Specialized Tools for Electrophysiology and Cell Biology Research
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(TEV-700) Two Electrode Voltage Clamp Workstation
Product Summary
The TEV-700 Oocyte Workstation provides most everything needed for patch clamp research involving oocytes.
The Workstation includes:
- OC-725D Oocyte clamp amplifier
- RC-3Z Oocyte recording chamber
- MM-33L left handed micromanipulator
- MM-33R right handed micromanipulator
- MB/B magnetic base (2 in complete system)
Voltage and current electrode holders purchased separately
Notes on Electrode Holders
Two vented electrode holders with silver wires are typically used with the clamp; a straight ESW Series holder for use with the voltage headstage, and a 45° angled E45W Series holder (with mounting handle) for use with current electrode. Vented electrode holders are preferred so as to prevent pressure build-up which can damage the oocytes. Select holders based on your glass outer diameter (OD). If needed, unvented holders are also available A two meter cable is provided to connect the current electrode holder to the clamp.
Accessories
The following accessories are recommended to either complete the workstation or to improve its functionality.
BPM-1 Baseplate
This solid steel plate provides a stable platform for housing the Workstation and for mounting micromanipulators mounted on magnetic bases. The highly durable powder coating applied to the BPM surface makes it impervious to most common lab spills.
Microscope
The Z850 is a versatile and high performance stereo zoom microscope ruggedly built to withstand years of heavy usage. Offered with ball bearing boom stand.
Cold light source
The F3000 fiber optic illuminator from Photonic delivers high-performance LED illumination for the most demanding applications. The optional dual gooseneck lightguide provides good lighting of the oocyte while working and viewing through a microscope.
Data Acquisition System
The ChartMaster software is designed with the objective of streamlining data collection, data presentation, and data analysis, both during and after an experiment. The software's versatile design allows it to be used for any general purpose data acquisition need.
OC-725D Specifications
| Test Condition #1 | Model cell used to obtain specifications. Model membrane: 1 MΩ in parallel with 550 nF. Current and voltage electrodes: both 1 MΩ impedance. |
|
|---|---|---|
| Test Condition #2 | Noise measurements made with an 8-pole Bessel filter. | |
| Voltage Recording Channel (Vm) | V Probe Input Impedance | 0.5 x 1012Ω, 1 pF |
| Output Resistance | 100 Ω | |
| DC Offset | ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn). | |
| Noise (0-10 kHz) | 3 µV RMS with input grounded 20 µV RMS with model cell |
|
| Electrode Test | 10 mV/MΩ read on meter 100 mV/MΩ at Vm x10 output |
|
| Meter Range | ± 199.9 mV full scale | |
| Current Sensing Channel (Bath Clamp) (Im) | Noise (0-1 kHz) | 4.5 nA RMS with bath clamp 10 nA RMS in output leg |
| I Monitor Output | 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10. | |
| Gain Telegraph Output | 0.2 to 2.8 V in 8 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software |
|
| Meter Range, full scale | Clamp Current: ± 199.9 µA | |
| Electrode Voltage (Ve) | ± 199.9 mV; current meter reads Ve when clamp mode switch is off. | |
| Current Electrode Channel | Compliance Voltage | ± 180 V |
| Clamp Speed | 550 µsec; (10-90%) with 100 mV square wave command applied to model cell | |
| Gain | Variable AC/DC: 0 to 2000 | |
| Fixed DC Gain | Switch selected: 1 x 106 | |
| Ve DC Offset | ± 200 mV at input (20 mV/turn) | |
| Electrode Test | 10 mV/MΩ read on current meter 100 mV/MΩ at Ve x10 output rear panel |
|
| Commands | Hold | Manually set with digital potentiometer, 2 digit resolution and 2 ranges: x10 range: 0 to ± 100 mV x20 range: 0 to ± 200 mV |
| External | Signals applied to COMMAND IN÷10 are attenuated by a factor of 10, 1 V applied = 100 mV command |
|
| Physical Dimensions | Case | 8.9 cm x 43.2 cm x 30.5 cm (H x W x D) |
| Voltage Headstage | 12.5 mm D x 5 cm L with 1.8 m cable | |
| Mounting Handle | 8.0 mm D x 6.3 cm L | |
| Bath Headstage | 2.3 cm x 3.5 cm x 4.2 cm (H x W x D), with 1.8 m cable | |
| Shipping Weight | 6.0 kg (13 lb) | |
| Warranty | One year, parts & labor | |
| Power Requirements | Universal: 100-130 or 220-240 VAC, 50/60 Hz, 14 VA | |
RC-3Z Oocyte Recording Chamber Specifications
| Material | Polycarbonate |
| Footprint, L x W | 13 x 7.7 cm (5.1 x 3 in) |
| Narrow Bath Dimension | 27 x 3.2 x 3.2 mm |
| Narrow Bath Volume | ~85 µl/mm height |
| Wide Bath Dimension | 27 x 6.3 x 3.2 mm |
| Wide Bath Volume | ~170 µl/mm height |
| Chamber Floor Insert | 25 x 75 x 1 mm, polycarbonate |
| Input Tubing Size | 1/16 in OD (PE-160) |
| Output Tubing Size | 1/16 in OD (PE-160) |
BPM-1 Specifications
| Dimensions | 30 x 60 x 1 cm (12 x 24 x 3/8 in ) |
| Material | Powder coated magnetic stainless steel |
| Shipping Weight | 18.5 kg (41 lb) |
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |
| 69-3751 | 4/555 DGN | Dual arm gooseneck fiber optic light guide for F3000 light source, 4.5 mm dia, 550 mm (21") long each arm | Login | Login |
| 69-0229 | MB-W2S | Magnetic base, main pole (20 mm D), 1st Sub Pole (14 mm D), 2nd sub pole (12 mm D), 1000 N hold force, not MM-33 compatible | Login | Login |
| 89-5035 | LIH 8+8 | LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE | Login | Login |
References
Videos
- On-Demand Webinar: Data Acquisition and Recording with TEV-700 Oocyte Workstation
Warner Instruments
Vimeo 2013
References
- Maprotiline Block of the Human Ether-a-go-go-related Gene (HERG) K+ Channel
Su-Hyun Jo, Hee-Kyung Hong, Sung Jun Jung, Seon Ha Chong1, Ji-Hyun Yun, Young-Sang Koh, and Han Choe1
Arch Pharm Res Vol 30, No 4, 453-460, 2007 - A Simple, Cross-linked Collagen Tissue Substitute for Corneal Implantation
Liu Y, Gan L, Carlsson DJ, Fagerholm P, Lagali N, Watsky MA, Munger R, Hodge WG, Priest D, Griffith M
Invest Ophthalmol Vis Sci. 2006 May;47(5):1869-75 - The cholinergic antagonist bungarotoxin also binds and blocks a subset of GABA receptors
McCann CM, Bracamontes J, Steinbach JH, Sanes JR
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5149-54 - The anxioselective agent 7-(2-chloropyridin-4-yl)pyrazolo-[1,5-a]-pyrimidin-3-yl](pyridin-2-yl)methanone (DOV 51892) is more efficacious than diazepam at enhancing GABA-gated currents at alpha1 subunit-containing GABAA receptors
Piotr Popik, Emmanuel Kostakis, Martyna Krawczyk, Gabriel Nowak, Bernadeta Szewczyk, Philip Krieter, Zhengming Chen, Shelley J. Russek, Terrell T. Gibbs, David H. Farb, Phil Skolnick, Arnold S. Lippa, and Anthony S. Basile
JPET Fast Forward. Published on September 13, 2006 - PMA- and ANG II-induced PKC regulation of the renal Na -HCO3 cotransporter (hkNBCe1)
Clint Perry, Judith Blaine, Hong Le and Irina I. Grichtchenko
Am J Physiol Renal Physiol 290:417-427, 2006 - Chemical Modification of the Human Ether-a-go-go-related gene (HERG) K+ Current by the Amino-Group Reagent Trinitrobenzene Sulfonic Acid
Su-Hyun Jo1, Se-Young Choi, Ji-Hyun Yun4, Young-Sang Koh, Won-Kyung Ho5, and Chin O. Lee
Arch Pharm Res Vol 29, No 4, 310-317, 2006 - Oxidation and Reduction Control of the Inactivation Gating of Torpedo ClC-0 Chloride Channels
Yong Li, Wei-Ping Yu, Chia-Wei Lin, and Tsung-Yu Chen
Biophysical Journal Volume 88 June 2005 3936–3945 - Inhibition of Human ether-a-go-go-Related Gene K+ Channel and IKr of Guinea Pig Cardiomyocytes by Antipsychotic Drug Trifluoperazine
Se-Young Choi, Young-Sang Koh, and Su-Hyun Jo
JPET 313:888-895, 2005
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
| Order No. | Model No. | Product Description | USA Price | Order |
|---|---|---|---|---|
| 64-3071 | TEV-700D | Complete oocyte clamp workstation | Login | Login |
| Data Acquisition | ||||
| 89-5245 | PatchMaster Next | Login | Login | |
| 64-0050 | LPF-8 | Low pass bessel filter, 8-pole, with differential amplifier, 110-240 VAC, 50/60 Hz | Login | Login |
| 89-5035 | LIH 8+8 | LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE | Login | Login |
| Electrode Holders for OC-725C Current Electrode (Purchased Separately) | ||||
| 64-1051 | E45W-F10VH | E Series Electrode Holder, 45° with handle, fits 1.0 mm capillary, Ag wire | Login | Login |
| 64-1052 | E45W-F12VH | E Series Electrode Holder, 45° with handle, fits 1.2 mm capillary, Ag wire | Login | Login |
| 64-1053 | E45W-F15VH | E Series Electrode Holder, 45° with handle, fits 1.5 mm capillary, Ag wire | Login | Login |
| 64-1054 | E45W-F20VH | E Series Electrode Holder, 45° with handle, fits 2.0 mm capillary, Ag wire | Login | Login |
| Electrode Holders for OC-725C Voltage Probe (Purchased Separately) | ||||
| 64-1007 | ESW-F10V | E Series Electrode Holder, straight style, fits 1.0 mm capillary, Ag wire | Login | Login |
| 64-1008 | ESW-F12V | E Series Electrode Holder, straight style, fits 1.2 mm capillary, Ag wire | Login | Login |
| 64-1009 | ESW-F15V | E Series Electrode Holder, straight style, fits 1.5 mm capillary, Ag wire | Login | Login |
| 64-1010 | ESW-F20V | E Series Electrode Holder, straight style, fits 2.0 mm capillary, Ag wire | Login | Login |
| Workstation Components | ||||
| 64-3068 | OC-725D | Oocyte Voltage Clamp Amplifier | Login | Login |
| 64-0319 | RC-3Z | RC-3Z oocyte recording chamber | Login | Login |
| 64-0056 | MM-33R | Micromanipulator with clamp base, right handed | Login | Login |
| 64-0055 | MM-33L | Micromanipulator with clamp base, left handed | Login | Login |
| Accessories | ||||
| 64-1835 | Binocular stereo microscope, 8-50x zoom, ball bearing boom stand, includes 10X eyepieces | Login | Login | |
| 69-4033 | F3000 | F3000 LED Cold light source - US power cord | Login | Login |
| 69-3751 | 4/555 DGN | Dual arm gooseneck fiber optic light guide for F3000 light source, 4.5 mm dia, 550 mm (21") long each arm | Login | Login |
| 64-0060 | MB-B | Magnetic base; main pole (12 x 176 mm, D x L), Sub Pole (10 x 165 mm, D x L), 800 N hold force, MM-33 compatible | Login | Login |
| 64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
| 64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |