Specialized Tools for Electrophysiology and Cell Biology Research

(RC-24N) Fast Exchange Open Diamond Bath

The RC-24N allows very fast solution exchange for a variety of applications. This recording chamber features the smallest bath volume of the open style chambers.

  • Designed for physiological measurements of cell cultured coverslips
  • Applications include patch clamp, intracellular/extracellular recordings, and imaging
  • Diamond shaped bath provides laminar solution flow
  • Very small bath volume facilitates very fast solution exchange

The RC-24N chamber design is taken from the RC-24 and improves the flow characteristics by providing a linear path. The bath volume in this model is among the smallest of the open style chambers. This enables very fast solution exchange for a variety of applications, including patch clamp and electrophysiology studies. The small bath volume is especially useful when using drugs in small quantities.

An agar bridge well for reference electrodes is provided, as is an aspiration well. The aspiration well is equipped with a stainless steel aspiration tube (1.67 mm OD) together with a tube clamp. The clamp insures that the adjustable suction tube will not move once the height is set by the user. The floor of this chamber is formed from a 22 mm x 40 mm rectangular coverslip.

The RC-24N requires a platform (PM-1PH-1 or P-1) and Series 20 stage adapter to complete the assembly for mounting on a microscope.

Each chamber is supplied with a package of #1 thickness glass coverslips (CS-22/40, 22 x 40 mm, 50/pkg) and 10 ft of PE-160 tubing.

Material Polycarbonate
Bath dimensions
(L x W x H)
15 x 6 x 3.8 mm
Volume, by depth 50 μl/mm
Bottom coverglass 22 x 40 mm
Input tubing
(ID x OD)
1.14 x 1.57 mm (PE-160)
Aspirator coupling 1.67 mm (OD)

 

 

Order No. Model No. Product Description USA Price Order
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

Videos

  1. Perfusion and Aspiration Techniques
    Warner Instruments
    YouTube 2012

References

  1. Orexin-A-induced Ca2+ Entry EVIDENCE FOR INVOLVEMENT OF TRPC CHANNELS AND PROTEIN KINASE C REGULATION*,
    Kim P. Larsson, Hanna M. Peltonen, Genevieve Bart, Lauri M. Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P. Kukkonen, and Karl E. O. Åkerman,
    J. Biol. Chem., Vol. 280, Issue 3, 1771-1781, January 21, 2005
  2. Stimulation of astrocyte Na+/H+ exchange activity in response to in vitro ischemia depends in part on activation of ERK1/2,
    Douglas B. Kintner, Andy Look, Gary E. Shull, and Dandan Sun,
    Am J Physiol Cell Physiol 289: C934-C945, 2005
  3. Decreased Neuronal Death in Na+/H+ Exchanger Isoform 1-Null Mice after In Vitro and In Vivo Ischemia,
    Jing Luo, Hai Chen, Douglas B. Kintner, Gary E. Shull, and Dandan Sun,
    The Journal of Neuroscience, December 7, 2005, 25(49):11256-11268
Order No. Model No. Product Description USA Price Order
64-0381 RC-24N Fast exchange recording chamber, narrow bath, 50 µl volume Login Login
Platforms
64-1526 PM-1 PM-1 Platform for Series 20 chambers, heated, magnetic clamps Login Login
64-0277 P-1 P-1 Platform for Series 20 chambers, non-heater Login Login
Accessories and Replacement Parts
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1506 RPC-1 Replacement platform clamps and screws for P and PH Series platforms, 2 pack Login Login
64-1920 SA-20PS1 Adapter Ring for Pecon Heating Insert P Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Standard Dimension Coverslips: #1 Thickness (0.15 ± 0.02 mm)
64-0707 CS-22/40 Rectangular cover glass, #1 thickness, 22 x 40 mm, 50 pack Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

Need support?

Want to know more about our quality products or need assistance in technical support?

TALK TO OUR EXPERTS

NEWSLETTER

For the most up-to-date information on Warner Instruments news and technologies, sign-up for our newsletter.