Specialized Tools for Electrophysiology and Cell Biology Research

(RC-27D, RC-27LD) Ultra-quiet Imaging Chambers for Slice Studies

The RC-27D and RC-27LD are designed for applications requiring ultra quiet solution delivery.

  • Designed for tissue slice studies
  • RC-27LD includes a slice support
  • Sloped input eliminates vibration associated with solution delivery
  • Alows multiplexed solution exchange without switching artifacts

The large imaging field in the RC-27D and RC-27LD allows for maximal observation of biological specimens with the addition of a sloped input. The advantages of the sloped inflow channel includes smooth and "ultra-quiet" solution delivery.

The sloped input allows I-V type infusion lines to deliver drops at a fixed rate without the transfer of noise to the specimen field. In addition, multiplexed solution delivery is now possible without the potential for solution switching artifacts. This technology provides the capability of ultra-quiet solution exchange without transferring noise to either the system or to the image.

Ultra-quiet solution delivery when measuring from tissue slices is provided by the inclusion of a slice support in the RC-27LD chamber. Slices are supported 0.5 mm above the chamber floor. Support threads are made of Lycra and thread spacing 0.5 mm.

 

The RC-27D requires a PM-7 platform while the RC-27LD requires a PM-7Dplatform.

Both platforms are compatible our Series 20 stage adapters which complete the assembly for mounting onto a microscope.

Both chambers are supplied with a MAG-2 magnetic clamp for suction tubes, a magnetic perfusion tube holder, a tissue slice support, a package of #2 thickness glass coverslips (CS-24/60), 40/pkg, and 10 feet of PE-160 polyethylene tubing. The RC-27LD also includes a slice support. Slice anchors are sold separately.

 

Material Polycarbonate
Bath dimensions
(L x W x H)
38 x 18 x 2.5 mm
Outer dimensions
(L x W x H)
59.9 x 25.4 x 4.2 mm
Volume, by depth 560 µl/mm
Bottom coverglass 24 x 60 mm


Order No. Model No. Product Description USA Price Order
64-0710 CS-24/60 Rectangular cover glass, #2 thickness, 24 x 60 mm, 50 pack Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1550 SS-4-500V Slice support, 500 um vertical thread spacing, for RC-27D, RC-27LD drip chambers Login Login
64-1551 SS-4-500H Slice support, 500 um horizontal thread spacing, for RC-27D, RC-27LD drip chambers Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login

References

References

  1. Cholecystokinin Activates Orexin/Hypocretin Neurons through the Cholecystokinin A Receptor
    Tsujino N, Yamanaka A, Ichiki K, Muraki Y, Kilduff TS, Yagami K, Takahashi S, Goto K, Sakurai T
    J Neurosci. 2005 Aug 10;25(32):7459-69
  2. Chronic Cocaine Administration Switches Corticotropin-Releasing Factor2 Receptor-Mediated Depression to Facilitation of Glutamatergic Transmission in the Lateral Septum
    Liu J, Yu B, Orozco-Cabal L, Grigoriadis DE, Rivier J, Vale WW, Shinnick-Gallagher P, Gallagher JP
    J Neurosci. 2005 Jan 19;25(3):577-83
Order No. Model No. Product Description USA Price Order
Chambers
64-1548 RC-27D Ultra Quiet Imaging Chamber, large bath, IV drip type Login Login
64-1548 RC-27D Ultra Quiet Imaging Chamber, large bath, IV drip type Login Login
64-1532 RC-27LD Ultra Quiet Imaging Chamber, very large bath, IV drip type, with slice support Login Login
64-1529 PM-7 PM-7 Platform for Series 20 chambers, magnetic clamps, heated Login Login
64-1530 PM-7D PM-7D Platform for Series 20 chambers, magnetic clamps, heated Login Login
Accessories and Replacement Parts
64-1550 SS-4-500V Slice support, 500 um vertical thread spacing, for RC-27D, RC-27LD drip chambers Login Login
64-0755 PE-160/10 Polyethylene tubing, PE-160, 10 ft Login Login
64-0756 PE-160/100 Polyethylene tubing, PE-160, 100 ft Login Login
64-1450 CPS-27L Perfusion spreader plate for RC-27L Login Login
64-1550 SS-4-500V Slice support, 500 um vertical thread spacing, for RC-27D, RC-27LD drip chambers Login Login
64-1551 SS-4-500H Slice support, 500 um horizontal thread spacing, for RC-27D, RC-27LD drip chambers Login Login
64-3018 PM-plate Pressure plates for PM Series platforms, 2 pack Login Login
64-0106 CC-28 Cable assembly for heater controllers to platform Login Login
64-0107 TA-29 Cable with bead thermistor Login Login
64-0274 RH-2 Heater elements for Warner chambers and platforms, 2 pack Login Login
Series 20 Slice Anchor Kits
64-0268 SHD-27LKIT Slice anchor kit for RC-27L, RC-27D, RC-29, stainless steel Login Login
64-0190 SHD-27LPKIT Slice anchor kit for RC-27L, RC-27D, RC-29, plastic frame Login Login
RC-27L / RC-27D / RC-29 Chamber Slice Anchors
64-0256 SHD-27LH/10 Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, stainless steel Login Login
64-0257 SHD-27LH/15 Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, stainless steel Login Login
64-0259 SHD-27LP/10 Slice anchor for RC-27L, RC-27D, RC-29, 1.0 mm thread spacing, plastic frame Login Login
64-0260 SHD-27LP/15 Slice anchor for RC-27L, RC-27D, RC-29, 1.5 mm thread spacing, plastic frame Login Login
64-0261 SHD-27LP/2 Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, plastic frame Login Login
Slice Anchors
64-0258 SHD-27LH/2 Slice anchor for RC-27L, RC-27D, RC-29, 2.0 mm thread spacing, stainless steel Login Login
Standard Dimension Coverslips: #2 Thickness (0.22 ± 0.02 mm)
64-0710 CS-24/60 Rectangular cover glass, #2 thickness, 24 x 60 mm, 50 pack Login Login
Heater Controllers
64-2401 TC-344C Heater controller, dual channel, 110-230 VAC, 50/60Hz Login Login
64-2400 TC-324C Heater controller, single channel, 110-230 VAC, 50/60Hz Login Login
Valve Control Systems
64-0185 VC-8P Perfusion valve control system, complete, with pinch valves, 8 channel Login Login
64-0135 VC-66CS Perfusion valve control system, complete, with pinch valves, 6 channel Login Login
64-1940 DWV Dedicated Workstation Vacuum Login Login

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