Specialized Tools for Electrophysiology and Cell Biology Research

(RC-29) Very Large Rectangular Open Bath for Slice Studies

Product Summary

The RC-29 is designed for slice studies requiring controlled atmospheric conditions.

  • Designed for tissue slice studies
  • Gas perfusion ports allow for controlled atmospheric conditions
  • Provides for perfusate flow both above and below a tissue slice
  • Provides good access for immersion optics and electrodes
  • Can be used with either upright or inverted microscopes
  • Large imaging area
  • Slice anchors available

The RC-29 chamber is modeled after the RC-27L (in that it enables solution flow both above and below the tissue slice) with the added feature of atmospheric environmental control.

Similar to the RC-27L, the tissue slice rests on a plastic slice support 0.5 mm above the coverslip floor and is held in place by an optional slice anchor. Slice supports and slice anchors use fine Lycra threads to secure the slice into place allowing maximum access to the preparation. Thread spacing is 1 mm on the slice support and are 1.0, 1.5, and 2.0 mm for the slice anchors. Anchors are purchased separately and are available in polycarbonate and stainless steel. Warner also offers anchor kits containing slice anchors (1 each) in each available thread spacing.

The RC-29 has tall sides with sloping surfaces to allow for an atmospheric space. Gas input ports are constructed in the chamber walls and a chamber top, with access port, is also provided. Continuous gas flow into the chamber establishes positive pressure within the chamber maintaining the atmospheric environment.

This chamber requires a platform (PM-6DPH-6DP-6D) and Series 20 stage adapter to complete the assembly for mounting on a microscope. The chamber bottom is formed by a 24 x 50 mm coverslip.

 

The RC-29 chamber is supplied with a plastic SS-3 slice support, 10 ft of PE-160 polyethylene tubing, a chamber cover, and one pack of #1 thickness coverslips (CS-24/50, 24 x 50 mm, pkg. of 50).

Material Polycarbonate
Bath dimensions
(L x W x H)
38 x 18 x 6 mm
Outer dimensions
(L x W x H)
50 x 25 x 8 mm)
Volume, by depth 629 μl/mm
Bottom coverglass 24 x 50 mm
Slice support 24 x 50 mm, polycarbonate
Lycra threads at 1 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)
Gas perfusion ports Stainless steel, 1.67 mm (OD)


Order No. Model No. Product Description USA Price Order
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0273 SS-3 Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1533 SS-3G Mesh slice support for RC-27L, RC-29 recording chambers Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Real-Time Passive Volume Responses of Astrocytes to Acute Osmotic and Ischemic Stress in Cortical Slices and in vivo Revealed by Two-Photon Microscopy
    Risher WC, Andrew RD, Kirov SA
    Glia. 2009 Jan 15;57(2):207-21
  2. PI3K integrates the action of insulin and leptin on hypothalamic neurons,
    Allison Wanting Xu, Christopher B. Kaelin1, Kiyoshi Takeda, Shizuo Akira, Michael W. Schwartz and Gregory S. Barsh1
    J. Clin. Invest. 115:951-958 (2005)

Order No. Model No. Product Description USA Price Order
Chamber
64-0244 RC-29 Very large bath chamber, does not include slice hold down Login Login
Platforms
64-1528 PM-6D PM-6D Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-0290 PH-6D PH-6D Platform for Series 20 chambers, heater Login Login
64-0283 P-6D P-6D Platform for Series 20 chambers, non-heater Login Login
Slice Anchors (Harps)
64-0268 SHD-27LKIT Slice anchor kit for RC-27L, RC-27D, RC-29, stainless steel Login Login
64-0256 SHD-27LH/10 Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, stainless steel Login Login
64-0257 SHD-27LH/15 Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, stainless steel Login Login
64-0258 SHD-27LH/2 Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, stainless steel Login Login
Slice Anchors (Harps) - Plastic Frame
64-0190 SHD-27LPKIT Slice anchor kit for RC-27L, RC-27D, RC-29, plastic frame Login Login
64-0259 SHD-27LP/10 Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, plastic frame Login Login
64-0260 SHD-27LP/15 Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, plastic frame Login Login
64-0261 SHD-27LP/2 Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, plastic frame Login Login
Replacement Consumables
64-0709 CS-24/50 Rectangular cover glass, #1 thickness, 24 x 50 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0273 SS-3 Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers Login Login
64-1533 SS-3G Mesh slice support for RC-27L, RC-29 recording chambers Login Login
Temperature Control
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
Accessories
64-1940 DWV Dedicated Workstation Vacuum Login Login
Replacement Parts
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-1533 SS-3G Mesh slice support for RC-27L, RC-29 recording chambers Login Login
64-0273 SS-3 Standard slice support (1 mm thread spacing) for RC-27L, RC-29 chambers Login Login

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