion mini manifold, 2 to 1 ports | Login | Login |
64-0207 | MP-3 | MP Series perfusion mini manifold, 3 to 1 ports | Login | Login |
64-0208 | MP-4 | MP Series perfusion mini manifold, 4 to 1 ports | Login | Login |
64-0209 | MP-5 | MP Series perfusion minimanifold, 5 to 1 ports | Login | Login |
64-0210 | MP-6 | MP Series perfusion mini manifold, 6 to 1 ports | Login | Login |
64-0211 | MP-8 | MP Series perfusion mini-manifold, 8 to 1 ports | Login | Login |
64-0107 | TA-29 | Cable with bead thermistor | Login | Login |
64-1327 | WA10-5 | Silver wire, 10 cm long, 0.25 mm dia, with 1 mm pin, 2 pack | Login | Login |
Perfusion and Waste Management | ||||
64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |