Specialized Tools for Electrophysiology and Cell Biology Research

NaviCyte Vertical Ussing System

The Navicyte system features the capability of running up to 6 chambers simultaneously and is optimized for transport studies on both filter grown monolayers and surgically excised tissue sections

  • Optimially designed for both electrophysiological and diffusion-only measurements
  • Employs reusable glass-barrel electrodes with ceramic frits
  • Supports up to 6 chambers in a single heated holder
  • Gas manifold with individual chamber delivery control
  • Supports membranes from 3 to 12 mm wide
  • Supports Snapwell culture cups
  • Compatible with UssingChart hardware and software

 

A complete NaviCyte vertical chamber system is comprised of up to six vertical chambers, a heated base assembly, a gas manifold, and (optionally) a data acquisition system. The standard base can hold up to six chambers and the included 12 channel gas manifold provides air regulation to both sides of each chamber. The included heater block allows for temperature control when used with a thermocirculator.

While well optimized for diffusion-based studies, the use of NaviCyte electrodes allows for electrophysiological membrane resistance measurements. Navicyte chambers (with the exception of the Snapwell) do not use inserts and the vertical line has 14 different chamber types available.

660008_Navicyte-Vertical_gr_1

Single or Multichannel Setups

Chambers can be used individually as a stand-alone entity, or can be mounted in quantity into the base assembly. The base assembly provides chamber heating and associated gas manifold allows up to six experiments to be run in parallel. The compact design (38.1 x 10.2 x 12.7 cm) of this setup conserves valuable bench space.

Heater Base

The standard heater block base provides efficient temperature control of the chambers. Temperature is regulated by use of an external, user supplied thermocirclator.

Gas Manifold

The 6-chamber (12-channel) gas manifold distributes gas to the chambers where media circulation is generated by a gas-lift process.

Standard Volume Chambers

NaviCyte Vertical chambers are primarily designed for work with excised tissue segments. Chambers are two piece assemblies held together by a high spring-tension retaining ring that insures leak free operation.

Tissues are affixed between the opposing faces of two half-chambers using a series of pins that surround the chamber aperture. Chambers are available with both circular and oblong openings of various sizes.

NaviCyte Vertical Ussing System

 

The optimal choice of opening will depend on the size and type of tissue under study. For example, an oblong opening can increase the effective sample surface area for an intestinal tissue.

The chamber aperture diameter designates the main opening to which buffer makes contact with the membrane. Generally speaking, you select an aperture opening at least 2 mm smaller (1 mm all the way around) than the tissue dimensions to permit the pins to impale the tissue without tearing.

 

SnapwellTM Chamber

The SnapwellTM chamber is specifically designed for use with SnapwellTM culture inserts from Corning Costar. This chamber accepts the lower section of the insert, which contains the cultured cell monolayer.

NaviCyte Vertical Ussing System

Low Volume Chambers

Several low volume chambers are offered and are designed to reduce the amount of compound required for permeability studies.

Perfusion Caps

Perfusion caps provide perfusion capability to the standard volume chambers. They are not needed for low volume chambers The use of a perfusion cap places a chamber into its 'closed' configuration.

Electrode Caps

Electrode caps provide electrode placement within the standard volume chambers. You do not need to use a perfusion cap if using an electrode cap. The low volume chambers do not use electrode caps and are supplied with electrode screws to position electrodes.

Note that there is a special electrode cap for the Snapwell chamber.

NaviCyte Electrodes

While optimized for diffusion-based assays, NaviCyte electrodes allow electrophysiology-based measurements with NaviCyte chambers. Standard volume chambers require the use of electrode caps for proper electrode placement. NaviCyte electrodes screw together for final assembly as shown in the image to the right.

NaviCyte Vertical Ussing System

Data Acquisition

The NaviCyte Vertical Ussing system is compatible with electrophysiological epithelial transport measurements and uses UssingChart data acquisition. This package includes an amplifier, a digitizer, software, and cables. You can view details on the UssingChart Data Acquisition webpage.

NaviCyte Vertical Ussing System

Navicyte Vertical Ussing System Expandability

The Navicyte vertical Ussing system can easily be scaled by adding chambers and expanding the UssingChart DAQ.
Refer to the chart below for needed components:

Expansion Needed items Order No. Quantity to add
2 to 4 channels - order all items
(does not include chambers - order 2 separately)
  EC-825A dual channel epithelial voltage clamp 64-1605 1
2 to 6 channels - order all items
(does not include chambers - order 4 separately)
  EC-825A dual channel epithelial voltage clamp 64-1605 2
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
2 to 8 channels - order all items
(does not include chambers - order 6 separately)
  6-Chamber heater block assembly 66-0075 1
  EC-825A dual channel epithelial voltage clamp 64-1605 3
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
4 to 6 channels - order all items
(does not include chambers - order 2 separately)
  EC-825A dual channel epithelial voltage clamp 64-1605 1
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
4 to 8 channels - order all items
(does not include chambers - order 4 separately)
  6-Chamber heater block assembly 66-0075 1
  EC-825A dual channel epithelial voltage clamp 64-1605 2
  LIH 8+8 digitizer 89-5035 1
  Dual EC-825A DAQ cable 64-3009 1
  LIH master/slave cable 89-5244 1
6 to 8 channels - order all items
(does not include chambers - order 2 separately)
  6-Chamber heater block assembly 66-0075 1
  EC-825A dual channel epithelial voltage clamp 64-1605 1
Material Acrylic

 

System Assembly

NaviCyte Vertical Ussing System

Chamber openings

NaviCyte Vertical Ussing System

For all chambers not using culture cups:

Increase the tissue dimensions by a minimum of 3.0 mm in each direction
to allow the o-ring or pins to capture the tissue without tearing.

For example, a chamber with a 5 mm round aperture will require a tissue with a minimum diameter of 8 mm,
and a chamber with a 2 x 10 mm oblong aperture will require a tissue with minimum dimension of 5 x 13 mm.

Suggested application(s) by aperture

Aperture Applications Aperture area
9 mm round with pins general use 0.34 cm2
4 mm round with pins general use 0.12 cm2
8 x24 mm oblong with pins rat or larger intestine
general use
1.78 cm2
4 x 8 mm oblong with pins rat or larger intestine
general use
0.29 cm2
6 x 9 mm oblong with pins rat and larger intestine
general use
0.46 cm2
5 x 24 mm oblong with pins rat and larger intestine
general use
1.15 cm2
5 mm round, low volume, with pins general use 0.20 cm2
3 mm round, low volume, with pins biopsy
general use
0.07 cm2
9 mm round, low volume, with pins general use 0.64 cm2
12 mm round, low volume, with pins general use 1.13 cm2
2 x 10 mm oblong, low volume, with pins mouse intestine
general use
0.10 cm2
4 x 8 mm oblong, low volume, with pins rat or larger intestine
general use
0.28 cm2

References

  1. Atorvastatin Transport in the Caco-2 Cell Model: Contributions of P-Glycoprotein and the Proton-Monocarboxylic Acid Co-Transporter 
    Wu X, Whitfield LR, Stewart BH
    Pharm Res. 2000 Feb;17(2):209-15
  2. In vitro measurement of gastrointestinal tissue permeability using a new diffusion cell 
    Grass, G.M. and Sweetana, S.A., 
    Pharm. Res. 5372-76 (1988)
  3. Characterization of the unstirred water layer in Caco-2 cell monolayers using a novel diffusion apparatus 
    Hidalgo, I.J., Hilgren, K.M., Grass, G.M., and Borchardt, R.T., 
    Pharm. Res. 8:222-227 (1991)

Pricing Top Description

How to order:

  • Select Base Assembly 
    Needed if using more than one chamber or if heating.
  • Select Chambers 
    Order from 1 to 6
  • Order Perfusion Caps 
    Not needed if making electrophysiological measurements or if using electrode caps. 
    Needed for perfusing in standard volume chambers. Not needed for low volume chambers. 
    One set per chamber.
  • Order Electrode Caps 
    Needed for making electrophysiological recordings in standard volume chambers. 
    Note special electrode cap for Snapwell chambers. 
    One set per chamber.
  • Order Electrodes and Glass Barrels 
    Needed for making electrophysiological recordings. 
    One electrode set per chamber.
  • Select Data Acquisition package for Ussing (UssingChart)
    If making electrophysiological measurements
  • Select Thermocirculator and Tubing Kit
    If needed - for temperature control

Order No. Model No. Product Description USA Price Order
6 Channel UssingChart package(order 3 of 64-1605; 2 each of 89-5035 and 64-3009)1 each of the rest
89-5244 LIH master/slave connecting cable Login Login
Accessories and Replacement Parts
66-0009 6-Chamber Heating Block Login Login
66-0048 12 Channel Air Manifold Login Login
66-0010 Clear front panel Login Login
66-0007 Navicyte retaining ring, 12 pack Login Login
66-0012 Retaining ring clamp tool Login Login
66-0050 Mounting screws for electrode caps, 12 pack Login Login
66-0057 Small o-rings for electrode screws, 24 pack Login Login
99-0403 Navicyte electrodes leads, 1 m long, set of 4 Login Login
750-7029 Replacement air manifold to chamber gas line, 6 inch long, each Login Login
Accessories and Replacement Parts - Thermocirulator
72-4924 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 120 V, 60 Hz Login Login
72-4921 TC-120 Thermocirculator, 5 Liter Plastic Bath with Lid, 220 V, 50 Hz Login Login
64-2244 WB-KIT TC-120 Thermocirculator Tubing Kit Login Login
Base Assembly
66-0075 6-Chamber heater block assembly (includes heating block, air/gas manifold, clear front panel, retaining ring clamp tool) Login Login
Chambers Standard Volume NaviCyte Vertical
66-0014 Standard Vertical Ussing/Diffusion Chamber, 9 mm diameter aperture, 0.64 cm2 area Login Login
66-0036 Standard Vertical Ussing/Diffusion Chamber, 4 mm diameter aperture, 0.12 cm2 area Login Login
66-0013 Standard Vertical Ussing/Diffusion Chamber, 8 x 24 mm aperture, 1.78 cm2 area Login Login
66-0032 Standard Vertical Ussing/Diffusion Chamber, 4 x 8 mm aperture, 0.29 cm2 area Login Login
66-0038 Standard Vertical Ussing/Diffusion Chamber, 6 x 9 mm aperture, 0.46 cm2 area Login Login
66-0046 Standard vertical Ussing/diffusion chamber, 5 x 24 mm aperture, 1.15 cm2 area Login Login
Data Acquisition
89-5035 LIH 8+8 LIH 8+8 computer interface, 90-250 VAC, 50/60 Hz, CE Login Login
Data Acquisition-2 Channel Data Acquisition package (UssingChart) - order 1 of each
64-1605 EC-825A Dual channel epithelial voltage clamp, ±50V compliance, 110-250 VAC Login Login
64-3009 EC-825A cable, dual - fits 2 amplifiers (4 acquisition channels) Login Login
89-5285 UssingChart software Login Login
Electrodes and Caps
66-0023 Ag/AgCl electrodes with KCl, 4 pack (sufficient for 1 chamber) Login Login
66-0024 Glass barrel for Ag/AgCl electrode, with ceramic tip, 8 pack Login Login
66-0019 Perfusion Cap for standard volume chamber , L/R pair Login Login
66-0018 Electrode Cap for standard volume chamber , L/R pair Login Login
66-0020 Electrode Cap for Snapwell chambers, L/R pair Login Login
66-0049 Open screws with o-rings for low volume chambers Serves as electrode holders, 12 pack Login Login
Low Volume NaviCyte Vertical
66-0026 Low volume vertical ussing/diffusion chamber, 5 mm diameter aperture, 0.20 cm2 area Login Login
66-0015 Low volume vertical ussing/diffusion chamber, 9 mm diameter aperture, 0.64 cm2 area Login Login
66-0027 Low volume vertical ussing/diffusion chamber, 12 mm diameter aperture, 1.13 cm2 area Login Login
66-0040 Low volume vertical ussing/diffusion chamber, 3 mm diameter aperture, 0.07 cm2 area Login Login
66-0034 Low volume vertical ussing/diffusion chamber, 2 x 10 mm aperture, 0.10 cm2 area Login Login
66-0042 Low volume vertical ussing/diffusion chamber, 4 x 8 mm aperture, 0.28 cm2 area Login Login
Specialty
66-0008 Specialty Vertical Ussing/Diffusion chamber, Snapwell, 1.13 cm2 Login Login

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