Specialized Tools for Electrophysiology and Cell Biology Research

(RC-1Z) Slotted Bath Oocyte Recording Chamber

A simple, low cost oocyte perfusion chamber

  • Specially designed for oocyte studies
  • Unique slotted bath
  • Dimpled bottom to secure oocyte into position

The RC-1Z chamber features a unique slot-shaped bath for applications requiring rapid perfusion.

The chamber is constructed using a two-piece design. The top piece forms the chamber body and contains a solution aspiration reservoir. An agar bridge well with connecting channel to the input side of the bath is also provided. An isolated well is provided on the downstream side of the bath, however, most users place the sense electrode directly into the suction well.

The bottom piece forms the floor of the chamber and houses the oocyte work area. To aid in securing the oocyte into position during impalement, the chamber bottom has three dimples with diameters of 0.8, 0.9, and 1.0 mm. Three grooves milled into the chamber bottom (parallel to the direction of solution flow) aid in bathing the bottom side of the oocyte during perfusion.

Solutions are removed from the suction well via an adjustable suction tube and bath solution height is adjusted by raising or lowering the suction tube. The perfusion input accepts standard 1/16" OD (PE-160) polyethylene tubing

Perfusion can be achieved by adding a VC-8 or VC-6 perfusion system, the DVW vacuum system takes care of the waste.

Material polycarbonate
Footprint
(L x W)
7.7 x 5.1 cm
Bath Dimensions
(L x W x H)
25 x 3.5 x 2.5 mm
Volume, by depth 85 μl/mm
Chamber Floor Insert 25 x 75 x 3 mm, polycarbonate
Input Tubing (ID x OD) 1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)
Order No. Model No. Product Description USA Price Order
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0206 MP-2 MP Series perfusion minimanifold, 2 to 1 ports Login Login
64-0207 MP-3 MP Series perfusion minimanifold, 3 to 1 ports Login Login
64-0208 MP-4 MP Series perfusion minimanifold, 4 to 1 ports Login Login
64-0209 MP-5 MP Series perfusion minimanifold, 5 to 1 ports Login Login
64-0210 MP-6 MP Series perfusion mini manifold, 6 to 1 ports Login Login
64-0211 MP-8 MP Series perfusion mini-manifold, 8 to 1 ports Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-1586 BPM-1 Base plate, magnetic steel, 12x24" x 3/8', thick powder coating Login Login

References

References

  1. A presynaptic endosomal trafficking pathway controls synaptic growth signaling.
    Rodal AA, Blunk AD, Akbergenova Y, Jorquera RA, Buhl LK, Littleton JT.
    J Cell Biol. 2011 Apr 4;193(1):201-17.
  2. DMob4/Phocein regulates synapse formation, axonal transport, and microtubule organization.
    Schulte J, Sepp KJ, Jorquera RA, Wu C, Song Y, Hong P, Littleton JT.
    J Neurosci. 2010 Apr 14;30(15):5189-203.
  3. Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains.
    Barber CF, Jorquera RA, Melom JE, Littleton JT.
    J Cell Biol. 2009 Oct 19;187(2):295-310. Epub 2009 Oct 12.
  4. Oviduct contraction in Drosophila is modulated by a neural network that is both, octopaminergic and glutamatergic.
    Rodríguez-Valentín R, López-González I, Jorquera R, Labarca P, Zurita M, Reynaud E.
    J Cell Physiol. 2006 Oct;209(1):183-98.
  5. Drosophila melanogaster Scramblases modulate synaptic transmission.
    Acharya U, Edwards MB, Jorquera RA, Silva H, Nagashima K, Labarca P, Acharya JK.
    J Cell Biol. 2006 Apr 10;173(1):69-82.
  6. A dopamine-gated ion channel (HcGGR3*) from Haemonchus contortus is expressed in the cervical papillae and is associated with macrocyclic lactone resistance
    Rao VT, Siddiqui SZ, Prichard RK, Forrester SG
    Mol Biochem Parasitol. 2009 Jul;166(1):54-61. Epub 2009 Mar 4
  7. Conformational changes in a pore-forming region underlie voltage-dependent "loop gating" of an unapposed connexin hemichannel
    Tang Q, Dowd TL, Verselis VK, Bargiello TA
    J Gen Physiol. 2009 Jun;133(6):555-70
  8. Loop Gating of Connexin Hemichannels Involves Movement of Pore-lining Residues in the First Extracellular Loop Domain
    Verselis VK, Trelles MP, Rubinos C, Bargiello TA, Srinivas M
    J Biol Chem. 2009 Feb 13;284(7):4484-93. Epub 2008 Dec 11
  9. Divalent Cations Regulate Connexin Hemichannels by Modulating Intrinsic Voltage-dependent Gating
    Verselis VK, Srinivas M
    J Gen Physiol. 2008 Sep;132(3):315-27. Epub 2008 Aug 11
  10. Morantel Allosterically Enhances Channel Gating of Neuronal Nicotinic Acetylcholine {alpha}3?2 Receptors
    Wu TY, Smith CM, Sine SM, Levandoski MM
    Mol Pharmacol. 2008 Aug;74(2):466-75. Epub 2008 May 5
  11. 1,4-Diazabicyclo[2.2.2]octane Derivatives: A Novel Class of Voltage-Gated Potassium Channel Blockers
    Gordon E, Cohen JL, Engel R, Abbott GW
    Mol Pharmacol. 2006 Mar;69(3):718-26. Epub 2005 Nov 29
  12. Regulation of Connexin Hemichannels by Monovalent Cations
    Srinivas M, Calderon DP, Kronengold J, Verselis VK
    J Gen Physiol. 2006 Jan;127(1):67-75
  13. Transport of the Natural Sweetener Stevioside and Its Aglycone Steviol by Human Organic Anion Transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8)
    Srimaroeng C, Chatsudthipong V, Aslamkhan AG, Pritchard JB
    J Pharmacol Exp Ther. 2005 May;313(2):621-8. Epub 2005 Jan 11
  14. Correlative Studies of Gating in Cx46 and Cx50 Hemichannels and Gap Junction Channels
    Srinivas M, Kronengold J, Bukauskas FF, Bargiello TA, Verselis VK
    Biophys J. 2005 Mar;88(3):1725-39. Epub 2004 Dec 13

Order No. Model No. Product Description USA Price Order
64-0107 TA-29 Cable with bead thermistor Login Login
64-0318 RC-1Z RC-1Z oocyte recording chamber Login Login
64-1402 ST-1Z Suction Tube, Classic Design for RC-1Z chamber Login Login
Accessories and Replacement Parts
64-0211 MP-8 MP Series perfusion mini-manifold, 8 to 1 ports Login Login
64-0208 MP-4 MP Series perfusion minimanifold, 4 to 1 ports Login Login
64-1586 BPM-1 Base plate, magnetic steel, 12x24" x 3/8', thick powder coating Login Login
64-0210 MP-6 MP Series perfusion mini manifold, 6 to 1 ports Login Login
64-0207 MP-3 MP Series perfusion minimanifold, 3 to 1 ports Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0209 MP-5 MP Series perfusion minimanifold, 5 to 1 ports Login Login
64-0206 MP-2 MP Series perfusion minimanifold, 2 to 1 ports Login Login
Plugs and Jacks
64-1327 WA10-5 Silver wire, 10 cm long, 0.25 mm dia, with 1 mm pin, 2 pack Login Login
Valve Control Systems
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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