The TEV-700 Oocyte Workstation provides most everything needed for patch clamp research involving oocytes.
Voltage and current electrode holders purchased separately
Two vented electrode holders with silver wires are typically used with the clamp; a straight ESW Series holder for use with the voltage headstage, and a 45° angled E45W Series holder (with mounting handle) for use with current electrode. Vented electrode holders are preferred so as to prevent pressure build-up which can damage the oocytes. Select holders based on your glass outer diameter (OD). If needed, unvented holders are also available A two meter cable is provided to connect the current electrode holder to the clamp.
The following accessories are recommended to either complete the workstation or to improve its functionality.
BPM-1 Baseplate
This solid steel plate provides a stable platform for housing the Workstation and for mounting micromanipulators mounted on magnetic bases. The highly durable powder coating applied to the BPM surface makes it impervious to most common lab spills.
Microscope
The Z850 is a versatile and high performance stereo zoom microscope ruggedly built to withstand years of heavy usage. Offered with ball bearing boom stand.
Cold light source
The F3000 fiber optic illuminator from Photonic delivers high-performance LED illumination for the most demanding applications. The optional dual gooseneck lightguide provides good lighting of the oocyte while working and viewing through a microscope.
Test Condition #1 | Model cell used to obtain specifications. Model membrane: 1 MΩ in parallel with 550 nF. Current and voltage electrodes: both 1 MΩ impedance. |
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Test Condition #2 | Noise measurements made with an 8-pole Bessel filter. | |
Voltage Recording Channel (Vm) | V Probe Input Impedance | 0.5 x 1012Ω, 1 pF |
Output Resistance | 100 Ω | |
DC Offset | ± 200 mV at input. Variations from zero with 10 turn control (20 mV/turn). | |
Noise (0-10 kHz) | 3 µV RMS with input grounded 20 µV RMS with model cell |
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Electrode Test | 10 mV/MΩ read on meter 100 mV/MΩ at Vm x10 output |
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Meter Range | ± 199.9 mV full scale | |
Current Sensing Channel (Bath Clamp) (Im) | Noise (0-1 kHz) | 4.5 nA RMS with bath clamp 10 nA RMS in output leg |
I Monitor Output | 1 nA/mV to 1 mA/10V in 7 steps and 3 ranges, x0.1, x1, and x10. | |
Gain Telegraph Output | 0.2 to 2.8 V in 8 steps (200 mV/step) and 3 ranges, x0.1, x1, and x10. Compatible with data acquisition software |
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Meter Range, full scale | Clamp Current: ± 199.9 µA | |
Electrode Voltage (Ve) | ± 199.9 mV; current meter reads Ve when clamp mode switch is off. | |
Current Electrode Channel | Compliance Voltage | ± 180 V |
Clamp Speed | 550 µsec; (10-90%) with 100 mV square wave command applied to model cell | |
Gain | Variable AC/DC: 0 to 2000 | |
Fixed DC Gain | Switch selected: 1 x 106 | |
Ve DC Offset | ± 200 mV at input (20 mV/turn) | |
Electrode Test | 10 mV/MΩ read on current meter 100 mV/MΩ at Ve x10 output rear panel |
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Commands | Hold | Manually set with digital potentiometer, 2 digit resolution and 2 ranges: x10 range: 0 to ± 100 mV x20 range: 0 to ± 200 mV |
External | Signals applied to COMMAND IN÷10 are attenuated by a factor of 10, 1 V applied = 100 mV command |
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Physical Dimensions | Case | 8.9 cm x 43.2 cm x 30.5 cm (H x W x D) |
Voltage Headstage | 12.5 mm D x 5 cm L with 1.8 m cable | |
Mounting Handle | 8.0 mm D x 6.3 cm L | |
Bath Headstage | 2.3 cm x 3.5 cm x 4.2 cm (H x W x D), with 1.8 m cable | |
Shipping Weight | 6.0 kg (13 lb) | |
Warranty | One year, parts & labor | |
Power Requirements | Universal: 100-130 or 220-240 VAC, 50/60 Hz, 14 VA |
Material | Polycarbonate |
Footprint, L x W | 13 x 7.7 cm (5.1 x 3 in) |
Narrow Bath Dimension | 27 x 3.2 x 3.2 mm |
Narrow Bath Volume | ~85 µl/mm height |
Wide Bath Dimension | 27 x 6.3 x 3.2 mm |
Wide Bath Volume | ~170 µl/mm height |
Chamber Floor Insert | 25 x 75 x 1 mm, polycarbonate |
Input Tubing Size | 1/16 in OD (PE-160) |
Output Tubing Size | 1/16 in OD (PE-160) |
Dimensions | 30 x 60 x 1 cm (12 x 24 x 3/8 in ) |
Material | Powder coated magnetic stainless steel |
Shipping Weight | 18.5 kg (41 lb) |
Order No. | Model No. | Product Description | USA Price | Order |
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64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |
69-3751 | 4/555 DGN | Dual arm gooseneck fiber optic light guide for F3000 light source, 4.5 mm dia, 550 mm (21") long each arm | Login | Login |
69-0229 | MB-W2S | Magnetic base, main pole (20 mm D), 1st Sub Pole (14 mm D), 2nd sub pole (12 mm D), 1000 N hold force, not MM-33 compatible | Login | Login |
89-5035 | LIH 8+8 | LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE | Login | Login |
Videos
References
Customers from Europe and Australia can use the Multi Channel Systems - Online Shop to order Warner products or create quotes.
Order No. | Model No. | Product Description | USA Price | Order |
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64-3071 | TEV-700D | Complete oocyte clamp workstation | Login | Login |
Data Acquisition | ||||
89-5245 | PatchMaster Next | Login | Login | |
64-0050 | LPF-8 | Low pass bessel filter, 8-pole, with differential amplifier, 110-240 VAC, 50/60 Hz | Login | Login |
89-5035 | LIH 8+8 | LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE | Login | Login |
Electrode Holders for OC-725C Current Electrode (Purchased Separately) | ||||
64-1051 | E45W-F10VH | E Series Electrode Holder, 45° with handle, fits 1.0 mm capillary, Ag wire | Login | Login |
64-1052 | E45W-F12VH | E Series Electrode Holder, 45° with handle, fits 1.2 mm capillary, Ag wire | Login | Login |
64-1053 | E45W-F15VH | E Series Electrode Holder, 45° with handle, fits 1.5 mm capillary, Ag wire | Login | Login |
64-1054 | E45W-F20VH | E Series Electrode Holder, 45° with handle, fits 2.0 mm capillary, Ag wire | Login | Login |
Electrode Holders for OC-725C Voltage Probe (Purchased Separately) | ||||
64-1007 | ESW-F10V | E Series Electrode Holder, straight style, fits 1.0 mm capillary, Ag wire | Login | Login |
64-1008 | ESW-F12V | E Series Electrode Holder, straight style, fits 1.2 mm capillary, Ag wire | Login | Login |
64-1009 | ESW-F15V | E Series Electrode Holder, straight style, fits 1.5 mm capillary, Ag wire | Login | Login |
64-1010 | ESW-F20V | E Series Electrode Holder, straight style, fits 2.0 mm capillary, Ag wire | Login | Login |
Workstation Components | ||||
64-3068 | OC-725D | Oocyte Voltage Clamp Amplifier | Login | Login |
64-0319 | RC-3Z | RC-3Z oocyte recording chamber | Login | Login |
64-0056 | MM-33R | Micromanipulator with clamp base, right handed | Login | Login |
64-0055 | MM-33L | Micromanipulator with clamp base, left handed | Login | Login |
Accessories | ||||
64-1835 | Binocular stereo microscope, 8-50x zoom, ball bearing boom stand, includes 10X eyepieces | Login | Login | |
69-4033 | F3000 | F3000 LED Cold light source - US power cord | Login | Login |
69-3751 | 4/555 DGN | Dual arm gooseneck fiber optic light guide for F3000 light source, 4.5 mm dia, 550 mm (21") long each arm | Login | Login |
64-0060 | MB-B | Magnetic base; main pole (12 x 176 mm, D x L), Sub Pole (10 x 165 mm, D x L), 800 N hold force, MM-33 compatible | Login | Login |
64-1940 | DWV | Dedicated Workstation Vacuum | Login | Login |
64-1586 | BPM-1 | Base plate, magnetic steel, 12x24" x 3/8', thick powder coating | Login | Login |