Specialized Tools for Electrophysiology and Cell Biology Research

(PMP-102) Programmable Pipette Puller

The PMP-102 is a state of the art microprocessor controlled horizontal pipette puller designed to simultaneously pull two identical, single barrel pipettes.

  • One touch, two identical pipettes every time
  • Optical-digital measurement
  • 25 saved programmable sequences
  • 22 manufacture preset sequences
  • Microprocessor real-time controlled system
  • Consistent pulling force by pneumatic pressure instead of gravity or magnetic field
  • Optional extra length PMP-102L can pull up to 120 mm capillaries

The PMP-102 is a state of the art, microprocessor-controlled pipette puller. The instrument is designed to pull pipettes horizontally and will produce two identical micropipettes. Various pipette styles can be repeatedly pulled via preset, programmed sequences. These include patch clamp and intracellular electrodes, microinjection micropipettes, and micro-needles. Many advanced technologies are built into the PMP-102 to achieve this versatility while preserving a high degree of reproducibility.

690151_PMP-102_clamp

Exclusive Optical-Digital Taper Measurement

Instead of using a mechanical tip length setting as is used in some other pipette pullers, The PMP-102 uses an exclusive optical-digital ruler to perform precise taper length measurements under real-time control. With this feature, a user can perform taper pulling very precisely and easily. Equipped with a powerful computerized tip sensing function, the puller can automatically pull a finished tip. This gives the user a quick reference to pull an ideal tip.

Computerized Real-Time Feedback Control

The PMP-102 features an advanced microcontroller to provide real-time heater monitoring and control. Once a heating level is selected and preset, the microcontroller measures the actual heating power during power on. The measurement is then displayed and fed back to the control unit to match the set point dynamically. As a result, the puller dynamically provides precise heating power regardless of the number of pull cycles or changes to the programming. If the heat level is set to AUTO, the heater will automatically search for the melting point of different pipette glasses.

Programmable, Savable Pulling Sequences

The PMP-102 can store up to 25 manufacturer and user programmed pulling sequences, with up to 18 steps in each sequence. Users can easily program for different pipette tip size, length, and shape, in a different sequence for each application. Time and length count, heat level, and action parameters can be individually set within each step. Once a sequence has been selected, two identical micropipettes will be automatically produced merely by pressing the START button.

Pneumatic Pulling Force

In spite of the instrument's compact size, the PMP-102 can precisely and automatically perform multistep pulls without a manual interrupt. Compared to pipette pullers using gravity or magnetic fields as the pulling force, the PMP-102 uses precisly controlled pneumatic pressure as the pulling force. This yeilds an even, more controled, and consistent dragging characteristic.

When performing a double horizontal pull, the PMP-102 can simultaneously pull two identical microinjection or microelectrode tips. Precision micro-linear ball bearing rail and advanced pneumatic components are used to provide fault-free pulling movements. A simple 4x4 keypad and full information display LCD lets users easily read and control all pulling parameters directly, which include sequences, steps, time, timing, heater level, heater control, tip length, and actions. The heater power control and acting time count-up or count-down are real-time displayed. With the versatility of the PMP-102, pulling an ideal micropipette is no longer an uncertainty of hand skill, but a reproducible, automatic process.

Manufacture's Preset Programs for Common Pipette Pulls

The PMP-102 is pre-installed with a number of common pipette pulling programs. The user can easily select the desired progran from sample pictures or from a characteristic list in the User's Manual. Input the corresponding sequence key and press START to initiate the automatic pull sequence. Preset programs are convenient and useful, not only because they can pull a variety of pipettes, but also because they can be used as templets to produce new programs. With the copy function and just a few modifications, a new customized sequence program can be configured to fit a special requirement. Up to 22 different sequences have been developed and are pre-set into the PMP-102 saving user's time and glass.

 
 

Optional Microforge Control

Since a microforge needs a precisely controlled heater and air jet, the PMP-102 has features to meet these requirements. The PMP-102 rear panel has an optional output connector which can supply computerized heater power allowing a user to easily set the heating level for a microforge heater as well as for the puller. The connector also supplies power for a microforge illumination light.

There are optional pressure output and foot switch connectors on the front panel which can be used to set the adjustable air pressure level for pipette tip polishing, shaping, or for heater cooling. Both microforge heater and air jet can be controlled by foot switches as well as by the key pad on the PMP-102 front panel. With the precise heater power supply, light power supply, and adjustable air pressure supply, a combination microforge can be built with less cost and more features.

Pipette Single barrel, 1.0-2.5 mm OD, borosilicate or aluminosilicate glass capillary tubing
Pulling force Pneumatic
Heater Nichrome coil
Heater control Microcontrollor
Heating 76 general heat levels (24-99), 54 automatic heat levels (45-98).
Number of sequences 25
Available steps per sequence 18
Taper length setting 0.5-20.0 mm
Pressure 1 regulator 0.1-10.0 psi
Pressure 2 regulator 0.1-60.0 psi
Cool air pressure 0.5-30.0 psi, adjustable
Pressure gas input 30-60 psi
Actions Pull 1, Pull 2, Pull 2/Cool, Cool Air, and Return.
Display 20x4 segment LCD
Heater power output Optional
Light power output Optional
Pressure air output Optional
Foot switch connector Optional, two (Heat Stop, Air Out)
Power output Heater supply, 12 VAC
Power input 110/240 VAC
Power consumption Maximum 150 watts
Dimension 18x8x11 inch, WxHxD
Weight 23 lbs.
Order No. Model No. Product Description USA Price Order
69-0154 IP-1 Input tubing with connector Login Login
69-0155 OP-1 Output tubing with connector and 4.5 ml vial Login Login
69-0156 OP-2 Output tubing with connector only Login Login
69-0157 FSW Footswitch Login Login
69-0158 PM-KP Remote key pad Login Login
69-0172 HC-2 Replacement heat coil for PMP-102 Login Login
Order No. Model No. Product Description USA Price Order
69-0151 PMP-102 PMP-102 (repl PMP-100D) programmable single barrel micropipette puller, 110-120VAC, 60 Hz Login Login
69-0151E PMP-102 PMP-102 (repl PMP-100D) programmable single barrel micropipette puller, 220-240VAC, 50/60 Hz Login Login
Accessories and Replacement Parts
69-0154 IP-1 Input tubing with connector Login Login
69-0155 OP-1 Output tubing with connector and 4.5 ml vial Login Login
69-0156 OP-2 Output tubing with connector only Login Login
69-0157 FSW Footswitch Login Login
69-0158 PM-KP Remote key pad Login Login
69-0172 HC-2 Replacement heat coil for PMP-102 Login Login

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