An effective method for applying silicone grease to recording chambers and 35 mm dish inserts. Sealing between coverslip and chamber is most often done with a high purity silicone lubricant such as stopcock or vacuum grease.
An artist's acrylic brush is an effective tool for applying silicone lubricant to a glass coverslip and polycarbonate chamber. This kit greatly simplifies the assembly of a Warner Recording and Perfusion Chambers.
This convenient package includes a tube of Dow Corning® 111 Valve Lubricant & Sealant, two acrylic paint brushes (sized #2 and #4), and several pallets. By "painting" the grease onto the bottom surface of a polycarbonate chamber, it is easy to evenly spread lubricant and create a water-tight seal.
Dow Corning® 111 Valve Lubricant & Sealant retains its viscosity at elevated temperatures better than others we tested and is particularly recommend for heated perfusion systems.